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Escherichia coli K-12 substr. MG1655 Transporter: histidine ABC transporter

Subunit composition of histidine ABC transporter = [HisP]2[HisM][HisQ][HisJ]
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit = HisP
         lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisM
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisQ
         histidine ABC transporter - periplasmic binding protein = HisJ

Summary:
HisPMQJ is an ATP-dependent histidine transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95a]. Based on sequence similarity, HisJ is the periplasmic histidine-binding protein, HisQ and HisM are the integral membrane components, and HisP is the ATP-binding component of the ABC transport complex [Liu98]. Although the transport properties of the His complex in E. coli have not yet been characterized, extensive investigations on the orthologous proteins in Salmonella typhimurium have been reported. The His complex of Salmonella typhimurium was purified and reconstituted into ATP-encapsulated proteoliposomes, and histidine transport activity was observed [Liu97a]. His-mediated transport activity is completely dependent on the presence of all four protein components and on the internal ATP concentration, with apparent Km of 8 mM for ATP [Liu97a, Bishop89]. The transport activity is also affected by pH, temperature, and salt concentration [Liu97a]. Transport is irreversible and accumulation reaches a plateau at which point transport ceases [Liu97a]. The transport complex is inhibited by ADP and by high concentrations of internal histidine [Liu97a].

Gene-Reaction Schematic: ?


Enzymatic reaction of: histidine ABC transporter


Subunit of histidine ABC transporter: lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit

Gene: hisP Accession Numbers: EG10452 (EcoCyc), b2306, ECK2300

Locations: inner membrane

Sequence Length: 257 AAs

Molecular Weight: 28.653 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
GO:0015817 - histidine transport Inferred by computational analysis [Ames70]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids arginine
metabolism biosynthesis of building blocks amino acids histidine
metabolism biosynthesis of building blocks amino acids lysine
metabolism carbon utilization amino acids
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b2306 , ModBase:P07109 , PR:PRO_000022901 , Pride:P07109 , Protein Model Portal:P07109 , RefSeq:NP_416809 , SMR:P07109 , String:511145.b2306 , Swiss-Model:P07109 , UniProt:P07109

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Essentiality data for hisP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of histidine ABC transporter: lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit

Gene: hisM Accession Numbers: EG10007 (EcoCyc), b2307, ECK2301

Locations: inner membrane

Sequence Length: 238 AAs

Molecular Weight: 26.87 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids arginine
metabolism biosynthesis of building blocks amino acids lysine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b2307 , PR:PRO_000022900 , Protein Model Portal:P0AEU3 , RefSeq:NP_416810 , String:511145.b2307 , UniProt:P0AEU3

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Essentiality data for hisM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of histidine ABC transporter: lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit

Synonyms: HisQ

Gene: hisQ Accession Numbers: EG12125 (EcoCyc), b2308, ECK2302

Locations: inner membrane

Sequence Length: 228 AAs

Molecular Weight: 24.649 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids arginine
metabolism biosynthesis of building blocks amino acids lysine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: DIP:DIP-9912N , EcoliWiki:b2308 , PR:PRO_000022902 , Pride:P52094 , Protein Model Portal:P52094 , RefSeq:NP_416811 , String:511145.b2308 , UniProt:P52094

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Essentiality data for hisQ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of histidine ABC transporter: histidine ABC transporter - periplasmic binding protein

Gene: hisJ Accession Numbers: EG12124 (EcoCyc), b2309, ECK2303

Locations: periplasmic space

Sequence Length: 260 AAs

Molecular Weight: 28.483 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [GOA01, DiazMejia09, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids histidine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b2309 , ModBase:P0AEU0 , PR:PRO_000022899 , Pride:P0AEU0 , Protein Model Portal:P0AEU0 , RefSeq:NP_416812 , SMR:P0AEU0 , String:511145.b2309 , Swiss-Model:P0AEU0 , UniProt:P0AEU0

Relationship Links: InterPro:IN-FAMILY:IPR001638 , InterPro:IN-FAMILY:IPR005768 , InterPro:IN-FAMILY:IPR018313 , PDB:Structure:1HSL , Pfam:IN-FAMILY:PF00497 , Prosite:IN-FAMILY:PS01039 , Smart:IN-FAMILY:SM00062

Essentiality data for hisJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Ames70: Ames GF, Lever J (1970). "Components of histidine transport: histidine-binding proteins and hisP protein." Proc Natl Acad Sci U S A 66(4);1096-103. PMID: 4920090

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop89: Bishop L, Agbayani R, Ambudkar SV, Maloney PC, Ames GF (1989). "Reconstitution of a bacterial periplasmic permease in proteoliposomes and demonstration of ATP hydrolysis concomitant with transport." Proc Natl Acad Sci U S A 1989;86(18);6953-7. PMID: 2674940

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Liu97a: Liu CE, Ames GF (1997). "Characterization of transport through the periplasmic histidine permease using proteoliposomes reconstituted by dialysis." J Biol Chem 1997;272(2);859-66. PMID: 8995374

Liu98: Liu PQ, Ames GF (1998). "In vitro disassembly and reassembly of an ABC transporter, the histidine permease." Proc Natl Acad Sci U S A 1998;95(7);3495-500. PMID: 9520394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95a: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13A.