Escherichia coli K-12 substr. MG1655 Polypeptide: CheB

Gene: cheB Accession Numbers: EG10147 (EcoCyc), b1883, ECK1884

Synonyms: protein-glutamate methylesterase, unmodified regulatory protein cheB, chemotaxis protein cheB

Regulation Summary Diagram: ?

Regulation summary diagram for cheB

Alternative forms of CheB: CheB-Pasp

The CheB protein consists of two distinct domains: a regulatory domain that accepts a phosphoryl group and a catalytic domain that catalyzes the demethylation and deamidation of MCPs. [Hoch95, Neidhardt96]

Gene Citations: [Kundu97, Arnosti89, Slocum83, Parkinson82, Parkinson78]

Locations: inner membrane, cytosol

Map Position: [1,965,476 <- 1,966,525] (42.36 centisomes, 153°)
Length: 1050 bp / 349 aa

Molecular Weight of Polypeptide: 37.468 kD (from nucleotide sequence)

pI: 8.85

Unification Links: ASAP:ABE-0006284 , CGSC:927 , DIP:DIP-9272N , EchoBASE:EB0145 , EcoGene:EG10147 , EcoliWiki:b1883 , ModBase:P07330 , OU-Microarray:b1883 , PortEco:cheB , PR:PRO_000022278 , Protein Model Portal:P07330 , RegulonDB:EG10147 , SMR:P07330 , String:511145.b1883 , Swiss-Model:P07330 , UniProt:P07330

Relationship Links: InterPro:IN-FAMILY:IPR000673 , InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR008248 , InterPro:IN-FAMILY:IPR011006 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF01339 , Prosite:IN-FAMILY:PS50110 , Prosite:IN-FAMILY:PS50122 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

Chemotactic Two-Component Signal Transduction :
CheA-P + CheB → CheA + CheB-Pasp

In Reactions of unknown directionality:

Not in pathways:
CheB-Pasp + H2O = CheB + phosphate

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for cheB

GO Terms:

Biological Process: GO:0006935 - chemotaxis Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a, Armstrong69]
GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [GOA06, GOA01a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01, GOA01a]
Molecular Function: GO:0000156 - phosphorelay response regulator activity Inferred by computational analysis [GOA01a]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA06]
GO:0008984 - protein-glutamate methylesterase activity Inferred by computational analysis [GOA06, GOA01, GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ridgway77]
GO:0005886 - plasma membrane Inferred from experiment [Ridgway77]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, GOA01a]

MultiFun Terms: cell processes motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
information transfer protein related posttranslational modification

Essentiality data for cheB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Protein sequence of CheB with features indicated

Feature Class Location Citations Comment
Conserved-Region 5 -> 122
UniProt: Response regulatory;
4-aspartylphosphate-Modification 56
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Phosphorylation-Modification 56
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 96
[Mutoh86, UniProt10a]
UniProt: (in Ref. 1; AAA23569);
Sequence-Conflict 125
[Mutoh86, UniProt10a]
UniProt: (in Ref. 1; AAA23569);
Conserved-Region 152 -> 344
UniProt: CheB-type methylesterase;
Active-Site 164
UniProt: Non-Experimental Qualifier: by similarity;
Active-Site 190
UniProt: Non-Experimental Qualifier: by similarity;
Active-Site 286
UniProt: Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b1883 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10147; confirmed by SwissProt match.


Armstrong69: Armstrong JB, Adler J (1969). "Complementation of nonchemotactic mutants of Escherichia coli." Genetics 61(1);61-6. PMID: 4895809

Arnosti89: Arnosti DN, Chamberlin MJ (1989). "Secondary sigma factor controls transcription of flagellar and chemotaxis genes in Escherichia coli." Proc Natl Acad Sci U S A 86(3);830-4. PMID: 2644646

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Hoch95: Hoch, JA, Silhavy, TJ "Two-Component Signal Transduction." ASM Press, Washington, D.C. 1995.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kundu97: Kundu TK, Kusano S, Ishihama A (1997). "Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes." J Bacteriol 179(13);4264-9. PMID: 9209042

Mutoh86: Mutoh N, Simon MI (1986). "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli." J Bacteriol 165(1);161-6. PMID: 3510184

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Parkinson78: Parkinson JS (1978). "Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis." J Bacteriol 135(1);45-53. PMID: 353036

Parkinson82: Parkinson JS, Houts SE (1982). "Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions." J Bacteriol 151(1);106-13. PMID: 7045071

Ridgway77: Ridgway HG, Silverman M, Simon MI (1977). "Localization of proteins controlling motility and chemotaxis in Escherichia coli." J Bacteriol 132(2);657-65. PMID: 334749

Slocum83: Slocum MK, Parkinson JS (1983). "Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: organization of the tar region." J Bacteriol 155(2);565-77. PMID: 6307970

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Constantinidou06: Constantinidou C, Hobman JL, Griffiths L, Patel MD, Penn CW, Cole JA, Overton TW (2006). "A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth." J Biol Chem 281(8);4802-15. PMID: 16377617

Helmann87: Helmann JD, Chamberlin MJ (1987). "DNA sequence analysis suggests that expression of flagellar and chemotaxis genes in Escherichia coli and Salmonella typhimurium is controlled by an alternative sigma factor." Proc Natl Acad Sci U S A 84(18);6422-4. PMID: 3306678

Ide99: Ide N, Ikebe T, Kutsukake K (1999). "Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella." Genes Genet Syst 74(3);113-6. PMID: 10586520

Ko00b: Ko M, Park C (2000). "Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli." J Mol Biol 303(3);371-82. PMID: 11031114

Liu95: Liu X, Matsumura P (1995). "An alternative sigma factor controls transcription of flagellar class-III operons in Escherichia coli: gene sequence, overproduction, purification and characterization." Gene 164(1);81-4. PMID: 7590326

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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