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Escherichia coli K-12 substr. MG1655 tRNA Class: a tRNAarg

Synonyms: TRNA(ARG)

Superclasses: an uncharged tRNA

Instances:
tRNAargQ ,
tRNAargU ,
tRNAargV ,
tRNAargW ,
tRNAargX ,
tRNAargY ,
tRNAargZ

Summary:
tRNAarg is the tRNA molecule that binds L-arginine.

A transfer RNA (tRNA) is an adaptor molecule made out of RNA that serves as the physical link between the nucleotide sequence of nucleic acids (DNA and RNA) and the amino acid sequence of proteins.

There are many tRNAs, most of which are specific for a particular amino acid. Each tRNA binds its amino acid and contains a three-nucleotide sequence called the anticodon, which forms three base pairs with a matching codon in mRNA during protein biosynthesis.

The amino acid is covalently attached to the terminal phosphate group of a CCA motif present at the 3'-end of the tRNA. More specifically, the phosphate is attached to position 5 of the ribose of the terminal adenine ribonucleotide of the tRNA. This covalent attachment is catalyzed by dedicated aminoacyl-tRNA synthetases [Ibba00]. The bound amino acid is then delivered to the ribosome by proteins called elongation factors (EF-Tu in bacteria, eEF-1 in eukaryotes).

The structure shown here depicts the phosphate group at the 3' end of the tRNA molecule, which binds the amino acid (in the form of an amino-acyl group).

a tRNA<SUP>arg</SUP> compound structure

Reactions known to consume the compound:

tRNA charging :
a tRNAarg + L-arginine + ATP + H+ → an L-arginyl-[tRNAarg] + AMP + diphosphate

Reactions known to produce the compound:

tRNA processing :
a tRNA precursor with a short 3' extension → an uncharged tRNA + n a nucleoside 5'-monophosphate
a tRNA precursor with a short 3' extension + n phosphate → an uncharged tRNA + n a ribonucleoside diphosphate
a tRNA precursor with a 5' extension + H2O → an uncharged tRNA + a single-stranded RNA

Not in pathways:
an N-modified aminoacyl-[tRNA] + H2O → an uncharged tRNA + an N-modified amino acid + 2 H+
a D-aminoacyl-[tRNA] + H2O → a D-amino acid + an uncharged tRNA + 2 H+

Not in pathways:
a tRNA precursor + H2O → a tRNA + a nucleoside 5'-monophosphate

tRNA processing :
a tRNA precursor with a 5' extension and a short 3' extension + H2O → a tRNA precursor with a short 3' extension + a single-stranded RNA
a tRNA precursor with a 5' extension + H2O → an uncharged tRNA + a single-stranded RNA

Not in pathways:
YhaV endonuclease degradation substrate mRNA + H2O → 2 a single-stranded RNA
an mRNA + H2O → a single-stranded RNA + a single-stranded RNA
an mRNA + H2O → a single-stranded RNA + a single-stranded RNA
RNase E degradation substrate mRNA + n H2O → n a single-stranded RNA
YhaV endonuclease degradation substrate rRNA + H2O → 2 a single-stranded RNA
RNase III mRNA processing substrate + 2 H2O → RNase III processing product mRNA + 2 a single-stranded RNA
23S rRNA[periplasmic space] + H2O[periplasmic space] → 2 a single-stranded RNA[periplasmic space]
an mRNA[periplasmic space] + H2O[periplasmic space] → 2 a single-stranded RNA[periplasmic space]
RNase G degradation substrate mRNA + H2O → 2 a single-stranded RNA
9S rRNA + 2 H2O → 5S rRNA + 2 a single-stranded RNA
RNase E mRNA processing substrate + n H2O → RNase E processing product mRNA + n a single-stranded RNA

Reactions known to both consume and produce the compound:

Not in pathways:
a single-stranded RNA + phosphate ↔ a single-stranded RNA + a nucleoside diphosphate

In Reactions of unknown directionality:

Not in pathways:
rRNA[periplasmic space] = 2 a single-stranded RNA[periplasmic space]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: IAF1260:38054 , KEGG:C01636


References

Ibba00: Ibba M, Soll D (2000). "Aminoacyl-tRNA synthesis." Annu Rev Biochem 69;617-50. PMID: 10966471


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Jul 30, 2015, biocyc14.