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Escherichia coli K-12 substr. MG1655 Polypeptide: YagG GPH Transporter



Gene: yagG Accession Numbers: G6142 (EcoCyc), b0270, ECK0271

Regulation Summary Diagram: ?

Summary:
The YagG protein is an uncharacterized member of the GPH family of galactose-pentose-hexuronide transporters [Poolman96]. Based on sequence similarity, it may function as a proton or other ion-driven sugar uptake system.

Locations: inner membrane

Map Position: [284,619 -> 286,001] (6.13 centisomes)
Length: 1383 bp / 460 aa

Molecular Weight of Polypeptide: 50.632 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000929 , DIP:DIP-11234N , EchoBASE:EB3130 , EcoGene:EG13346 , EcoliWiki:b0270 , Mint:MINT-1263876 , ModBase:P75683 , OU-Microarray:b0270 , PortEco:yagG , Protein Model Portal:P75683 , RefSeq:NP_414804 , RegulonDB:G6142 , String:511145.b0270 , UniProt:P75683

Relationship Links: EcoO157Cyc:Homolog:Z5001 , EcoO157Cyc:Homolog:Z5001-MONOMER , InterPro:IN-FAMILY:IPR001927 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR018043 , Pfam:IN-FAMILY:PF07690 , Prosite:IN-FAMILY:PS00872

In Paralogous Gene Group: 83 (6 members)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006814 - sodium ion transport Inferred by computational analysis [GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for yagG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 10 -> 30
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 31 -> 51
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 79 -> 99
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 107 -> 127
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 151 -> 171
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 180 -> 200
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 263 -> 283
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 309 -> 329
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 367 -> 387
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 370
[Chung97, UniProt10]
Alternate sequence: K → N; UniProt: (in Ref. 1; AAB08691);
Transmembrane-Region 406 -> 426
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chung97: Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. (1997). "Sequence of minutes 4-25 of Escherichia coli." Data submission to EMBL/GenBank/DDBJ databases on 1997-01.

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Poolman96: Poolman B, Knol J, van der Does C, Henderson PJ, Liang WJ, Leblanc G, Pourcher T, Mus-Veteau I (1996). "Cation and sugar selectivity determinants in a novel family of transport proteins." Mol Microbiol 1996;19(5);911-22. PMID: 8830272

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc13.