|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
In Pathway: guanine and guanosine salvage II
Note that this reaction equation differs from the official Enzyme Commission reaction equations for this EC number.
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: Balanced.
Enzyme Commission Primary Name: purine nucleosidase
Enzyme Commission Synonyms: nucleosidase (misleading), purine β-ribosidase, purine nucleoside hydrolase, purine ribonucleosidase, ribonucleoside hydrolase (misleading), nucleoside hydrolase (misleading), N-ribosyl purine ribohydrolase, nucleosidase g, N-D-ribosylpurine ribohydrolase, inosine-adenosine-guanosine preferring nucleoside hydrolase, purine-specific nucleoside N-ribohydrolase, IAG-nucleoside hydrolase, IAG-NH
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -0.73301315 [Latendresse13]
Enzyme Commission Summary:
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [Ogawa01].
MazumderShivaku05: Mazumder-Shivakumar D, Bruice TC (2005). "Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues." Biochemistry 44(21);7805-17. PMID: 15909995
Ogawa01: Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S (2001). "Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi." Appl Environ Microbiol 67(4);1783-7. PMID: 11282633
Versees02: Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J (2002). "Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax." J Biol Chem 277(18);15938-46. PMID: 11854281
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