|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 22.214.171.124
In Pathway: guanine and guanosine salvage II
Note that this reaction equation differs from the official Enzyme Commission reaction equations for this EC number.
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Mass balance status: Balanced.
Enzyme Commission Primary Name: purine nucleosidase
Enzyme Commission Synonyms: nucleosidase (misleading), purine β-ribosidase, purine nucleoside hydrolase, purine ribonucleosidase, ribonucleoside hydrolase (misleading), nucleoside hydrolase (misleading), N-ribosyl purine ribohydrolase, nucleosidase g, N-D-ribosylpurine ribohydrolase, inosine-adenosine-guanosine preferring nucleoside hydrolase, purine-specific nucleoside N-ribohydrolase, IAG-nucleoside hydrolase, IAG-NH
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -0.73301315 [Latendresse13]
Enzyme Commission Summary:
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [Ogawa01].
MazumderShivaku05: Mazumder-Shivakumar D, Bruice TC (2005). "Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues." Biochemistry 44(21);7805-17. PMID: 15909995
Ogawa01: Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S (2001). "Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi." Appl Environ Microbiol 67(4);1783-7. PMID: 11282633
Versees02: Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J (2002). "Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax." J Biol Chem 277(18);15938-46. PMID: 11854281
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