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Escherichia coli K-12 substr. MG1655 Compound: Cu2+

Synonyms: Cu+2, Cu++, cupric ion, cupric copper, Cu(II)

Superclasses: an ion a cation

Component of:
copper sulfate pentahydrate
copper sulfate

Chemical Formula: Cu

Molecular Weight: 63.546 Daltons

Monoisotopic Molecular Weight: 64.9452511642 Daltons

SMILES: [Cu++]

InChI: InChI=1S/Cu/q+2

InChIKey: InChIKey=JPVYNHNXODAKFH-UHFFFAOYSA-N

Unification Links: ChEBI:29036 , ChemSpider:25221 , HMDB:HMDB00657 , IAF1260:50600 , PubChem:27099

Standard Gibbs Free Energy of Change Formation (ΔfG in kcal/mol): 0.0

Reactions known to produce the compound:

Not in pathways:
4 Cu+[periplasmic space] + 4 H+[periplasmic space] + oxygen[periplasmic space] → 4 Cu2+[periplasmic space] + 2 H2O[periplasmic space]

In Reactions of unknown directionality:

Not in pathways:
Cu2+ + NADH = Cu+ + NAD+ + H+

In Transport reactions:
Cu2+[periplasmic space]Cu2+[cytosol]

Enzymes inhibited by Cu2+, sorted by the type of inhibition, are:

Inhibitor (Competitive) of: cytosine deaminase [Porter93, Comment 1]

Inhibitor (Mechanism unknown) of: N-acetyl-β-neuraminate lyase [Aisaka91, Uchida84] , glyoxalase [Subedi11] , NADPH-dependent curcumin reductase [Hassaninasab11] , guanosine kinase [Mori95] , inosine kinase [Mori95] , glutaminase [Hartman68] , 7-α-hydroxysteroid dehydrogenase [Yoshimoto91] , L-serine deaminase [Newman80a] , S-formylglutathione hydrolase [Gonzalez06] , S-formylglutathione hydrolase [Gonzalez06] , mannose isomerase [Itoh08] , deoxyribose 1,5-phosphomutase [HammerJespersen70] , 3'-nucleotidase [Anraku64] , glutamate decarboxylase B [Helmward89] , crotonobetainyl-CoA reductase [Roth94] , 3-deoxy-D-manno-octulosonate 8-phosphate synthase [Ray80] , glycogen phosphorylase [Chen68a, Chen68] , N-acetylglutamylphosphate reductase [Vogel74] , trimethylamine N-oxide reductase [Shimokawa79, Silvestro88] , 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase [Ray80a] , methylglyoxal reductase (NADPH-dependent) [Saikusa87] , allantoinase [Kim00a] , hydrogenase [Ballantine86] , acetylornithine deacetylase [Vogel74a] , glutamate-5-semialdehyde dehydrogenase [Hayzer82] , D-glucuronate isomerase [Ashwell60] , D-galacturonate isomerase [Ashwell60] , glyoxylate carboligase [Gupta66] , acetylornithine aminotransferase [Vogel74b] , N-acetylneuraminate lyase [Aisaka91, Uchida84] , dipeptidyl carboxypeptidase [Henrich93] , threonine dehydrogenase [Boylan81, Craig86] , tetrahydrodipicolinate succinylase [Simms84] , amylomaltase [Wiesmeyer60] , polyphosphate kinase [Haeusler92] , glutamate decarboxylase A [Helmward89] , N-acetylglutamate synthase [Marvil77] , lactaldehyde dehydrogenase [Sridhara69, Baldoma87] , glycerol dehydrogenase [Tang79] , lipoyl-adenylate--protein ligase [Green95] , taurine dioxygenase [Eichhorn97] , 2',3'-cyclic nucleotide 2'-phosphodiesterase [Anraku64] , D-aminopropanol dehydrogenase [Kelley84, Campbell78] , 2-amino-3-ketobutyrate CoA ligase [Mukherjee87]

This compound has been characterized as a cofactor or prosthetic group of the following enzymes: cytochrome bo terminal oxidase , phenylethylamine oxidase , amine oxidase , examinopeptidase , superoxide dismutase

In Growth Media: MOPS medium with 2% glycerol , MOPS medium with 2% glucose , MOPS medium with 0.4% glucose , MOPS medium base , Neidhardt EZ rich defined medium , trace metal solution , LB enriched


References

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Anraku64: Anraku Y (1964). "A new cyclic phosphodiesterase having a 3'-nucleotidase activity from Escherichia coli B. I. Purification and some properties of the enzyme." J Biol Chem 1964;239(10):3412-3419. PMID: 14245396

Ashwell60: Ashwell G, Wahba AJ, Hickman J (1960). "Uronic acid metabolism in bacteria. I. Purification and properties of uronic acid isomerase in Escherichia coli." J Biol Chem 1960;235(6):1559-1565. PMID: 13794771

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Ballantine86: Ballantine SP, Boxer DH (1986). "Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli." Eur J Biochem 1986;156(2);277-84. PMID: 3516690

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HammerJespersen70: Hammer-Jespersen K, Munch-Petersen A (1970). "Phosphodeoxyribomutase from Escherichia coli. Purification and some properties." Eur J Biochem 1970;17(3);397-407. PMID: 4992818

Hartman68: Hartman SC (1968). "Glutaminase of Escherichia coli. I. Purification and general catalytic properties." J Biol Chem 1968;243(5);853-63. PMID: 4966660

Hassaninasab11: Hassaninasab A, Hashimoto Y, Tomita-Yokotani K, Kobayashi M (2011). "Discovery of the curcumin metabolic pathway involving a unique enzyme in an intestinal microorganism." Proc Natl Acad Sci U S A 108(16):6615-20. PMID: 21467222

Hayzer82: Hayzer DJ, Leisinger T (1982). "Proline biosynthesis in Escherichia coli. Purification and characterisation of glutamate-semialdehyde dehydrogenase." Eur J Biochem 1982;121(3);561-5. PMID: 7035170

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Henrich93: Henrich B, Becker S, Schroeder U, Plapp R (1993). "dcp gene of Escherichia coli: cloning, sequencing, transcript mapping, and characterization of the gene product." J Bacteriol 175(22);7290-300. PMID: 8226676

Itoh08: Itoh T, Mikami B, Hashimoto W, Murata K (2008). "Crystal structure of YihS in complex with D-mannose: structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase." J Mol Biol 377(5);1443-59. PMID: 18328504

Kelley84: Kelley JJ, Dekker EE (1984). "D-1-amino-2-propanol:NAD+ oxidoreductase. Purification and general properties of the large molecular form of the enzyme from Escherichia coli K12." J Biol Chem 1984;259(4);2124-9. PMID: 6365902

Kim00a: Kim GJ, Lee DE, Kim HS (2000). "Functional expression and characterization of the two cyclic amidohydrolase enzymes, allantoinase and a novel phenylhydantoinase, from Escherichia coli." J Bacteriol 2000;182(24);7021-8. PMID: 11092864

Marvil77: Marvil DK, Leisinger T (1977). "N-acetylglutamate synthase of Escherichia coli: purification, characterization, and molecular properties." J Biol Chem 1977;252(10);3295-303. PMID: 16890

Mori95: Mori H, Iida A, Teshiba S, Fujio T (1995). "Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product." J Bacteriol 177(17);4921-6. PMID: 7665468

Mukherjee87: Mukherjee JJ, Dekker EE (1987). "Purification, properties, and N-terminal amino acid sequence of homogeneous Escherichia coli 2-amino-3-ketobutyrate CoA ligase, a pyridoxal phosphate-dependent enzyme." J Biol Chem 1987;262(30);14441-7. PMID: 3117785

Newman80a: Newman EB, Kapoor V (1980). "In vitro studies on L-serine deaminase activity of Escherichia coli K12." Can J Biochem 1980;58(11);1292-7. PMID: 7011505

Porter93: Porter DJ, Austin EA (1993). "Cytosine deaminase. The roles of divalent metal ions in catalysis." J Biol Chem 1993;268(32);24005-11. PMID: 8226944

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Roth94: Roth S, Jung K, Jung H, Hommel RK, Kleber HP (1994). "Crotonobetaine reductase from Escherichia coli--a new inducible enzyme of anaerobic metabolization of L(-)-carnitine." Antonie Van Leeuwenhoek 1994;65(1);63-9. PMID: 8060125

Saikusa87: Saikusa T, Rhee H, Watanabe K, Murata K, Kimura A (1987). "Metabolism of 2-oxoaldehydes in bacteria: purification and characterization of methylglyoxal reductase from E. coli." Agricultural and Biological Chemistry 51(7): 1893-1899.

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Simms84: Simms SA, Voige WH, Gilvarg C (1984). "Purification and characterization of succinyl-CoA: tetrahydrodipicolinate N-succinyltransferase from Escherichia coli." J Biol Chem 1984;259(5);2734-41. PMID: 6365916

Sridhara69: Sridhara S, Wu TT (1969). "Purification and properties of lactaldehyde dehydrogenase from Escherichia coli." J Biol Chem 1969;244(19);5233-8. PMID: 4310089

Subedi11: Subedi KP, Choi D, Kim I, Min B, Park C (2011). "Hsp31 of Escherichia coli K-12 is glyoxalase III." Mol Microbiol 81(4);926-36. PMID: 21696459

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Uchida84: Uchida Y, Tsukada Y, Sugimori T (1984). "Purification and properties of N-acetylneuraminate lyase from Escherichia coli." J Biochem 96(2);507-22. PMID: 6389524

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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