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Escherichia coli K-12 substr. MG1655 Enzyme: citrate lyase synthetase



Gene: citC Accession Numbers: G6344 (EcoCyc), b0618, ECK0611

Synonyms: ybeO

Regulation Summary Diagram: ?

Summary:
Citrate lyase synthetase activates citrate lyase by acetylation [Nilekani83]. The actual site of acetylation is the prosthetic group of the acyl-carrier protein, or γ, subunit.

Locations: cytosol

Map Position: [650,021 <- 651,079] (14.01 centisomes)
Length: 1059 bp / 352 aa

Molecular Weight of Polypeptide: 40.077 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002126 , EchoBASE:EB3409 , EcoGene:EG13645 , EcoliWiki:b0618 , ModBase:P77390 , OU-Microarray:b0618 , PortEco:citC , PR:PRO_000022288 , Pride:P77390 , Protein Model Portal:P77390 , RefSeq:NP_415151 , RegulonDB:G6344 , SMR:P77390 , String:511145.b0618 , UniProt:P77390

Relationship Links: InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR004821 , InterPro:IN-FAMILY:IPR005216 , InterPro:IN-FAMILY:IPR013166 , InterPro:IN-FAMILY:IPR014729 , InterPro:IN-FAMILY:IPR016181 , Pfam:IN-FAMILY:PF08218 , Prosite:IN-FAMILY:PS51186 , Smart:IN-FAMILY:SM00764

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006464 - cellular protein modification process Inferred from experiment [Nilekani83]
GO:0009058 - biosynthetic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0008771 - [citrate (pro-3S)-lyase] ligase activity Inferred from experiment Inferred by computational analysis [GOA01a, GOA01, Nilekani83]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11]
GO:0008080 - N-acetyltransferase activity Inferred by computational analysis [GOA01]
GO:0016874 - ligase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation

Essentiality data for citC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 08-Feb-2006 by Keseler I , SRI International


Enzymatic reaction of: citrate lyase synthetase

Synonyms: [citrate(pro-3S)-lyase] ligase, citrate lyase ligase, acetate:SH-citrate lyase ligase

EC Number: 6.2.1.22

[a holo citrate lyase acyl-carrier protein] + acetate + ATP <=> an acetyl-[holo citrate lyase acyl-carrier protein] + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: citrate lyase activation


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 128
[UniProt09]
UniProt: N-acetyltransferase;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Nilekani83: Nilekani S, SivaRaman C (1983). "Purification and properties of citrate lyase from Escherichia coli." Biochemistry 1983;22(20);4657-63. PMID: 6354265

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bobon: Bobon J, Parent M, Toussaint C, Pinchard A "[Long-acting neuroleptics. IV. Preliminary study of clopimozide (R 29764)]." Acta Psychiatr Belg 76(1);138-43. PMID: 970182

Ingmer98: Ingmer H, Miller CA, Cohen SN (1998). "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator." Mol Microbiol 29(1);49-59. PMID: 9701802

Scheu12: Scheu PD, Witan J, Rauschmeier M, Graf S, Liao YF, Ebert-Jung A, Basche T, Erker W, Unden G (2012). "CitA/CitB two-component system regulating citrate fermentation in Escherichia coli and its relation to the DcuS/DcuR system in vivo." J Bacteriol 194(3);636-45. PMID: 22101843

Yamamoto08a: Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A (2008). "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli." Biosci Biotechnol Biochem 72(11);3011-4. PMID: 18997424


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc11.