If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Locations of Mapped Genes:
Synonyms: ammonia assimilation III, GS/GOGAT pathway
|Superclasses:||Degradation/Utilization/Assimilation → Inorganic Nutrients Metabolism → Nitrogen Compounds Metabolism → Ammonia Assimilation|
Escherichia coli K-12 can synthesize L-glutamate from ammonia in two pathways. In addition, if complex sources of nitrogen are available, other pathways become active and take over glutamate synthesis.
The other pathway, which is described here, is a cyclic pathway, which consists of two steps.
Because glutamine biosynthesis utilizes one molecule of glutamate and the second reaction yields 2 molecules of glutamate, these two reactions function as a cycle, each turn of which utilizes one molecule each of ammonia, ATP, NADPH, and 2-oxoglutarate, and produces one molecule of L-glutamate.
This pathway is active in energy-rich (glucose-containing), nitrogen-poor environments.
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