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Caulobacter crescentus CB15 Reaction: 5.4.99.12


Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-ReactionstRNA-Reactions

EC Number: 5.4.99.12

Enzymes and Genes:
tRNA pseudouridine synthase: truA
tRNA pseudouridine synthase B: truB

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA pseudouridine38-40 synthase

Enzyme Commission Synonyms: TruA, tRNA pseudouridine synthase I, PSUI, hisT (gene name)

Enzyme Commission Summary:
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. The bacterial enzyme TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures [Hur07]. The yeast enzyme Pus3 is active only towards uridine38 and uridine39, and shows no activity with uridine40 [Zhao97]. The enzyme from mouse is active only towards uridine39 [Foster00].

Citations: [Huang98a, Kammen88, Turnbough79, Dong06, Arena78, Lecointe98]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:5.4.99.12, ENZYME:EC:5.4.99.12, IUBMB-ExplorEnz:EC:5.4.99.12


References

Arena78: Arena F, Ciliberto G, Ciampi S, Cortese R (1978). "Purification of pseudouridylate synthetase I from Salmonella typhimurium." Nucleic Acids Res 5(12);4523-36. PMID: 370771

Dong06: Dong X, Bessho Y, Shibata R, Nishimoto M, Shirouzu M, Kuramitsu S, Yokoyama S (2006). "Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8." RNA Biol 3(3);115-22. PMID: 17114947

Foster00: Foster PG, Huang L, Santi DV, Stroud RM (2000). "The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I." Nat Struct Biol 7(1);23-7. PMID: 10625422

Huang98a: Huang L, Pookanjanatavip M, Gu X, Santi DV (1998). "A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst." Biochemistry 37(1);344-51. PMID: 9425056

Hur07: Hur S, Stroud RM (2007). "How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA." Mol Cell 26(2);189-203. PMID: 17466622

Kammen88: Kammen HO, Marvel CC, Hardy L, Penhoet EE (1988). "Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I." J Biol Chem 263(5);2255-63. PMID: 3276686

Lecointe98: Lecointe F, Simos G, Sauer A, Hurt EC, Motorin Y, Grosjean H (1998). "Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop." J Biol Chem 273(3);1316-23. PMID: 9430663

Turnbough79: Turnbough CL, Neill RJ, Landsberg R, Ames BN (1979). "Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium." J Biol Chem 254(12);5111-9. PMID: 376505

Zhao97: Zhao X, Horne DA (1997). "The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I." J Biol Chem 272(3);1950-5. PMID: 8999885


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