|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
In Pathway: reductive TCA cycle II
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 2-oxoglutarate carboxylase
Enzyme Commission Synonyms: oxalosuccinate synθse, carboxylating factor for ICDH (incorrect), CFI, OGC
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -9.341309 [Latendresse13]
Enzyme Commission Summary:
A biotin-containing enzyme that requires Mg2+ for activity. It was originally thought [Aoshima04] that this enzyme was a promoting factor for the carboxylation of 2-oxoglutarate by EC 22.214.171.124, isocitrate dehydrogenase (NAD+), but this has since been disproved [Aoshima06]. The product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 126.96.36.199 [Aoshima06].
Aoshima04: Aoshima M, Ishii M, Igarashi Y (2004). "A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6." Mol Microbiol 51(3);791-8. PMID: 14731279
Aoshima06: Aoshima M, Igarashi Y (2006). "A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6." Mol Microbiol 62(3);748-59. PMID: 17076668
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