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Escherichia coli K-12 substr. MG1655 Polypeptide: L-arginine ABC transporter - ATP binding subunit



Gene: artP Accession Numbers: EG11624 (EcoCyc), b0864, ECK0855

Regulation Summary Diagram: ?

Component of:
predicted ABC transporter ArtPQMI (summary available)
L-arginine ABC transporter (summary available)

Summary:
ArtP is predicted to be the ATP-binding subunit of an L- arginine ABC transporter. Sequence analysis indicates that ArtP is highly homologous to the HisP ATP-binding protein of the histine/LAO (lysine/arginine/ornithine) ABC transporter system in Salmonella typhimurium. artP contains a conserved ATP-binding consensus site [Wissenbach95].

Gene Citations: [Wissenbach93]

Locations: inner membrane

Map Position: [902,229 <- 902,957] (19.45 centisomes)
Length: 729 bp / 242 aa

Molecular Weight of Polypeptide: 27.022 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002939 , CGSC:31677 , DIP:DIP-47917N , EchoBASE:EB1581 , EcoGene:EG11624 , EcoliWiki:b0864 , Mint:MINT-1236053 , ModBase:P0AAF6 , OU-Microarray:b0864 , PortEco:artP , PR:PRO_000022159 , Pride:P0AAF6 , Protein Model Portal:P0AAF6 , RefSeq:NP_415385 , RegulonDB:EG11624 , SMR:P0AAF6 , String:511145.b0864 , Swiss-Model:P0AAF6 , UniProt:P0AAF6

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:1902765 - L-arginine import into cell Inferred from experiment [Wissenbach95]
GO:0003333 - amino acid transmembrane transport Inferred by computational analysis [Wissenbach95]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015426 - polar-amino acid-transporting ATPase activity Inferred by computational analysis [Wissenbach95]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, LopezCampistrou05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Inferred by computational analysis [Wissenbach95]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids arginine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for artP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: predicted ABC transporter ArtPQMI

Subunit composition of predicted ABC transporter ArtPQMI = [ArtI][ArtM][ArtQ][ArtP]2
         periplasmic binding protein ArtI = ArtI (summary available)
         L-arginine ABC transporter - membrane subunit = ArtM (summary available)
         L-arginine ABC transporter - membrane subunit = ArtQ (summary available)
         L-arginine ABC transporter - ATP binding subunit = ArtP (summary available)

Summary:
The art gene cluster contains two transcription units: artPIQM and artJ [Wissenbach95] which are regulated by the the arginine repressor, ArgR [Caldara06, Caldara07]. Sequence analysis indicates that ArtQ, ArtP and ArtM are the membrane components of an ATP dependent transporter, and ArtJ has been experimentally characterised as an L-arginine binding periplasmic protein. ArtI exhibits homology with a number of ABC transporter periplasmic binding proteins, but its substrate is not known. Purified ArtI does not bind any of the common or uncommon amino acids nor putrescine [Linton98, Wissenbach95]. It is assumed that ArtJ and ArtI are periplasmic binding proteins that share the ArtQPM membrane proteins [Wissenbach95].

Credits:
Created 07-May-2014 by Mackie A , Macquarie University
Last-Curated ? 08-May-2014 by Mackie A , Macquarie University


Subunit of: L-arginine ABC transporter

Subunit composition of L-arginine ABC transporter = [ArtJ][ArtQ][ArtM][ArtP]2
         L-arginine ABC transporter - periplasmic binding protein = ArtJ (summary available)
         L-arginine ABC transporter - membrane subunit = ArtQ (summary available)
         L-arginine ABC transporter - membrane subunit = ArtM (summary available)
         L-arginine ABC transporter - ATP binding subunit = ArtP (summary available)

Summary:
ArtPQMJ is an L-arginine transporter that is a member of the ATP Binding Cassette (ABC) superfamily [Linton98, Moussatova08]. Sequence analysis indicates that ArtQ and ArtM are the integral membrane components and ArtP forms the ATP binding domain. ArtJ is a periplasmic binding protein with high affinity for L-arginine [Wissenbach95].

The art gene cluster contains two transcription units: artPIQM and artJ [Wissenbach95] which are regulated by the the arginine repressor, ArgR [Caldara06, Caldara07]. ArtI exhibits homology with a number of ABC transporter periplasmic binding proteins, but its substrate is not known [Linton98]. Purified ArtI does not bind any of the common or uncommon amino acids or putrescine [Wissenbach95].

Credits:
Revised 07-May-2014 by Mackie A , Macquarie University


Enzymatic reaction of: L-arginine transporter (L-arginine ABC transporter)


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 3 -> 241
[UniProt09]
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 35 -> 42
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Sequence-Conflict 94 -> 95
[Wissenbach93, UniProt10]
Alternate sequence: WP → CA; UniProt: (in Ref. 1; CAA60101);
Sequence-Conflict 121
[Wissenbach93, UniProt10]
Alternate sequence: R → S; UniProt: (in Ref. 1; CAA60101);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0864 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11624; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Caldara06: Caldara M, Charlier D, Cunin R (2006). "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation." Microbiology 152(Pt 11);3343-54. PMID: 17074904

Caldara07: Caldara M, Minh PN, Bostoen S, Massant J, Charlier D (2007). "ArgR-dependent Repression of Arginine and Histidine Transport Genes in Escherichia coli K-12." J Mol Biol 373(2);251-67. PMID: 17850814

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Linton98: Linton KJ, Higgins CF (1998). "The Escherichia coli ATP-binding cassette (ABC) proteins." Mol Microbiol 1998;28(1);5-13. PMID: 9593292

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Moussatova08: Moussatova A, Kandt C, O'Mara ML, Tieleman DP (2008). "ATP-binding cassette transporters in Escherichia coli." Biochim Biophys Acta 1778(9);1757-71. PMID: 18634750

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wissenbach93: Wissenbach U, Keck B, Unden G (1993). "Physical map location of the new artPIQMJ genes of Escherichia coli, encoding a periplasmic arginine transport system." J Bacteriol 175(11);3687-8. PMID: 8501075

Wissenbach95: Wissenbach U, Six S, Bongaerts J, Ternes D, Steinwachs S, Unden G (1995). "A third periplasmic transport system for L-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport." Mol Microbiol 1995;17(4);675-86. PMID: 8801422

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Lacour04: Lacour S, Landini P (2004). "SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences." J Bacteriol 186(21);7186-95. PMID: 15489429

Makarova01: Makarova KS, Mironov AA, Gelfand MS (2001). "Conservation of the binding site for the arginine repressor in all bacterial lineages." Genome Biol 2(4);RESEARCH0013. PMID: 11305941


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13A.