Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Caulobacter crescentus CB15 Reaction: 5.4.99.12

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number: 5.4.99.12

Enzymes and Genes:
tRNA pseudouridine synthase : truA
tRNA pseudouridine synthase B : truB

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA pseudouridine38-40 synthase

Enzyme Commission Synonyms: TruA, tRNA pseudouridine synthase I, PSUI, hisT (gene name)

Enzyme Commission Summary:
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. The bacterial enzyme TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures [Hur07]. The yeast enzyme Pus3 is active only towards uridine38 and uridine39, and shows no activity with uridine40 [Zhao97]. The enzyme from mouse is active only towards uridine39 [Foster00].

Citations: [Huang98a, Kammen88, Turnbough79, Dong06, Arena78, Lecointe98]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:5.4.99.12 , ENZYME:EC:5.4.99.12 , IUBMB-ExplorEnz:EC:5.4.99.12


References

Arena78: Arena F, Ciliberto G, Ciampi S, Cortese R (1978). "Purification of pseudouridylate synthetase I from Salmonella typhimurium." Nucleic Acids Res 5(12);4523-36. PMID: 370771

Dong06: Dong X, Bessho Y, Shibata R, Nishimoto M, Shirouzu M, Kuramitsu S, Yokoyama S (2006). "Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8." RNA Biol 3(3);115-22. PMID: 17114947

Foster00: Foster PG, Huang L, Santi DV, Stroud RM (2000). "The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I." Nat Struct Biol 7(1);23-7. PMID: 10625422

Huang98a: Huang L, Pookanjanatavip M, Gu X, Santi DV (1998). "A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst." Biochemistry 37(1);344-51. PMID: 9425056

Hur07: Hur S, Stroud RM (2007). "How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA." Mol Cell 26(2);189-203. PMID: 17466622

Kammen88: Kammen HO, Marvel CC, Hardy L, Penhoet EE (1988). "Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I." J Biol Chem 263(5);2255-63. PMID: 3276686

Lecointe98: Lecointe F, Simos G, Sauer A, Hurt EC, Motorin Y, Grosjean H (1998). "Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop." J Biol Chem 273(3);1316-23. PMID: 9430663

Turnbough79: Turnbough CL, Neill RJ, Landsberg R, Ames BN (1979). "Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium." J Biol Chem 254(12);5111-9. PMID: 376505

Zhao97: Zhao X, Horne DA (1997). "The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I." J Biol Chem 272(3);1950-5. PMID: 8999885


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