Aquifex aeolicus VF5 Pathway: UDP-N-acetyl-D-glucosamine biosynthesis I

Pathway diagram: UDP-N-acetyl-D-glucosamine biosynthesis I

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Synonyms: UDP-N-acetyl-D-glucosamine biosynthesis

Superclasses: Biosynthesis Amines and Polyamines Biosynthesis UDP-N-acetyl-D-glucosamine Biosynthesis
Biosynthesis Cell Structures Biosynthesis Lipopolysaccharide Biosynthesis O-Antigen Biosynthesis

Pathway Summary from MetaCyc:
UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) is an essential precursor of cell wall peptidoglycan, lipopolysaccharide and enterobacterial common antigen. This situates UDP-GlcNAc at a branch point in metabolism, each fork leading to synthesis of a major envelope component of the cell [Neidhardt96]. The enzymes of these pathways are targets for development of novel antibacterial compounds (reviewed in [Kotnik07]).

L-glutamine:D-fructose-6-phosphate aminotransferase, or GFAT, catalyzes the first committed step in UDP-GlcNAc biosynthesis from fructose-6-phosphate. The product of this first reaction, D-glucosamine 6-phosphate, can also be transported into the cell and utilized as a source of carbon; thus, expression of GFAT is controlled at several levels. For details on regulation and the mechanism of uncoupling it from the regulation of glmU expression, please see the protein page: L-glutamine:D-fructose-6-phosphate aminotransferase.

D-glucosamine 6-phosphate is then converted to D-glucosamine 1-phosphate by phosphoglucosamine mutase. The two final reactions of the pathway, transfer of an acetyl group from acetyl-CoA to form N-acetyl-α-D-glucosamine 1-phosphate and transfer of a uridyl group to form the final product, UDP-GlcNAc, are carried out by a bifunctional enzyme, GlmU, which contains two domains that carry out each reaction independently.

Pathway Evidence Glyph:

Pathway evidence glyph

Key to pathway glyph edge colors: ?

  An enzyme catalyzing this reaction is present in this organism
  An enzyme catalyzing this reaction was identified in this organism by the Pathway Hole Filler
  No enzyme catalyzing this reaction has been identified in this organism
  The reaction and any enzyme that catalyzes it (if one has been identified) is unique to this pathway

Imported from MetaCyc 08-Aug-2014 by Subhraveti P , SRI International


Kotnik07: Kotnik M, Anderluh PS, Prezelj A (2007). "Development of novel inhibitors targeting intracellular steps of peptidoglycan biosynthesis." Curr Pharm Des 13(22);2283-309. PMID: 17692001

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Green04: Green ML, Karp PD (2004). "A Bayesian method for identifying missing enzymes in predicted metabolic pathway databases." BMC Bioinformatics 5;76. PMID: 15189570

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Rubio06: Rubio S, Larson TR, Gonzalez-Guzman M, Alejandro S, Graham IA, Serrano R, Rodriguez PL (2006). "An Arabidopsis mutant impaired in coenzyme A biosynthesis is sugar dependent for seedling establishment." Plant Physiol 140(3);830-43. PMID: 16415216

Stryer88: Stryer L "Biochemistry." WH Freeman and Co., 3rd edition, New York, 1988.

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