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Escherichia coli K-12 substr. MG1655 Protein: citrate lyase, citrate-ACP transferase component



Gene: citF Accession Numbers: G6341 (EcoCyc), b0615, ECK0608

Synonyms: ybdV

Regulation Summary Diagram: ?

Component of: citrate lyase, inactive

Subunit composition of citrate lyase, citrate-ACP transferase component = [CitF]6
         citrate lyase, citrate-ACP transferase α subunit = CitF

Alternative forms of citrate lyase, inactive: citrate lyase (extended summary available)

Locations: cytosol

Map Position: [647,262 <- 648,794] (13.95 centisomes)
Length: 1533 bp / 510 aa

Molecular Weight of Polypeptide: 55.173 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002119 , EchoBASE:EB3311 , EcoGene:EG13541 , EcoliWiki:b0615 , ModBase:P75726 , OU-Microarray:b0615 , PortEco:citF , Pride:P75726 , Protein Model Portal:P75726 , RefSeq:NP_415148 , RegulonDB:G6341 , SMR:P75726 , String:511145.b0615 , Swiss-Model:P75726 , UniProt:P75726

Relationship Links: InterPro:IN-FAMILY:IPR006472 , Pfam:IN-FAMILY:PF04223

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006084 - acetyl-CoA metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0008814 - citrate CoA-transferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0008815 - citrate (pro-3S)-lyase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0009346 - citrate lyase complex Inferred by computational analysis [GOA01a]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Essentiality data for citF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: citrate lyase, inactive

Synonyms: (HS)-citrate lyase, citrate lyase, thiol form, deacetyl-citrate lyase

Subunit composition of citrate lyase, inactive = [(CitD)6][(CitF)6][(CitE)6]
         citrate lyase [acyl carrier protein] component = (CitD)6 (summary available)
                 citrate lyase, acyl carrier γ subunit = CitD
         citrate lyase, citrate-ACP transferase component = (CitF)6
                 citrate lyase, citrate-ACP transferase α subunit = CitF
         citrate lyase, citryl-ACP lyase component = (CitE)6
                 citrate lyase, citryl-ACP lyase β subunit = CitE

Reactions known to consume the compound:

citrate lyase activation :
[a holo citrate lyase acyl-carrier protein] + acetate + ATP → an acetyl-[holo citrate lyase acyl-carrier protein] + AMP + diphosphate
[a holo citrate lyase acyl-carrier protein] + S-acetyl phosphopantetheine → an acetyl-[holo citrate lyase acyl-carrier protein] + 4'-phosphopantetheine

Reactions known to produce the compound:

citrate lyase activation :
an acetyl-[holo citrate lyase acyl-carrier protein] + 6 H2O → [a holo citrate lyase acyl-carrier protein] + 6 acetate
2'-(5''-triphospho-α-D-ribosyl)-3'-dephospho-CoA + [an apo citrate-lyase acyl-carrier protein] → [a holo citrate lyase acyl-carrier protein] + diphosphate


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bobon: Bobon J, Parent M, Toussaint C, Pinchard A "[Long-acting neuroleptics. IV. Preliminary study of clopimozide (R 29764)]." Acta Psychiatr Belg 76(1);138-43. PMID: 970182

Ingmer98: Ingmer H, Miller CA, Cohen SN (1998). "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator." Mol Microbiol 29(1);49-59. PMID: 9701802

Scheu12: Scheu PD, Witan J, Rauschmeier M, Graf S, Liao YF, Ebert-Jung A, Basche T, Erker W, Unden G (2012). "CitA/CitB two-component system regulating citrate fermentation in Escherichia coli and its relation to the DcuS/DcuR system in vivo." J Bacteriol 194(3);636-45. PMID: 22101843

Yamamoto08a: Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A (2008). "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli." Biosci Biotechnol Biochem 72(11);3011-4. PMID: 18997424


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.