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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Caulobacter crescentus CB15 Pathway: L-glutamine biosynthesis II (tRNA-dependent)

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Synonyms: glutaminyl-tRNAgln biosynthesis via transamidation

Superclasses: Biosynthesis Amino Acids Biosynthesis Individual Amino Acids Biosynthesis Glutamine Biosynthesis
Biosynthesis Aminoacyl-tRNA Charging

Pathway Summary from MetaCyc:
The production of L-glutaminyl-tRNAgln can occur in two different ways. In Gram-negative bacteria and in the cytosol of eukaryotic cells, there are separate tRNA synthetases for charging tRNAgln with L-glutamine and tRNAGlu with L-glutamate (see tRNA charging). However, Gram-positive bacteria (such as Bacillus subtilis), archaea and the chloroplasts and mitochondria of eukaryotic cells lack a synthetase for charging tRNAgln with L-glutamine (although in some protists and fungi, it is imported from the cytosol into mitochondria). This results in a misacylation of tRNAgln with L-glutamate by a nondiscriminating glutamyl-tRNAGlx synthetase. A second enzyme, glutamyl-tRNAGln amidotransferase, is used to transamidate the misacylated L-glutamyl-tRNAGln to L-glutaminyl-tRNAgln. In [Curnow97] and in [Pujol08]).

Other tRNA-dependent amino acid transformations have been reported for asparaginyl-tRNA, selenocysteinyl-tRNA and formylmethionyl-tRNA (reviewed in [Ibba97]).

Variants: glutamine biosynthesis I

Pathway Evidence Glyph:

Key to pathway glyph edge colors: ?

  An enzyme catalyzing this reaction is present in this organism
  No enzyme catalyzing this reaction has been identified in this organism
  The reaction and any enzyme that catalyzes it (if one has been identified) is unique to this pathway

Credits:
Created 07-May-2008 by Fulcher CA , SRI International


References

Curnow97: Curnow AW, Hong K, Yuan R, Kim S, Martins O, Winkler W, Henkin TM, Soll D (1997). "Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation." Proc Natl Acad Sci U S A 94(22);11819-26. PMID: 9342321

Ibba97: Ibba M, Curnow AW, Soll D (1997). "Aminoacyl-tRNA synthesis: divergent routes to a common goal." Trends Biochem Sci 22(2);39-42. PMID: 9048478

Pujol08: Pujol C, Bailly M, Kern D, Marechal-Drouard L, Becker H, Duchene AM (2008). "Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants." Proc Natl Acad Sci U S A 105(17);6481-5. PMID: 18441100


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Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.