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Escherichia coli K-12 substr. MG1655 Transporter: ferric dicitrate ABC transporter

Synonyms: iron dicitrate ABC transporter, ferric citrate ABC transporter


Subunit composition of ferric dicitrate ABC transporter = [FecE]2[FecC][FecD][FecB]
         ferric dicitrate ABC transporter - ATP binding subunit = FecE (summary available)
         ferric dicitrate ABC transporter - membrane subunit = FecC (summary available)
         ferric dicitrate ABC transporter - membrane subunit = FecD (summary available)
         ferric dicitrate ABC transporter - periplasmic binding protein = FecB (summary available)

Component of: ferric dicitrate transport system

Summary:
FecBCDE is an ATP Binding Cassette (ABC) citrate-dependent iron (III) transport system. Sequence homology and hydropathy analyses indicate that FecB is the periplasmic binding protein, FecC and FecD are integral membrane proteins and FecE is the ATP-binding protein [Pressler88]. Mutation and induction studies indicate that exogenous ferric citrate induces the expression of fec transport genes through a signaling mechanism which does not require ferric citrate to enter the cytoplasm [Hussein81]. [Wagegg81], or to cross the outer membrane into the periplasmic space [Harle95]. Rather, induction of fec transport genes is a function of FecA-ferric citrate binding and is coupled through the TonB, ExbB and ExbD proteins independent of their role in uptake [Harle95].

Gene-Reaction Schematic

Gene-Reaction Schematic


Enzymatic reaction of: transport of ferric dicitrate (ferric dicitrate ABC transporter)

Inferred from experimentInferred by computational analysis

EC Number: 3.6.3.34

Transport reaction diagram for transport of ferric dicitrate


Subunit of: ferric dicitrate transport system

Subunit composition of ferric dicitrate transport system = [(FecE)2(FecC)(FecD)(FecB)][(FecA)([TonB][ExbB][ExbD])]
         ferric dicitrate ABC transporter = (FecE)2(FecC)(FecD)(FecB) (summary available)
                 ferric dicitrate ABC transporter - ATP binding subunit = FecE (summary available)
                 ferric dicitrate ABC transporter - membrane subunit = FecC (summary available)
                 ferric dicitrate ABC transporter - membrane subunit = FecD (summary available)
                 ferric dicitrate ABC transporter - periplasmic binding protein = FecB (summary available)
         ferric citrate outer membrane transport complex = (FecA)([TonB][ExbB][ExbD]) (summary available)
                 ferric citrate outer membrane porin FecA = FecA (summary available)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)

Locations: outer membrane, inner membrane, periplasmic space


GO Terms:
Cellular Component:
GO:0005886 - plasma membrane []
GO:0009279 - cell outer membrane []
GO:0030288 - outer membrane-bounded periplasmic space []

Enzymatic reaction of: transport of ferric dicitrate (ferric dicitrate transport system)

Inferred from experimentInferred by computational analysis

Transport reaction diagram for transport of ferric dicitrate


Subunit of ferric dicitrate ABC transporter: ferric dicitrate ABC transporter - ATP binding subunit

Gene: fecE Accession Numbers: EG10290 (EcoCyc), b4287, ECK4277

Locations: inner membrane

Sequence Length: 255 AAs

Molecular Weight: 28.191 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006811 - ion transport [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Inferred by computational analysisGO:0055072 - iron ion homeostasis [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, Staudenmaier89]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]

MultiFun Terms: cell processesadaptationsFe aquisition
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b4287, ModBase:P15031, PR:PRO_000022595, Protein Model Portal:P15031, RefSeq:NP_418707, SMR:P15031, UniProt:P15031

Relationship Links: EcoO157Cyc:Homolog:Z1964-MONOMER, EcoO157Cyc:Homolog:Z4385-MONOMER, EcoO157Cyc:Homolog:Z4919-MONOMER, InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF00005, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

Summary:
ATP binding component of the iron dicitrate ABC transporter

Essentiality data for fecE knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of ferric dicitrate ABC transporter: ferric dicitrate ABC transporter - membrane subunit

Gene: fecC Accession Numbers: EG10288 (EcoCyc), b4289, ECK4279

Locations: inner membrane

Sequence Length: 332 AAs

Molecular Weight: 34.893 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006811 - ion transport [UniProtGOA11a]
Inferred by computational analysisGO:0055072 - iron ion homeostasis [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0015623 - iron-chelate-transporting ATPase activity []
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell processesadaptationsFe aquisition
cell structuremembrane
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Unification Links: EcoliWiki:b4289, ModBase:P15030, PR:PRO_000022593, Pride:P15030, Protein Model Portal:P15030, RefSeq:NP_418709, SMR:P15030, UniProt:P15030

Relationship Links: EcoO157Cyc:Homolog:CHUU-MONOMER, EcoO157Cyc:Homolog:Z1965-MONOMER, EcoO157Cyc:Homolog:Z4383-MONOMER, EcoO157Cyc:Homolog:Z4384-MONOMER, InterPro:IN-FAMILY:IPR000522, InterPro:IN-FAMILY:IPR029022, Panther:IN-FAMILY:PTHR30472, Pfam:IN-FAMILY:PF01032

Summary:
FecC is one of two (along with FecD) integral membrane protein components of the iron dicitrate ABC transporter.

Essentiality data for fecC knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of ferric dicitrate ABC transporter: ferric dicitrate ABC transporter - membrane subunit

Gene: fecD Accession Numbers: EG10289 (EcoCyc), b4288, ECK4278

Locations: inner membrane

Sequence Length: 318 AAs

Molecular Weight: 34.131 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006811 - ion transport [UniProtGOA11a]
Inferred by computational analysisGO:0055072 - iron ion homeostasis [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0015623 - iron-chelate-transporting ATPase activity []
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell structuremembrane
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Unification Links: EcoliWiki:b4288, ModBase:P15029, PR:PRO_000022594, Pride:P15029, Protein Model Portal:P15029, RefSeq:NP_418708, SMR:P15029, UniProt:P15029

Relationship Links: EcoO157Cyc:Homolog:CHUU-MONOMER, EcoO157Cyc:Homolog:Z1965-MONOMER, EcoO157Cyc:Homolog:Z4384-MONOMER, InterPro:IN-FAMILY:IPR000522, InterPro:IN-FAMILY:IPR029022, Panther:IN-FAMILY:PTHR30472, Pfam:IN-FAMILY:PF01032

Summary:
FecD is one of two (along with FecC) integral membrane protein components of the iron dicitrate ABC transporter.

Essentiality data for fecD knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of ferric dicitrate ABC transporter: ferric dicitrate ABC transporter - periplasmic binding protein

Gene: fecB Accession Numbers: EG10287 (EcoCyc), b4290, ECK4280

Locations: periplasmic space

Sequence Length: 300 AAs

Molecular Weight: 33.146 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006811 - ion transport [UniProtGOA11a]
Inferred by computational analysisGO:0055072 - iron ion homeostasis [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0015623 - iron-chelate-transporting ATPase activity []
Cellular Component:
Inferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [DiazMejia09]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: cell processesadaptationsFe aquisition
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Unification Links: DIP:DIP-9584N, EcoliWiki:b4290, Mint:MINT-1275090, ModBase:P15028, PR:PRO_000022592, Protein Model Portal:P15028, RefSeq:NP_418710, SMR:P15028, UniProt:P15028

Relationship Links: InterPro:IN-FAMILY:IPR002491, Pfam:IN-FAMILY:PF01497, Prosite:IN-FAMILY:PS50983

Summary:
Periplasmic substrate binding component of the iron dicitrate ABC transporter

Essentiality data for fecB knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Harle95: Harle C, Kim I, Angerer A, Braun V (1995). "Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface." EMBO J 14(7);1430-8. PMID: 7729419

Hussein81: Hussein S, Hantke K, Braun V (1981). "Citrate-dependent iron transport system in Escherichia coli K-12." Eur J Biochem 1981;117(2);431-7. PMID: 6268411

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pressler88: Pressler U, Staudenmaier H, Zimmermann L, Braun V (1988). "Genetics of the iron dicitrate transport system of Escherichia coli." J Bacteriol 170(6);2716-24. PMID: 2836368

Staudenmaier89: Staudenmaier H, Van Hove B, Yaraghi Z, Braun V (1989). "Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli." J Bacteriol 171(5);2626-33. PMID: 2651410

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wagegg81: Wagegg W, Braun V (1981). "Ferric citrate transport in Escherichia coli requires outer membrane receptor protein fecA." J Bacteriol 145(1);156-63. PMID: 7007312

Zimmermann84: Zimmermann L, Hantke K, Braun V (1984). "Exogenous induction of the iron dicitrate transport system of Escherichia coli K-12." J Bacteriol 159(1);271-7. PMID: 6376472


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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