Caulobacter crescentus CB15 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
thiamine-phosphate pyrophosphorylase: thiE

In Pathway: thiamin salvage II

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: thiamine phosphate synthase

Enzyme Commission Synonyms: thiamine phosphate pyrophosphorylase, thiamine monophosphate pyrophosphorylase, TMP-PPase, thiamine-phosphate diphosphorylase

Enzyme Commission Summary:
The enzyme catalyses the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components. The enzyme is thought to accept the product of EC, thiazole synthase, as its substrate. However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, thiazole tautomerase ( EC converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase. In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphono-oxyethyl)thiazole, which is produced from thiamine degradation products. In yeast this activity is found in a bifunctional enzyme (TH6) and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme (TH1).

Citations: [Leder61, Camiener60, Kawasaki93, Chiu99, Ajjawi07, Backstrom95]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Ajjawi07: Ajjawi I, Tsegaye Y, Shintani D (2007). "Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1." Arch Biochem Biophys 459(1);107-14. PMID: 17174261

Backstrom95: Backstrom AD, McMordie RAS, Begley TP (1995). "Biosynthesis of Thiamin I: The Function of the thiE Gene Product." J Am Chem Soc 117: 2351-2352.

Camiener60: Camiener GW, BrownN GM (1960). "The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates." J Biol Chem 235;2411-7. PMID: 13807175

Chiu99: Chiu HJ, Reddick JJ, Begley TP, Ealick SE (1999). "Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution." Biochemistry 38(20);6460-70. PMID: 10350464

Kawasaki93: Kawasaki Y (1993). "Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway." J Bacteriol 175(16);5153-8. PMID: 8394314

Leder61: Leder IG (1961). "The enzymatic synthesis of thiamine monophosphate." J Biol Chem 236;3066-71. PMID: 14463407

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