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Escherichia coli K-12 substr. MG1655 Transporter: methyl-β-D-galactoside / galactose ABC transporter

Synonyms: MglBAC

Subunit composition of methyl-β-D-galactoside / galactose ABC transporter = [MglA]2[MglC]2[MglB]
         galactose ABC transporter - ATP binding subunit = MglA (summary available)
         galactose ABC transporter - membrane subunit = MglC (summary available)
         galactose ABC transporter - periplasmic binding protein = MglB (summary available)

Summary:
MglBAC is a beta-methylgalactoside transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, mglB encodes the galactose-binding component, mglC encodes the integral membrane component, and mglA encodes the ATP-binding component of the ABC transporter. Insertional mutations in each gene indicate that all three components are necessary for beta-methylgalactoside transport function [Harayama83]. Complementation experiments show that mlg genes cloned into a plasmid vector are able to complement the transport functions of the mglA, mglB, and mglC mutants [Rotman82]. MglB, but not MglA or MglC, was found to also serve as the galactose chemoreceptor in E. coli [Harayama83]. mglB mutants eliminate the chemotactic function in E. coli; however, mglA and MglC mutants exhibit normal galactose taxis but defective galactoside uptake activities [Scholle87, Harayama83].

Gene-Reaction Schematic: ?


Enzymatic reaction of: transport of methyl-β-D-galactoside (methyl-β-D-galactoside / galactose ABC transporter)


Enzymatic reaction of: transport of β-D-galactose (methyl-β-D-galactoside / galactose ABC transporter)

EC Number: 3.6.3.17


Subunit of methyl-β-D-galactoside / galactose ABC transporter: galactose ABC transporter - ATP binding subunit

Synonyms: MglP, Mgl, MglA

Gene: mglA Accession Numbers: EG10592 (EcoCyc), b2149, ECK2142

Locations: inner membrane, cytosol

Sequence Length: 506 AAs

Molecular Weight: 56.415 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0015757 - galactose transport Inferred from experiment [Ordal74]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11, GOA06]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0005354 - galactose transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, Ordal74]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA06, GOA01]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
GO:0043211 - carbohydrate-transporting ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Harayama83]
GO:0005829 - cytosol
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA06]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b2149 , ModBase:P0AAG8 , PR:PRO_000023222 , Pride:P0AAG8 , Protein Model Portal:P0AAG8 , RefSeq:NP_416654 , SMR:P0AAG8 , String:511145.b2149 , UniProt:P0AAG8

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR015862 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51260 , Smart:IN-FAMILY:SM00382

Summary:
MglA is the ATP-binding component of the galactose ABC transporter.

Essentiality data for mglA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of methyl-β-D-galactoside / galactose ABC transporter: galactose ABC transporter - membrane subunit

Synonyms: MglP, MglC

Gene: mglC Accession Numbers: EG10594 (EcoCyc), b2148, ECK2141

Locations: inner membrane

Sequence Length: 336 AAs

Molecular Weight: 35.55 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b2148 , PR:PRO_000023224 , Pride:P23200 , Protein Model Portal:P23200 , RefSeq:NP_416653 , String:511145.b2148 , UniProt:P23200

Relationship Links: InterPro:IN-FAMILY:IPR001851 , Pfam:IN-FAMILY:PF02653

Summary:
MglC is the integral membrane component of the galactose ABC transporter.

Essentiality data for mglC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of methyl-β-D-galactoside / galactose ABC transporter: galactose ABC transporter - periplasmic binding protein

Synonyms: MglB

Gene: mglB Accession Numbers: EG10593 (EcoCyc), b2150, ECK2143

Locations: periplasmic space

Sequence Length: 332 AAs

Molecular Weight: 35.713 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006935 - chemotaxis Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Zheng11]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Han13, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: information transfer protein related chaperoning, repair (refolding)
metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: DIP:DIP-35953N , EcoliWiki:b2150 , Mint:MINT-1274707 , ModBase:P0AEE5 , PR:PRO_000023223 , Pride:P0AEE5 , Protein Model Portal:P0AEE5 , RefSeq:NP_416655 , SMR:P0AEE5 , String:511145.b2150 , Swiss-Model:P0AEE5 , UniProt:P0AEE5

Relationship Links: InterPro:IN-FAMILY:IPR025997 , InterPro:IN-FAMILY:IPR028082 , PDB:Structure:1GLG , PDB:Structure:2FVY , PDB:Structure:2FW0 , PDB:Structure:2GBP , PDB:Structure:2HPH , PDB:Structure:2IPL , PDB:Structure:2IPM , PDB:Structure:2IPN , PDB:Structure:2QW1 , Pfam:IN-FAMILY:PF13407

Summary:
MglB is the periplasmic binding component of the galactose ABC transporter.

Essentiality data for mglB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Han13: Han MJ, Kim JY, Kim JA (2013). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng. PMID: 24140104

Harayama83: Harayama S, Bollinger J, Iino T, Hazelbauer GL (1983). "Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning." J Bacteriol 1983;153(1);408-15. PMID: 6294056

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Ordal74: Ordal GW, Adler J (1974). "Properties of mutants in galactose taxis and transport." J Bacteriol 117(2);517-26. PMID: 4359648

Robbins75: Robbins AR, Rotman B (1975). "Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12." Proc Natl Acad Sci U S A 72(2);423-7. PMID: 1091926

Robbins76: Robbins AR, Guzman R, Rotman B (1976). "Roles of individual mgl gene products in the beta-methylgalactoside transport system of Escherichia coli K12." J Biol Chem 251(10);3112-6. PMID: 773938

Rotman82: Rotman B, Guzman R (1982). "Identification of the mglA gene product in the beta-methylgalactoside transport system of Escherichia coli using plasmid DNA deletions generated in vitro." J Biol Chem 1982;257(15);9030-4. PMID: 6807987

Scholle87: Scholle A, Vreemann J, Blank V, Nold A, Boos W, Manson MD (1987). "Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors." Mol Gen Genet 1987;208(1-2);247-53. PMID: 3302609

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Zheng11: Zheng C, Yang L, Hoopmann MR, Eng JK, Tang X, Weisbrod CR, Bruce JE (2011). "Cross-linking measurements of in vivo protein complex topologies." Mol Cell Proteomics 10(10);M110.006841. PMID: 21697552


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.