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Escherichia coli K-12 substr. MG1655 Protein: phospholipid ABC transporter

Subunit composition of phospholipid ABC transporter = [MlaC][MlaF]2[MlaE]2[MlaD][MlaB]2

Summary:
The MlaBCDEF phospholipid transporter is a member of the ATP-binding cassette (ABC) superfamily of transporters [Saurin99, Malinverni09]. Based on sequence similarity MlaC is the periplasmic binding protein, MlaE and MlaD are integral membrane components and MlaF is the ATP binding component. The Mla pathway prevents phospholipid accumulation in the outer leaflet of the outer membrane (OM).

Single deletion mutants in each of the mla genes results in increased OM permeability as measured by sensitivity to SDS-EDTA [Malinverni09]. The defects in membrane permeability of an mlaC mutant could be suppressed by an increase in pldA transcription [Malinverni09]. An mlaC mutant has increased levels of phospholipids in the outer leaflet of the OM [Malinverni09].

The fate of phospholipid is unknown but may be incorporated in to the inner membrane [Malinverni09]

Locations: inner membrane

Gene-Reaction Schematic: ?

GO Terms:

Molecular Function: GO:0005543 - phospholipid binding Inferred from experiment [Malinverni09]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred by computational analysis [Malinverni09]
Cellular Component: GO:0016021 - integral component of membrane Inferred by computational analysis [Malinverni09]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Malinverni09]

Credits:
Last-Curated ? 21-May-2009 by Mackie A , Macquarie University


Subunit of phospholipid ABC transporter: MlaC

Synonyms: YrbC

Gene: mlaC Accession Numbers: G7659 (EcoCyc), b3192, ECK3181

Locations: periplasmic space, inner membrane

Sequence Length: 211 AAs

Molecular Weight: 23.963 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
Molecular Function: GO:0005543 - phospholipid binding Inferred from experiment [Malinverni09]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [Malinverni09]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Malinverni09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b3192 , Pride:P0ADV7 , Protein Model Portal:P0ADV7 , RefSeq:NP_417659 , SMR:P0ADV7 , String:511145.b3192 , UniProt:P0ADV7

Relationship Links: InterPro:IN-FAMILY:IPR008869 , InterPro:IN-FAMILY:IPR023094 , Pfam:IN-FAMILY:PF05494

Summary:
MlaC is predicted to be the periplasmic binding protein of the phospholipid ABC transporter. An mlaC mutant shows increased accumulation of phospholipids (PLs) in the outer leaflet of the outer membrane (OM) [Malinverni09]. This effect could be suppressed by increasing the levels of PldA, an OM phospholipase that destroys surface-exposed PLs [Malinverni09].

MlaC: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: MlaF

Synonyms: YrbF

Gene: mlaF Accession Numbers: EG12801 (EcoCyc), b3195, ECK3184

Locations: cytosol, inner membrane

Sequence Length: 269 AAs

Molecular Weight: 29.097 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Butland05, Arifuzzaman06]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred by curator
Cellular Component: GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred from experiment [Malinverni09]
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: DIP:DIP-35865N , EcoliWiki:b3195 , Mint:MINT-1228070 , ModBase:P63386 , Pride:P63386 , Protein Model Portal:P63386 , RefSeq:NP_417662 , SMR:P63386 , String:511145.b3195 , UniProt:P63386

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Summary:
MlaF is predicted to be the ATP binding component of the phospholipid ABC transporter. An mlaF mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaF: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: MlaE

Synonyms: YrbE

Gene: mlaE Accession Numbers: EG12800 (EcoCyc), b3194, ECK3183

Locations: inner membrane

Sequence Length: 260 AAs

Molecular Weight: 27.863 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005543 - phospholipid binding Inferred from experiment [Malinverni09]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Malinverni09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b3194 , Protein Model Portal:P64606 , RefSeq:NP_417661 , String:511145.b3194 , UniProt:P64606

Relationship Links: InterPro:IN-FAMILY:IPR003453 , Pfam:IN-FAMILY:PF02405

Summary:
MlaE is an integral membrane component of the phospholipid ABC transporter. An mlaE mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaE: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: MlaD

Synonyms: YrbD

Gene: mlaD Accession Numbers: EG12799 (EcoCyc), b3193, ECK3182

Locations: inner membrane

Sequence Length: 183 AAs

Molecular Weight: 19.576 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005543 - phospholipid binding Inferred from experiment [Malinverni09]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Malinverni09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]
GO:0031226 - intrinsic component of plasma membrane Inferred by computational analysis
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b3193 , Mint:MINT-6478352 , Pride:P64604 , Protein Model Portal:P64604 , RefSeq:NP_417660 , String:511145.b3193 , UniProt:P64604

Relationship Links: InterPro:IN-FAMILY:IPR003399 , Pfam:IN-FAMILY:PF02470

Summary:
MlaD is predicted to be the substrate binding protein of the phospholipid ABC transporter. An mlaD mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaD: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: MlaB

Synonyms: YrbB

Gene: mlaB Accession Numbers: G7658 (EcoCyc), b3191, ECK3180

Locations: cytosol, inner membrane

Sequence Length: 97 AAs

Molecular Weight: 10.68 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Han10c]
GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
GO:0046677 - response to antibiotic Inferred from experiment [Han10c]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
Cellular Component: GO:0005829 - cytosol Inferred from experiment [Ishihama08, Zhang07]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Malinverni09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component
transport

Unification Links: DIP:DIP-47898N , EcoliWiki:b3191 , ModBase:P64602 , Pride:P64602 , Protein Model Portal:P64602 , RefSeq:NP_417658 , SMR:P64602 , String:511145.b3191 , UniProt:P64602

Relationship Links: InterPro:IN-FAMILY:IPR002645 , Pfam:IN-FAMILY:PF13466 , Prosite:IN-FAMILY:PS50801

Summary:
MlaB is part of the phospholipid ABC transporter. MlaB is a predicted cytoplasmic protein with a STAS domain [Malinverni09]

mlaB was identified in a screen for genes that reduce the lethal effects of stress. An mlaB insertion mutant is more sensitive than wild type to mitomycin C and other stresses [Han10c].

MlaB: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 4]

References

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han10c: Han X, Dorsey-Oresto A, Malik M, Wang JY, Drlica K, Zhao X, Lu T (2010). "Escherichia coli genes that reduce the lethal effects of stress." BMC Microbiol 10;35. PMID: 20128927

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Malinverni09: Malinverni JC, Silhavy TJ (2009). "An ABC transport system that maintains lipid asymmetry in the Gram-negative outer membrane." Proc Natl Acad Sci U S A 106(19):8009-14. PMID: 19383799

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc13.