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Escherichia coli K-12 substr. MG1655 Polypeptide: putative transport protein



Gene: araJ Accession Numbers: EG10060 (EcoCyc), b0396, ECK0390

Synonyms: araT, AraJ MFS transporter

Regulation Summary Diagram: ?

Summary:
The AraJ protein is an uncharacterised member of the major facilitator superfamily (MFS) of transporters [Pao98]. Expression of araJ is under the control of an arabinose-inducible promoter controlled by the AraC transcriptional regulator [Kosiba82, Hendrickson90]. araJ encodes a largely hydrophobic protein with a possible signal sequence [Reeder91]. AraJ shares sequence similarity with drug efflux proteins of the MFS [Saier14].

Disruption of araJ does not have an apparent effect on arabinose uptake or utilisation [Reeder91]. AraJ is not essential for the intracellular arabinose depletion that is associated with 'off-switching' of the inducible arabinose utilization system [Fritz14]

Locations: inner membrane

Map Position: [410,521 <- 411,705] (8.85 centisomes)
Length: 1185 bp / 394 aa

Molecular Weight of Polypeptide: 41.926 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001378 , CGSC:29861 , EchoBASE:EB0058 , EcoGene:EG10060 , EcoliWiki:b0396 , ModBase:P23910 , OU-Microarray:b0396 , PortEco:araJ , PR:PRO_000022122 , Protein Model Portal:P23910 , RefSeq:NP_414930 , RegulonDB:EG10060 , SMR:P23910 , String:511145.b0396 , UniProt:P23910

Relationship Links: InterPro:IN-FAMILY:IPR000576 , InterPro:IN-FAMILY:IPR011701 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR020846 , Panther:IN-FAMILY:PTHR24003:SF271 , Pfam:IN-FAMILY:PF07690 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 26 (5 members) , 120 (2 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for araJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 5 -> 27
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 42 -> 63
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 70 -> 89
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 94 -> 116
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 129 -> 151
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 156 -> 178
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 199 -> 221
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 236 -> 255
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 262 -> 284
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 294 -> 316
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 323 -> 342
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 352 -> 374
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0396 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10060; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fritz14: Fritz G, Megerle JA, Westermayer SA, Brick D, Heermann R, Jung K, Radler JO, Gerland U (2014). "Single Cell Kinetics of Phenotypic Switching in the Arabinose Utilization System of E. coli." PLoS One 9(2);e89532. PMID: 24586851

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hendrickson90: Hendrickson W, Stoner C, Schleif R (1990). "Characterization of the Escherichia coli araFGH and araJ promoters." J Mol Biol 1990;215(4);497-510. PMID: 2231717

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kosiba82: Kosiba BE, Schleif R (1982). "Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence." J Mol Biol 1982;156(1);53-66. PMID: 7047752

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Reeder91: Reeder T, Schleif R (1991). "Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon." J Bacteriol 1991;173(24);7765-71. PMID: 1744033

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Gallegos97: Gallegos MT, Schleif R, Bairoch A, Hofmann K, Ramos JL (1997). "Arac/XylS family of transcriptional regulators." Microbiol Mol Biol Rev 61(4);393-410. PMID: 9409145


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.