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Escherichia coli K-12 substr. MG1655 Protein: phospholipid ABC transporter
Inferred from experiment

Superclasses: a protein complex
ABC Proteins

Subunit composition of phospholipid ABC transporter = [MlaC][MlaF]2[MlaE]2[MlaD][MlaB]2
         phospholipid ABC transporter - periplasmic binding protein = MlaC (summary available)
         phospholipid ABC transporter - ATP binding subunit = MlaF (summary available)
         phospholipid ABC transporter - integral membrane subunit = MlaE (summary available)
         phospholipid ABC transporter - substrate binding protein = MlaD (summary available)
         phospholipid ABC transporter - MlaB subunit = MlaB (summary available)

Summary:
The MlaBCDEF phospholipid transporter is a member of the ATP-binding cassette (ABC) superfamily of transporters [Saurin99, Malinverni09]. Based on sequence similarity MlaC is the periplasmic binding protein, MlaE and MlaD are integral membrane components and MlaF is the ATP binding component. The Mla pathway prevents phospholipid accumulation in the outer leaflet of the outer membrane (OM).

Single deletion mutants in each of the mla genes results in increased OM permeability as measured by sensitivity to SDS-EDTA. The defects in membrane permeability of an mlaC mutant could be suppressed by an increase in pldA transcription. An mlaC mutant has increased levels of phospholipids in the outer leaflet of the OM [Malinverni09].

The fate of phospholipid is unknown but may be incorporated in to the inner membrane [Malinverni09]

Locations: inner membrane

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Molecular Function:
Inferred from experimentGO:0005543 - phospholipid binding [Malinverni09]
Inferred from experimentGO:0005548 - phospholipid transporter activity [Malinverni09]
Inferred by computational analysisGO:0043492 - ATPase activity, coupled to movement of substances [Malinverni09]
Cellular Component:
Inferred by computational analysisGO:0016021 - integral component of membrane [Malinverni09]
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex [Malinverni09]

Credits:
Last-Curated 21-May-2009 by Mackie A, Macquarie University


Subunit of phospholipid ABC transporter: phospholipid ABC transporter - periplasmic binding protein

Synonyms: YrbC, MlaC

Gene: mlaC Accession Numbers: G7659 (EcoCyc), b3192, ECK3181

Locations: periplasmic space, inner membrane

Sequence Length: 211 AAs

Molecular Weight: 23.963 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015914 - phospholipid transport [Malinverni09]
Molecular Function:
Inferred from experimentGO:0005543 - phospholipid binding [Malinverni09]
Cellular Component:
Inferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [Malinverni09]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex [Malinverni09]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b3192, Pride:P0ADV7, Protein Model Portal:P0ADV7, RefSeq:NP_417659, SMR:P0ADV7, UniProt:P0ADV7

Relationship Links: InterPro:IN-FAMILY:IPR008869, InterPro:IN-FAMILY:IPR023094, Pfam:IN-FAMILY:PF05494

Summary:
MlaC is predicted to be the periplasmic binding protein of the phospholipid ABC transporter. An mlaC mutant shows increased accumulation of phospholipids (PLs) in the outer leaflet of the outer membrane (OM) [Malinverni09]. This effect could be suppressed by increasing the levels of PldA, an OM phospholipase that destroys surface-exposed PLs [Malinverni09].

MlaC: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaC knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: phospholipid ABC transporter - ATP binding subunit

Synonyms: YrbF, MlaF

Gene: mlaF Accession Numbers: EG12801 (EcoCyc), b3195, ECK3184

Locations: cytosol, inner membrane

Sequence Length: 269 AAs

Molecular Weight: 29.097 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015914 - phospholipid transport [Malinverni09]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [UniProtGOA11a, GOA01a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Butland05, Arifuzzaman06]
Inferred from experimentGO:0005548 - phospholipid transporter activity [Malinverni09]
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016787 - hydrolase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Inferred by curatorGO:0043492 - ATPase activity, coupled to movement of substances []
Cellular Component:
Inferred from experimentGO:0043190 - ATP-binding cassette (ABC) transporter complex [Malinverni09]
GO:0005737 - cytoplasm []
GO:0005829 - cytosol []
Inferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Unification Links: DIP:DIP-35865N, EcoliWiki:b3195, Mint:MINT-1228070, ModBase:P63386, Pride:P63386, Protein Model Portal:P63386, RefSeq:NP_417662, SMR:P63386, UniProt:P63386

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF00005, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

Summary:
MlaF is predicted to be the ATP binding component of the phospholipid ABC transporter. An mlaF mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaF: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaF knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: phospholipid ABC transporter - integral membrane subunit

Synonyms: YrbE, MlaE

Gene: mlaE Accession Numbers: EG12800 (EcoCyc), b3194, ECK3183

Locations: inner membrane

Sequence Length: 260 AAs

Molecular Weight: 27.863 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015914 - phospholipid transport [Malinverni09]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Molecular Function:
Inferred from experimentGO:0005543 - phospholipid binding [Malinverni09]
Inferred from experimentGO:0005548 - phospholipid transporter activity [Malinverni09]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex [GOA01a, Malinverni09]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Unification Links: EcoliWiki:b3194, Protein Model Portal:P64606, RefSeq:NP_417661, UniProt:P64606

Relationship Links: InterPro:IN-FAMILY:IPR003453, InterPro:IN-FAMILY:IPR030802, Panther:IN-FAMILY:PTHR30188, Pfam:IN-FAMILY:PF02405

Summary:
MlaE is an integral membrane component of the phospholipid ABC transporter. An mlaE mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaE: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaE knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: phospholipid ABC transporter - substrate binding protein

Synonyms: YrbD, MlaD

Gene: mlaD Accession Numbers: EG12799 (EcoCyc), b3193, ECK3182

Locations: inner membrane

Sequence Length: 183 AAs

Molecular Weight: 19.576 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0015914 - phospholipid transport [GOA01a]
Molecular Function:
Inferred from experimentGO:0005543 - phospholipid binding [Malinverni09]
Inferred from experimentGO:0005548 - phospholipid transporter activity [Malinverni09]
Cellular Component:
Inferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, Malinverni09]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]
Inferred by computational analysisGO:0031226 - intrinsic component of plasma membrane []
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex []

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Unification Links: DIP:DIP-12906N, EcoliWiki:b3193, Mint:MINT-6478352, Pride:P64604, Protein Model Portal:P64604, RefSeq:NP_417660, UniProt:P64604

Relationship Links: InterPro:IN-FAMILY:IPR003399, InterPro:IN-FAMILY:IPR030970, Pfam:IN-FAMILY:PF02470

Summary:
MlaD is predicted to be the substrate binding protein of the phospholipid ABC transporter. An mlaD mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaD: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaD knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of phospholipid ABC transporter: phospholipid ABC transporter - MlaB subunit

Synonyms: YrbB, MlaB

Gene: mlaB Accession Numbers: G7658 (EcoCyc), b3191, ECK3180

Locations: cytosol, inner membrane

Sequence Length: 97 AAs

Molecular Weight: 10.68 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0006974 - cellular response to DNA damage stimulus [Han10]
Inferred from experimentGO:0015914 - phospholipid transport [Malinverni09]
Inferred from experimentGO:0046677 - response to antibiotic [Han10]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0005548 - phospholipid transporter activity [Malinverni09]
Cellular Component:
Inferred from experimentGO:0005829 - cytosol [Ishihama08, Zhang07]
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a]
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex [Malinverni09]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component
transport

Unification Links: DIP:DIP-47898N, EcoliWiki:b3191, ModBase:P64602, Pride:P64602, Protein Model Portal:P64602, RefSeq:NP_417658, SMR:P64602, UniProt:P64602

Relationship Links: InterPro:IN-FAMILY:IPR002645, Pfam:IN-FAMILY:PF13466, Prosite:IN-FAMILY:PS50801

Summary:
MlaB is part of the phospholipid ABC transporter. MlaB is a predicted cytoplasmic protein with a STAS domain [Malinverni09]

mlaB was identified in a screen for genes that reduce the lethal effects of stress. An mlaB insertion mutant is more sensitive than wild type to mitomycin C and other stresses [Han10].

MlaB: "outer membrane lipid asymmetry" [Malinverni09]

Essentiality data for mlaB knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxNo 37 Aerobic 7   No [Baba06, Comment 4]

References

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han10: Han X, Dorsey-Oresto A, Malik M, Wang JY, Drlica K, Zhao X, Lu T (2010). "Escherichia coli genes that reduce the lethal effects of stress." BMC Microbiol 10;35. PMID: 20128927

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Malinverni09: Malinverni JC, Silhavy TJ (2009). "An ABC transport system that maintains lipid asymmetry in the Gram-negative outer membrane." Proc Natl Acad Sci U S A 106(19):8009-14. PMID: 19383799

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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