Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: 1-phosphofructokinase



Gene: fruK Accession Numbers: EG10337 (EcoCyc), b2168, ECK2161

Synonyms: fpk, fruF

Regulation Summary Diagram: ?

Subunit composition of 1-phosphofructokinase = [FruK]2
         1-phosphofructokinase monomer = FruK

Summary:
1-phosphofructokinase is essential for the utilization of fructose as a carbon source. The enzyme is highly specific for fructose-1-phosphate [Buschmeier85].

fruK has some sequence similarity to the minor form of 6-phosphofructokinase (pfkB), but not the major form (pfkA) [Orchard90]. FruK belongs to the ribokinase family of sugar kinases [Bork93].

A fruK mutant is unable to grow on fructose or fructose-1-phosphate as the sole source of carbon [Ferenci73].

Review: [Kornberg01]

Gene Citations: [Reizer94, Dobrynina95]

Locations: cytosol

Map Position: [2,259,449 <- 2,260,387] (48.7 centisomes)
Length: 939 bp / 312 aa

Molecular Weight of Polypeptide: 33.756 kD (from nucleotide sequence), 34.0 kD (experimental) [VeigadaCunha00 ]

Molecular Weight of Multimer: 57.0 kD (experimental) [Buschmeier85]

Unification Links: ASAP:ABE-0007174 , CGSC:743 , EchoBASE:EB0333 , EcoGene:EG10337 , EcoliWiki:b2168 , ModBase:P0AEW9 , OU-Microarray:b2168 , PortEco:fruK , PR:PRO_000022701 , Pride:P0AEW9 , Protein Model Portal:P0AEW9 , RefSeq:NP_416673 , RegulonDB:EG10337 , SMR:P0AEW9 , String:511145.b2168 , UniProt:P0AEW9

Relationship Links: InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR017583 , InterPro:IN-FAMILY:IPR022463 , Pfam:IN-FAMILY:PF00294 , Prosite:IN-FAMILY:PS00583 , Prosite:IN-FAMILY:PS00584

In Paralogous Gene Group: 340 (11 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006001 - fructose catabolic process Inferred from experiment [Ferenci73]
GO:0046835 - carbohydrate phosphorylation Inferred by computational analysis Inferred from experiment [VeigadaCunha00, GOA01, GOA01a]
GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0008662 - 1-phosphofructokinase activity Inferred from experiment Inferred by computational analysis [GOA01, GOA01a, VeigadaCunha00]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016773 - phosphotransferase activity, alcohol group as acceptor Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism energy metabolism, carbon

Essentiality data for fruK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 16-Aug-2007 by Keseler I , SRI International


Enzymatic reaction of: 1-phosphofructokinase

Synonyms: phosphofructokinase, fructose-1-phosphate kinase

EC Number: 2.7.1.56

ATP + β-D-fructofuranose 1-phosphate <=> ADP + fructose 1,6-bisphosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for ATP: GTP [Buschmeier85 ]

In Pathways: fructose degradation

Summary:
In the presence of 50 mM KCl, the Km for fructose-1-phosphate decreases to 125 µM, and the Km for ATP increases to 600 µM. Half-maximal activation of the enzyme is obtained with 4 mM KCl. The Km of the enzyme for fructose-6-phosphate is 3.5 mM [VeigadaCunha00].

Cofactors or Prosthetic Groups: Mg2+ [Buschmeier85]

Activators (Unknown Mechanism): potassium chloride [VeigadaCunha00]

Inhibitors (Competitive): ADP [Buschmeier85]

Inhibitors (Irreversible): phenylmethanesulfonyl fluoride [Buschmeier85]

Inhibitors (Unknown Mechanism): fructose 1,6-bisphosphate [Buschmeier85]

Kinetic Parameters:

Substrate
Km (μM)
Citations
β-D-fructofuranose 1-phosphate
360.0
[VeigadaCunha00]
ATP
600.0, 350.0
[VeigadaCunha00, BRENDA14]

pH(opt): 7.8 [Buschmeier85]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2168 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10337; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bork93: Bork P, Sander C, Valencia A (1993). "Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases." Protein Sci 2(1);31-40. PMID: 8382990

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Buschmeier85: Buschmeier B, Hengstenberg W, Deutscher J (1985). "Purification and properties of 1-phosphofructokinase from Escherichia coli." FEMS Microbiol Lett 1985;29(3):231-235.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dobrynina95: Dobrynina OIu, Bol'shakova TN, Umiarov AM, Gershanovich VN (1995). "[Determination of the direction of transcription of Escherichia coli K-12 structural genes of the fructose regulon in vivo]." Genetika 31(2);276-7. PMID: 7721070

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Ferenci73: Ferenci T, Kornberg HL (1973). "The utilization of fructose by Escherichia coli. Properties of a mutant defective in fructose 1-phosphate kinase activity." Biochem J 132(2);341-7. PMID: 4579702

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kornberg01: Kornberg HL (2001). "Routes for fructose utilization by Escherichia coli." J Mol Microbiol Biotechnol 3(3);355-9. PMID: 11361065

Orchard90: Orchard LM, Kornberg HL (1990). "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus." Proc R Soc Lond B Biol Sci 1990;242(1304);87-90. PMID: 1981619

Reizer94: Reizer J, Reizer A, Kornberg HL, Saier MH (1994). "Sequence of the fruB gene of Escherichia coli encoding the diphosphoryl transfer protein (DTP) of the phosphoenolpyruvate: sugar phosphotransferase system." FEMS Microbiol Lett 1994;118(1-2);159-62. PMID: 8013873

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

VeigadaCunha00: Veiga-da-Cunha M, Hoyoux A, Van Schaftingen E, Houyoux A (2000). "Overexpression and purification of fructose-1-phosphate kinase from Escherichia coli: application to the assay of fructose 1-phosphate." Protein Expr Purif 19(1);48-52. PMID: 10833389

Other References Related to Gene Regulation

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Ramseier93: Ramseier TM, Negre D, Cortay JC, Scarabel M, Cozzone AJ, Saier MH (1993). "In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru, pps, ace, pts and icd operons of Escherichia coli and Salmonella typhimurium." J Mol Biol 234(1);28-44. PMID: 8230205

Shimada11: Shimada T, Yamamoto K, Ishihama A (2011). "Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli." J Bacteriol 193(3);649-59. PMID: 21115656


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.