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Escherichia coli K-12 substr. MG1655 Polypeptide: BasR transcriptional regulator



Gene: basR Accession Numbers: EG11615 (EcoCyc), b4113, ECK4106

Synonyms: pmrA, BasR response regulator

Regulation Summary Diagram: ?

Alternative forms of BasR transcriptional regulator: BasR-Phosphorylated transcriptional regulator (extended summary available)

Summary:
The transcriptional regulatory protein BasR is part of the two-component BasS/BasR signal transduction system [Nagasawa93]. BasS functions as a membrane-associated protein kinase that phosphorylates BasR in response to elevated levels of Fe(III) which can permeabilize the outer membrane and result in cell death [Yamamoto05, Nagasawa93, Chamnongpol02]. Phosphorylation of BasR increases the affinity for its specific DNA binding sites, leading to the transcriptional expression of several genes involved in modification of lipopolysaccharide to prevent excessiveFe(III) binding [Hagiwara04].

Deletion of basR resulted in susceptibility to cell-killing by elevated levels of Fe(III) [Chamnongpol02]. Deletion of basSR resulted in acid sensitivity during growth at elevated iron concentrations [Hagiwara04]. Expression of the arnBCADTEF operon increased during growth with elevated FeSO4 or FeCl3 and was dependent upon the BasSR two-component signal transduction system [Hagiwara04]. Deletion of basR prevents the FeSO4-, ZnSO4-, and NH4VO3-mediated induction of eptA, arnB, and yibD and results in sensitivity to the cationic agent polymyxin B [Froelich06]. A basRG53V (constitutive) mutant is resistant to polymyxin B and colistin, sensitive to the anionic agent deoxycholic acid, and expresses eptA, arnB, and yibD at high levels [Froelich06]. Activation of the BasSR two-component system by Fe3+ results in the post-translational inhibition of LpxT and a near total loss of 1-diphosphate lipid A from the cell surface [Herrera10]. Zn2+ inhibits growth of a basR mutant slightly [Lee05d]. The antimicrobial peptide lactoferricin B, binds to BasR and inhibits its phosphorylation in vitro [Ho11].

Expression of basR increased during growth with ZnSO4 [Lee05d].

Citations: [Pao95, Parkinson92, Stock90, Parkinson93, Zhou99a, Nummila95, Groisman01, Winfield04, Gibbons05, Fu07]

Locations: cytosol

Map Position: [4,331,305 <- 4,331,973] (93.35 centisomes)
Length: 669 bp / 222 aa

Molecular Weight of Polypeptide: 25.031 kD (from nucleotide sequence)

pI: 5.99

Unification Links: ASAP:ABE-0013466 , CGSC:28168 , DIP:DIP-9201N , EchoBASE:EB1572 , EcoGene:EG11615 , EcoliWiki:b4113 , ModBase:P30843 , OU-Microarray:b4113 , PortEco:basR , PR:PRO_000022199 , Pride:P30843 , Protein Model Portal:P30843 , RegulonDB:EG11615 , SMR:P30843 , String:511145.b4113 , UniProt:P30843

Relationship Links: InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR001867 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00486 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00448 , Smart:IN-FAMILY:SM00862

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

BasSR Two-Component Signal Transduction System :
BasS sensory histidine kinase - phosphorylated[inner membrane] + BasR → BasS[inner membrane] + BasR-Pasp51

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Yamamoto05]
GO:0006355 - regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Froelich06]
GO:0010041 - response to iron(III) ion Inferred from experiment [Hagiwara04, Chamnongpol02]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0046677 - response to antibiotic Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0000156 - phosphorelay response regulator activity Inferred from experiment [Yamamoto05]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

DNA binding site length: 20 base-pairs

Transcription Units regulated by related protein BasR-Phosphorylated transcriptional regulator (11 total):

Essentiality data for basR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 01-Aug-2008 by Johnson A , JCVI


Sequence Features

Feature Class Location Citations Comment State
Conserved-Region 1 -> 116
[UniProt09]
UniProt: Response regulatory;
 
Sequence-Conflict 29
[Nagasawa93, UniProt10a]
Alternate sequence: S → T; UniProt: (in Ref. 1);
 
Extrinsic-Sequence-Variant 29
[UniProt10a]
Alternate sequence: S → G; UniProt: (in strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8 and ECOR 9);
 
Extrinsic-Sequence-Variant 31
[UniProt10a]
Alternate sequence: T → S; UniProt: (in strain: ECOR 35, ECOR 36, ECOR 38, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64, ECOR 65 and ECOR 66);
 
4-aspartylphosphate-Modification 51
[UniProt11a]
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Unmodified
Extrinsic-Sequence-Variant 128
[UniProt10a]
Alternate sequence: I → N; UniProt: (in strain: ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64);
 
Extrinsic-Sequence-Variant 129
[UniProt10a]
Alternate sequence: V → L; UniProt: (in strain: ECOR 19);
 
Extrinsic-Sequence-Variant 144
[UniProt10a]
Alternate sequence: G → S; UniProt: (in strain: ECOR 44, ECOR 48, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and ECOR 72);
 
Extrinsic-Sequence-Variant 207
[UniProt10a]
Alternate sequence: R → P; UniProt: (in strain: ECOR 27);
 
Extrinsic-Sequence-Variant 214
[UniProt10a]
Alternate sequence: Y → F; UniProt: (in strain: ECOR 27);
 


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b4113 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11615; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chamnongpol02: Chamnongpol S, Dodson W, Cromie MJ, Harris ZL, Groisman EA (2002). "Fe(III)-mediated cellular toxicity." Mol Microbiol 45(3);711-9. PMID: 12139617

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Froelich06: Froelich JM, Tran K, Wall D (2006). "A pmrA constitutive mutant sensitizes Escherichia coli to deoxycholic acid." J Bacteriol 188(3);1180-3. PMID: 16428424

Fu07: Fu W, Yang F, Kang X, Zhang X, Li Y, Xia B, Jin C (2007). "First structure of the polymyxin resistance proteins." Biochem Biophys Res Commun 361(4);1033-7. PMID: 17686460

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Gibbons05: Gibbons HS, Kalb SR, Cotter RJ, Raetz CR (2005). "Role of Mg2+ and pH in the modification of Salmonella lipid A after endocytosis by macrophage tumour cells." Mol Microbiol 55(2);425-40. PMID: 15659161

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Groisman01: Groisman EA (2001). "The pleiotropic two-component regulatory system PhoP-PhoQ." J Bacteriol 183(6);1835-42. PMID: 11222580

Hagiwara04: Hagiwara D, Yamashino T, Mizuno T (2004). "A Genome-wide view of the Escherichia coli BasS-BasR two-component system implicated in iron-responses." Biosci Biotechnol Biochem 68(8);1758-67. PMID: 15322361

Herrera10: Herrera CM, Hankins JV, Trent MS (2010). "Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides." Mol Microbiol 76(6);1444-60. PMID: 20384697

Ho11: Ho YH, Sung TC, Chen CS (2011). "Lactoferricin B inhibits the phosphorylation of the two-component system response regulators BasR and CreB." Mol Cell Proteomics. PMID: 22138548

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lee05d: Lee LJ, Barrett JA, Poole RK (2005). "Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc." J Bacteriol 187(3);1124-34. PMID: 15659689

Nagasawa93: Nagasawa S, Ishige K, Mizuno T (1993). "Novel members of the two-component signal transduction genes in Escherichia coli." J Biochem (Tokyo) 1993;114(3);350-7. PMID: 8282725

Nummila95: Nummila K, Kilpelainen I, Zahringer U, Vaara M, Helander IM (1995). "Lipopolysaccharides of polymyxin B-resistant mutants of Escherichia coli are extensively substituted by 2-aminoethyl pyrophosphate and contain aminoarabinose in lipid A." Mol Microbiol 16(2);271-8. PMID: 7565089

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

PerezRueda04: Perez-Rueda E, Collado-Vides J, Segovia L (2004). "Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea." Comput Biol Chem 28(5-6);341-50. PMID: 15556475

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Winfield04: Winfield MD, Groisman EA (2004). "Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes." Proc Natl Acad Sci U S A 101(49);17162-7. PMID: 15569938

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Zhou99a: Zhou Z, Lin S, Cotter RJ, Raetz CR (1999). "Lipid A modifications characteristic of Salmonella typhimurium are induced by NH4VO3 in Escherichia coli K12. Detection of 4-amino-4-deoxy-L-arabinose, phosphoethanolamine and palmitate." J Biol Chem 274(26);18503-14. PMID: 10373459


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.