Escherichia coli K-12 substr. MG1655 Polypeptide: AtoC transcriptional activator; Az protein inhibitor of ODC

Gene: atoC Accession Numbers: EG11668 (EcoCyc), b2220, ECK2213

Synonyms: AtoC response regulator

Regulation Summary Diagram: ?

Regulation summary diagram for atoC

Alternative forms of AtoC transcriptional activator; Az protein inhibitor of ODC: AtoC-phosphorylated DNA-binding transcriptional activator (extended summary available)

Citations: [Canellakis93, Jenkins87a, Pao95, Parkinson92, Stock90, Parkinson93]

Locations: cytosol

Map Position: [2,319,888 -> 2,321,273] (50.0 centisomes, 180°)
Length: 1386 bp / 461 aa

Molecular Weight of Polypeptide: 52.176 kD (from nucleotide sequence)

pI: 6.24

Unification Links: ASAP:ABE-0007343 , CGSC:984 , DIP:DIP-9190N , EchoBASE:EB1619 , EcoGene:EG11668 , EcoliWiki:b2220 , ModBase:Q06065 , OU-Microarray:b2220 , PortEco:atoC , PR:PRO_000022182 , Pride:Q06065 , Protein Model Portal:Q06065 , RegulonDB:EG11668 , SMR:Q06065 , String:511145.b2220 , Swiss-Model:Q06065 , UniProt:Q06065

Relationship Links: InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR002078 , InterPro:IN-FAMILY:IPR002197 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR009057 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR025662 , InterPro:IN-FAMILY:IPR025943 , InterPro:IN-FAMILY:IPR025944 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00158 , Pfam:IN-FAMILY:PF02954 , Prints:IN-FAMILY:PR01590 , Prosite:IN-FAMILY:PS00675 , Prosite:IN-FAMILY:PS00676 , Prosite:IN-FAMILY:PS00688 , Prosite:IN-FAMILY:PS50045 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00382 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 105 (10 members) , 121 (40 members)

Reactions known to consume the compound:

AtoSC Two-Component Signal Transduction System :
AtoS-P + AtoC → AtoS monomer + AtoC-P

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0043086 - negative regulation of catalytic activity Inferred from experiment [Canellakis93]
GO:0045893 - positive regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [Canellakis93, Jenkins87a]
GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [UniProtGOA11a, GOA01a, Gaudet10]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [Canellakis93, Jenkins87a]
GO:0000156 - phosphorelay response regulator activity Inferred by computational analysis [Gaudet10]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008134 - transcription factor binding Inferred by computational analysis [GOA01a]
GO:0043565 - sequence-specific DNA binding Inferred by computational analysis [GOA01a, Gaudet10]
GO:0044212 - transcription regulatory region DNA binding Inferred by computational analysis [Gaudet10]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism carbon utilization fatty acids
metabolism central intermediary metabolism polyamine biosynthesis
regulation genetic unit regulated operon
regulation type of regulation posttranscriptional inhibition / activation of enzymes
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Transcription Units regulated by related protein AtoC-phosphorylated DNA-binding transcriptional activator (1 total):

Transcription-unit diagram

Essentiality data for atoC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of AtoC transcriptional activator; Az protein inhibitor of ODC with features indicated

Feature Class Location Citations Comment State
Conserved-Region 6 -> 120
UniProt: Response regulatory;
4-aspartylphosphate-Modification 55
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Sequence-Conflict 99
[Canellakis93, UniProt10]
UniProt: (in Ref. 5; AAA23450);
Conserved-Region 145 -> 374
UniProt: Sigma-54 factor interaction;
Nucleotide-Phosphate-Binding-Region 236 -> 245
UniProt: ATP; Non-Experimental Qualifier: potential;
DNA-Binding-Region 433 -> 452
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2220 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11668; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Canellakis93: Canellakis ES, Paterakis AA, Huang SC, Panagiotidis CA, Kyriakidis DA (1993). "Identification, cloning, and nucleotide sequencing of the ornithine decarboxylase antizyme gene of Escherichia coli." Proc Natl Acad Sci U S A 1993;90(15);7129-33. PMID: 8346225

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jenkins87a: Jenkins LS, Nunn WD (1987). "Regulation of the ato operon by the atoC gene in Escherichia coli." J Bacteriol 1987;169(5);2096-102. PMID: 2883171

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Filippou07: Filippou PS, Lioliou EE, Panagiotidis CA, Athanassopoulos CM, Garnelis T, Papaioannou D, Kyriakidis DA (2007). "Effect of polyamines and synthetic polyamine-analogues on the expression of antizyme (AtoC) and its regulatory genes." BMC Biochem 8;1. PMID: 17224065

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, biocyc13.