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Escherichia coli K-12 substr. MG1655 Polypeptide: YgcS MFS transporter



Gene: ygcS Accession Numbers: G7437 (EcoCyc), b2771, ECK2766

Regulation Summary Diagram: ?

Summary:
The YgcS protein is an uncharacterised member of the major facilitator superfamily (MFS) of transporters [Pao98]. Based on sequence similarity, YgcS may function as a proton-driven metabolite uptake system.

Locations: inner membrane

Map Position: [2,894,555 <- 2,895,892] (62.39 centisomes)
Length: 1338 bp / 445 aa

Molecular Weight of Polypeptide: 48.234 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009082 , EchoBASE:EB2927 , EcoGene:EG13126 , EcoliWiki:b2771 , ModBase:Q46909 , OU-Microarray:b2771 , PortEco:ygcS , Pride:Q46909 , Protein Model Portal:Q46909 , RefSeq:NP_417251 , RegulonDB:G7437 , SMR:Q46909 , String:511145.b2771 , UniProt:Q46909

Relationship Links: InterPro:IN-FAMILY:IPR005828 , InterPro:IN-FAMILY:IPR005829 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR020846 , Pfam:IN-FAMILY:PF00083 , Prosite:IN-FAMILY:PS00216 , Prosite:IN-FAMILY:PS00217 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 127 (2 members) , 136 (7 members)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0022857 - transmembrane transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for ygcS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 23 -> 43
[UniProt10a]
UniProt: Helical; Name=1;; Non-Experimental Qualifier: potential;
Transmembrane-Region 57 -> 77
[UniProt10a]
UniProt: Helical; Name=2;; Non-Experimental Qualifier: potential;
Transmembrane-Region 86 -> 106
[UniProt10a]
UniProt: Helical; Name=3;; Non-Experimental Qualifier: potential;
Transmembrane-Region 115 -> 135
[UniProt10a]
UniProt: Helical; Name=4;; Non-Experimental Qualifier: potential;
Transmembrane-Region 143 -> 163
[UniProt10a]
UniProt: Helical; Name=5;; Non-Experimental Qualifier: potential;
Transmembrane-Region 176 -> 196
[UniProt10a]
UniProt: Helical; Name=6;; Non-Experimental Qualifier: potential;
Transmembrane-Region 254 -> 274
[UniProt10a]
UniProt: Helical; Name=7;; Non-Experimental Qualifier: potential;
Transmembrane-Region 287 -> 307
[UniProt10a]
UniProt: Helical; Name=8;; Non-Experimental Qualifier: potential;
Transmembrane-Region 312 -> 332
[UniProt10a]
UniProt: Helical; Name=9;; Non-Experimental Qualifier: potential;
Transmembrane-Region 338 -> 358
[UniProt10a]
UniProt: Helical; Name=10;; Non-Experimental Qualifier: potential;
Transmembrane-Region 370 -> 390
[UniProt10a]
UniProt: Helical; Name=11;; Non-Experimental Qualifier: potential;
Transmembrane-Region 401 -> 421
[UniProt10a]
UniProt: Helical; Name=12;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.