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Escherichia coli K-12 substr. MG1655 Compound: Mn2+

Synonyms: manganese ion, Mn(II), Mn+2, Mn++, manganese (II) ion

Superclasses: an ion a cation

Component of:
manganese chloride
manganese sulfate pentahydrate
MnSO4
MntR-Mn2+

Chemical Formula: Mn

Molecular Weight: 54.938 Daltons

Monoisotopic Molecular Weight: 54.9380496 Daltons

SMILES: [Mn++]

InChI: InChI=1S/Mn/q+2

InChIKey: InChIKey=WAEMQWOKJMHJLA-UHFFFAOYSA-N

Unification Links: CAS:7439-96-5 , CAS:16397-91-4 , ChEBI:29035 , ChemSpider:25916 , HMDB:HMDB01333 , IAF1260:33594 , KEGG:C19610 , PubChem:27854

Standard Gibbs Free Energy of Change Formation (ΔfG in kcal/mol): 0.0

In Reactions of unknown directionality:

Not in pathways:
MntR + Mn2+ = MntR-Mn2+

In Transport reactions:
Mn2+[periplasmic space]Mn2+[cytosol] ,
Mn2+[cytosol]Mn2+[periplasmic space] ,
Mn2+[periplasmic space] + H+[periplasmic space]Mn2+[cytosol] + H+[cytosol]

Enzymes activated by Mn2+, sorted by the type of activation, are:

Activator (Mechanism unknown) of: cysteinylglycine dipeptidase [Suzuki01] , adenosylmethionine decarboxylase [Lu07] , cyclic di-GMP phosphodiesterase [Lacey10] , D-altronate dehydratase [Dreyer87] , glutaminase B [Prusiner76] , D-mannonate oxidoreductase [Portalier72] , D-mannonate dehydratase [RobertBaudouy73, Dreyer87] , threonine dehydrogenase [Comment 1] , polyphosphate kinase [Ahn90, Haeusler92, Comment 2] , trimethylamine N-oxide reductase [Sagai73] , proline aminopeptidase [Yoshimoto89]

Enzymes inhibited by Mn2+, sorted by the type of inhibition, are:

Inhibitor (Mechanism unknown) of: biotin sulfoxide reductase [Del79] , methylmalonyl-CoA mutase [Bobik03] , glutaminase [Brown08] , glutaminase [Brown08] , diacylglycerol pyrophosphate phosphatase [Dillon96] , N-acetylglutamylphosphate reductase [Vogel74] , imidazole glycerol phosphate synthase [Klem93] , phosphoglucomutase [Joshi64] , Rnase [Goedken01] , D-glucarate dehydratase [Comment 3] , D-aminopropanol dehydrogenase [Kelley84, Campbell78]

This compound has been characterized as a cofactor or prosthetic group of the following enzymes: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase , cytidine 3'-phosphate phosphohydrolase , dGMP 5'-phosphate phosphohydrolase , adenosine 3'-phosphate phosphohydrolase , UDP-N-acetylmuramate-alanine ligase , monoacetylchitobiose-6-phosphate hydrolase , 6-phospho-β-D-glucosyl-(1,4)-D-glucose glucohydrolase , phosphoribosylglycinamide formyltransferase , 5'-nucleotidase , 3'-nucleotidase , 8-oxo-dGTP diphosphatase , 2-oxopent-4-enoate hydratase , transketolase , 5-phospho-α-D-ribosyl 1,2-cyclic phosphate phosphodiesterase , 2,3-bisphosphoglycerate-independent phosphoglycerate mutase , RNA ligase , L-ascorbate 6-phosphate lactonase , p-aminobenzoyl-glutamate hydrolase , S-ureidoglycine aminohydrolase , allantoate amidohydrolase , coproporphyrinogen III oxidase , diacylglycerol kinase , tRNA nucleotidyltransferase , fructose-1,6-bisphosphatase , peptidoglycan glycosyltransferase , agmatinase , oligoribonuclease , protein kinase , L-arabinose isomerase , L-fucose isomerase , D-arabinose isomerase , ThDP adenylyl transferase , histidinol dehydrogenase , histidinal dehydrogenase , glutamine synthetase , 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase , 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase , xylose isomerase , 2-deoxyglucose-6-phosphatase , 3-isopropylmalate dehydrogenase , aminopeptidase , examinopeptidase , proline aminopeptidase , deoxyribose 1,5-phosphomutase , adenine deaminase , L-rhamnose isomerase , 1-deoxy-D-xylulose 5-phosphate reductoisomerase , α-D-ribose 1,5-phosphomutase , pyrimidine nucleotidase , guanylate cyclase , glutamine synthetase deadenylase , transketolase , malate dehydrogenase, NAD-requiring , malate dehydrogenase , ribonuclease , 3-octaprenyl-4-hydroxybenzoate carboxy-lyase , RNase T exonuclease , 2-keto-3-deoxygluconokinase , Rnase , phosphoglycerol transferase , phenylhydantoinase , superoxide dismutase , guanosine-3',5'-bis(diphosphate) 3'-diphosphatase , acyl carrier protein phosphodiesterase , NADH pyrophosphatase , phosphoglycerol transferase , isocitrate dehydrogenase , phosphodiesterase, c-di-GMP-specific , RNase , fructose 1,6-bisphosphatase , RNA 3'-terminal phosphate cyclase , α-galactosidase , 3' to 5' proofreading exonuclease

In Growth Media: MOPS medium with 2% glycerol , MOPS medium with 2% glucose , MOPS medium with 0.4% glucose , MOPS medium base , Neidhardt EZ rich defined medium , trace metal solution , LB enriched


References

Ahn90: Ahn K, Kornberg A (1990). "Polyphosphate kinase from Escherichia coli. Purification and demonstration of a phosphoenzyme intermediate." J Biol Chem 1990;265(20);11734-9. PMID: 2164013

Blumenthal66: Blumenthal H "D-Glucarate Dehydrase." Meth Enz 1966;9:660-665.

Bobik03: Bobik TA, Rasche ME (2003). "HPLC assay for methylmalonyl-CoA epimerase." Anal Bioanal Chem 375(3);344-9. PMID: 12589497

Brown08: Brown G, Singer A, Proudfoot M, Skarina T, Kim Y, Chang C, Dementieva I, Kuznetsova E, Gonzalez CF, Joachimiak A, Savchenko A, Yakunin AF (2008). "Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis." Biochemistry 47(21);5724-35. PMID: 18459799

Campbell78: Campbell RL, Swain RR, Dekker EE (1978). "Purification, separation, and characterization of two molecular forms of D-1-amino-2-propanol:NAD+ oxidoreductase activity from extracts of Escherichia coli K-12." J Biol Chem 253(20);7282-8. PMID: 359547

Craig86: Craig PA, Dekker EE (1986). "L-threonine dehydrogenase from Escherichia coli K-12: thiol-dependent activation by Mn2+." Biochemistry 1986;25(8);1870-6. PMID: 3518793

Del79: Del Campillo-Campbell, A, Dykhuizen, D, Cleary, PP "Enzymatic reduction of d-biotin d-sulfoxide to d-biotin." Methods in Enzymology 62:379-385 (1979).

Dillon96: Dillon DA, Wu WI, Riedel B, Wissing JB, Dowhan W, Carman GM (1996). "The Escherichia coli pgpB gene encodes for a diacylglycerol pyrophosphate phosphatase activity." J Biol Chem 271(48);30548-53. PMID: 8940025

Dreyer87: Dreyer JL (1987). "The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases." Eur J Biochem 166(3);623-30. PMID: 3038546

Goedken01: Goedken ER, Marqusee S (2001). "Co-crystal of Escherichia coli RNase HI with Mn2+ ions reveals two divalent metals bound in the active site." J Biol Chem 276(10);7266-71. PMID: 11083878

Haeusler92: Haeusler PA, Dieter L, Rittle KJ, Shepler LS, Paszkowski AL, Moe OA (1992). "Catalytic properties of Escherichia coli polyphosphate kinase: an enzyme for ATP regeneration." Biotechnol Appl Biochem 1992;15(2);125-33. PMID: 1316760

Joshi64: Joshi JG, Handler P (1964). "Phosphoglucomutase. I. Purification and properties of phosphoglucomutase from Escherichia coli." J. Biol. Chem. 239:2741-2751. PMID: 14216423

Kelley84: Kelley JJ, Dekker EE (1984). "D-1-amino-2-propanol:NAD+ oxidoreductase. Purification and general properties of the large molecular form of the enzyme from Escherichia coli K12." J Biol Chem 1984;259(4);2124-9. PMID: 6365902

Klem93: Klem TJ, Davisson VJ (1993). "Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis." Biochemistry 1993;32(19);5177-86. PMID: 8494895

Lacey10: Lacey MM, Partridge JD, Green J (2010). "Escherichia coli K-12 YfgF is an anaerobic cyclic di-GMP phosphodiesterase with roles in cell surface remodelling and the oxidative stress response." Microbiology 156(Pt 9);2873-86. PMID: 20522491

Lu07: Lu ZJ, Markham GD (2007). "Metal ion activation of S-adenosylmethionine decarboxylase reflects cation charge density." Biochemistry 46(27);8172-80. PMID: 17567041

Portalier72: Portalier RC, Stoeber FR (1972). "[D-mannonate: NAD oxidoreductase from Escherichia coli K12. Purification, properties and specificity]." Eur J Biochem 1972;26(2);290-300. PMID: 4402996

Prusiner76: Prusiner S, Stadtman ER (1976). "Regulation of glutaminase B in Escherichia coli. III. Control by nucleotides and divalent cations." J Biol Chem 1976;251(11);3463-9. PMID: 776970

RobertBaudouy73: Robert-Baudouy JM, Stoeber FR (1973). "[Purification and properties of D-mannonate hydrolyase from Escherichia coli K12]." Biochim Biophys Acta 1973;309(2);473-85. PMID: 4581499

Sagai73: Sagai M, Ishimoto M (1973). "An enzyme reducing adenosine 1N-oxide in Escherichia coli, amine N-oxide reductase." J Biochem (Tokyo) 1973;73(4);843-59. PMID: 4578389

Suzuki01: Suzuki H, Kamatani S, Kumagai H (2001). "Purification and characterization of aminopeptidase B from Escherichia coli K-12." Biosci Biotechnol Biochem 65(7);1549-58. PMID: 11515538

Vogel74: Vogel HJ, Vogel RH (1974). "Enzymes of arginine biosynthesis and their repressive control." Adv Enzymol Relat Areas Mol Biol 1974;40(0);65-90. PMID: 4365537

Yoshimoto89: Yoshimoto T, Tone H, Honda T, Osatomi K, Kobayashi R, Tsuru D (1989). "Sequencing and high expression of aminopeptidase P gene from Escherichia coli HB101." J Biochem (Tokyo) 105(3);412-6. PMID: 2659585


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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