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Escherichia coli K-12 substr. MG1655 Protein: GspC-O secreton complex

Subunit composition of GspC-O secreton complex = [GspC][GspD][GspE][GspF][GspG][GspH][GspI][GspJ][GspK][GspL][GspM][GspO]
         putative protein secretion protein for export = GspC (summary available)
         putative protein secretion protein for export = GspD (summary available)
         putative protein secretion protein for export = GspE (extended summary available)
         putative protein secretion protein for export = GspF (summary available)
         putative protein secretion protein for export = GspG (summary available)
         putative protein secretion protein for export = GspH (summary available)
         putative protein secretion protein for export = GspI (summary available)
         putative protein secretion protein for export = GspJ (summary available)
         putative protein secretion protein for export = GspK (summary available)
         putative protein secretion protein for export = GspL (summary available)
         putative protein secretion protein = GspM (summary available)
         leader peptidase, integral membrane protein = GspO (extended summary available)

Summary:
GspC-O, the type II secretion or secreton complex in Escherichia coli has also been known as the general secretory pathway (GSP) for the export of proteins across the outer membranes of gram-negative bacteria [Pugsley93]. Translocation across the cytoplasmic membrane prior to secreton-dependent secretion can take place via the signal peptide-dependent Sec pathway or the twin-arginine translocation (TAT) system [Voulhoux01]. After transport across the outer membrane, the translocated proteins may be either released into the medium or stay attached to the outer membrane and assembled into surface organelles [Pugsley93, Pugsley98]. The secreton facilitates the extrusion of folded proteins through a putative large gated pore in the outer membrane [Pugsley93]. Although Escherichia coli K-12 does not secrete endogenous proteins, the gsp genes of E. coli are orthologs of those in other secretons [Francetic96], including those of the pullulanase (pul) secretion pathway of Klebsiella oxytoca. The pulO gene product has been shown to be an enzyme required for processing of PulG as well as PulH, PulI and PulJ [Pugsley93a, Strom94]. Complementation studies have shown that the pulO homolog, gspO, is capable of complementing the processing function in a pulO deletion mutant [Francetic96]. The pulG homolog, gspG, has been shown to be capable of complementing a pulG mutation when expressed under the control of a lac promoter [Francetic96]. Transcription of the gspC-O operon was shown to be silenced in wild-type E. coli K-12 by the nucleoid structuring protein H-NS. Deletion mutants lacking H-NS and with the gsp genes present on a multiple-copy-number plasmid have been shown to express the secreton genes and promote the efficient secretion of the co-regulated endochitinase ChiA [Francetic00]. Expression of gspC-O also resulted in formation of pili composed of the GspG pseudopilin when extra copies of gspG were supplied on a second plasmid [Vignon03].

Gene-Reaction Schematic: ?


Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheE, GspC

Gene: gspC Accession Numbers: G7702 (EcoCyc), b3324, ECK3311

Locations: inner membrane

Sequence Length: 271 AAs

Molecular Weight: 30.038 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3324 , Protein Model Portal:P45757 , RefSeq:NP_417783 , SMR:P45757 , String:511145.b3324 , UniProt:P45757

Relationship Links: InterPro:IN-FAMILY:IPR001478 , InterPro:IN-FAMILY:IPR001639 , Prosite:IN-FAMILY:PS01141

Summary:
In Escherichia coli, gspC is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. GspC has been shown, in coimmunoprecipitation studies, to interact with the cytoplasmic membrane through the formation of heterooligomers with GspE, GspL and GspM [Py01, Possot00, Sandkvist00].

Essentiality data for gspC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheF, GspD

Gene: gspD Accession Numbers: G7703 (EcoCyc), b3325, ECK3312

Locations: outer membrane

Sequence Length: 650 AAs

Molecular Weight: 70.698 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0009306 - protein secretion Inferred by computational analysis [GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0009279 - cell outer membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09]
GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0019867 - outer membrane Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3325 , Protein Model Portal:P45758 , RefSeq:NP_417784 , SMR:P45758 , String:511145.b3325 , UniProt:P45758

Relationship Links: EcoO157Cyc:Homolog:ESCC-MONOMER , EcoO157Cyc:Homolog:Z4197-MONOMER , InterPro:IN-FAMILY:IPR001775 , InterPro:IN-FAMILY:IPR004845 , InterPro:IN-FAMILY:IPR004846 , InterPro:IN-FAMILY:IPR005644 , InterPro:IN-FAMILY:IPR013356 , Pfam:IN-FAMILY:PF00263 , Pfam:IN-FAMILY:PF03958 , Prints:IN-FAMILY:PR00811 , Prosite:IN-FAMILY:PS00875

Summary:
In Escherichia coli, gspD is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. GspD belongs to a large family of homologous proteins called secretins, some of which are also involved in type III, type IV and filamentous phage extrusion [Genin94]. 12-14 subunits of GspD are thought to form an oligomeric pore in the outer membrane [Bitter98, Kazmierczak94, Hardie96]. The C-terminal outer membrane domain is conserved among secretins while the N-terminus is variable and may interact with other secretion apparatus components in the periplasm [Sandkvist01].

gspD is not required for spontaneous plasmid transformation on nutrient plates with high agar concentration [Sun09].

Essentiality data for gspD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheG, GspE

Gene: gspE Accession Numbers: G7704 (EcoCyc), b3326, ECK3313

Locations: cytosol

Sequence Length: 493 AAs

Molecular Weight: 54.611 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Arifuzzaman06]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3326 , ModBase:P45759 , Protein Model Portal:P45759 , RefSeq:NP_417785 , SMR:P45759 , String:511145.b3326 , Swiss-Model:P45759 , UniProt:P45759

Relationship Links: InterPro:IN-FAMILY:IPR001482 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR013369 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00437 , Prosite:IN-FAMILY:PS00662 , Smart:IN-FAMILY:SM00382

Summary:
In Escherichia coli, gspE is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. GspE, associated with the cytoplasmic side of the inner membrane [Sandkvist01], has been shown, in coimmunoprecipitation studies, to interact through the formation of heterooligomers with GspC, GspL and GspM [Py01], [Possot00], [Sandkvist00]. Products of gspE orthologs have been shown to contain motifs commonly found in ATP-binding proteins which are critical for translocation across the outer membrane [Sandkvist95] and fusion studies have demonstrated that the orthologs possess ATPase activity [Camberg05]. Taken together, these results suggest that GspE is a cytoplasmic ATPase which couples energy to the type II apparatus to enable secretion.

Essentiality data for gspE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: HopF, GspF, HofF

Gene: gspF Accession Numbers: G7705 (EcoCyc), b3327, ECK3314

Locations: inner membrane

Sequence Length: 398 AAs

Molecular Weight: 44.339 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0009306 - protein secretion Inferred by computational analysis [GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05, Thomas97a]
GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun cell structure membrane
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3327 , Protein Model Portal:P41441 , RefSeq:NP_417786 , SMR:P41441 , String:511145.b3327 , UniProt:P41441

Relationship Links: InterPro:IN-FAMILY:IPR001992 , InterPro:IN-FAMILY:IPR003004 , InterPro:IN-FAMILY:IPR011850 , InterPro:IN-FAMILY:IPR018076 , Pfam:IN-FAMILY:PF00482 , Prints:IN-FAMILY:PR00812 , Prosite:IN-FAMILY:PS00874

Summary:
In Escherichia coli, gspF is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. No interactions with other secreton proteins has been determined however topology analyses of orthologs suggest that GspF is an integral membrane protein [Thomas97a].

Essentiality data for gspF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: HopG, GspG, HofG

Gene: gspG Accession Numbers: G7706 (EcoCyc), b3328, ECK3315

Sequence Length: 145 AAs

Molecular Weight: 15.905 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3328 , Protein Model Portal:P41442 , RefSeq:NP_417787 , SMR:P41442 , String:511145.b3328 , Swiss-Model:P41442 , UniProt:P41442

Relationship Links: InterPro:IN-FAMILY:IPR000983 , InterPro:IN-FAMILY:IPR010054 , InterPro:IN-FAMILY:IPR012902 , InterPro:IN-FAMILY:IPR013545 , Pfam:IN-FAMILY:PF08334 , Pfam:IN-FAMILY:PF13544 , Prints:IN-FAMILY:PR00813 , Prosite:IN-FAMILY:PS00409

Summary:
In Escherichia coli, gspG is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. The GspG, H, I, J and K proteins are similar to type IV pilus subunits [Nunn93], [Bleves98] and are referred to as pseudopilins [Okabe00]. The GspG ortholog, PulG, has been shown to constitute the major component of a pilus-like bundle when overexpressed on high copy-number plasmids [Okabe00].

Essentiality data for gspG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: HopH, GspH, HofH

Gene: gspH Accession Numbers: G7707 (EcoCyc), b3329, ECK3316

Sequence Length: 169 AAs

Molecular Weight: 18.565 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3329 , Pride:P41443 , Protein Model Portal:P41443 , RefSeq:NP_417788 , SMR:P41443 , String:511145.b3329 , UniProt:P41443

Relationship Links: InterPro:IN-FAMILY:IPR002416 , InterPro:IN-FAMILY:IPR012902 , InterPro:IN-FAMILY:IPR022346 , PDB:Structure:2KNQ , Pfam:IN-FAMILY:PF12019 , Pfam:IN-FAMILY:PF13544 , Prints:IN-FAMILY:PR00885 , Prosite:IN-FAMILY:PS00409

Summary:
In Escherichia coli, gspH is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. The GspG, H, I, J and K proteins are similar to type IV pilus subunits [Nunn93], [Bleves98] and are referred to as pseudopilins [Okabe00]. Studies using orthologous type II secretion systems suggest that GspH is dispensable for secretion [Possot00].

Essentiality data for gspH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheH, GspI

Gene: gspI Accession Numbers: G7708 (EcoCyc), b3330, ECK3317

Sequence Length: 125 AAs

Molecular Weight: 13.901 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3330 , Protein Model Portal:P45760 , RefSeq:NP_417789 , SMR:P45760 , String:511145.b3330 , UniProt:P45760

Relationship Links: InterPro:IN-FAMILY:IPR003413 , InterPro:IN-FAMILY:IPR010052 , InterPro:IN-FAMILY:IPR012902 , Pfam:IN-FAMILY:PF02501 , Prosite:IN-FAMILY:PS00409

Summary:
In Escherichia coli, gspI is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. The GspG, H, I, J and K proteins are similar to type IV pilus subunits [Nunn93], [Bleves98] and are referred to as pseudopilins [Okabe00]. Deletion mutation studies showed that the pseudopilins (with the exception of GspH) are required for protein secretion [Possot00].

Essentiality data for gspI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheI, GspJ

Gene: gspJ Accession Numbers: G7709 (EcoCyc), b3331, ECK3318

Locations: inner membrane

Sequence Length: 195 AAs

Molecular Weight: 22.231 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
GO:0015628 - protein secretion by the type II secretion system Inferred by computational analysis [GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]
GO:0015627 - type II protein secretion system complex Inferred by computational analysis [GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3331 , Pride:P45761 , Protein Model Portal:P45761 , RefSeq:NP_417790 , SMR:P45761 , String:511145.b3331 , UniProt:P45761

Relationship Links: InterPro:IN-FAMILY:IPR010055 , InterPro:IN-FAMILY:IPR012902 , Pfam:IN-FAMILY:PF11612 , Pfam:IN-FAMILY:PF13544 , Prosite:IN-FAMILY:PS00409

Summary:
In Escherichia coli, gspJ is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. The GspG, H, I, J and K proteins are similar to type IV pilus subunits [Nunn93], [Bleves98] and are referred to as pseudopilins [Okabe00]. Deletion mutation studies showed that the pseudopilins (with the exception of GspH) are required for protein secretion [Possot00].

Essentiality data for gspJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheJ, GspK

Gene: gspK Accession Numbers: G7710 (EcoCyc), b3332, ECK3319

Locations: inner membrane

Sequence Length: 327 AAs

Molecular Weight: 37.647 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0009306 - protein secretion Inferred by computational analysis [GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3332 , Pride:P45762 , Protein Model Portal:P45762 , RefSeq:NP_417791 , SMR:P45762 , String:511145.b3332 , UniProt:P45762

Relationship Links: InterPro:IN-FAMILY:IPR005628 , Pfam:IN-FAMILY:PF03934

Summary:
In Escherichia coli, gspK is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. The GspG, H, I, J and K proteins are similar to type IV pilus subunits [Nunn93], [Bleves98] and are referred to as pseudopilins [Okabe00]. Deletion mutation studies showed that the pseudopilins (with the exception of GspH) are required for protein secretion [Possot00].

Essentiality data for gspK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein for export

Synonyms: YheK, GspL

Gene: gspL Accession Numbers: G7711 (EcoCyc), b3333, ECK3320

Locations: cell wall, inner membrane

Sequence Length: 387 AAs

Molecular Weight: 44.54 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0008565 - protein transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Zhang07, Sandkvist01]
GO:0009276 - Gram-negative-bacterium-type cell wall Inferred by computational analysis [GOA01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3333 , Protein Model Portal:P45763 , RefSeq:NP_417792 , SMR:P45763 , String:511145.b3333 , UniProt:P45763

Relationship Links: InterPro:IN-FAMILY:IPR007812 , InterPro:IN-FAMILY:IPR024230 , InterPro:IN-FAMILY:IPR025691 , Pfam:IN-FAMILY:PF05134 , Pfam:IN-FAMILY:PF12693

Summary:
In Escherichia coli, gspL is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. GspL, an inner membrane protein [Sandkvist01], has been shown, in coimmunoprecipitation studies, to interact with GspC, GspE and GspM in the formation of heterooligomers [Py01], [Possot00], [Sandkvist00].

Essentiality data for gspL knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of GspC-O secreton complex: putative protein secretion protein

Synonyms: HopZ, GspM, PshM

Gene: gspM Accession Numbers: EG12173 (EcoCyc), b3334, ECK3321

Locations: inner membrane

Sequence Length: 153 AAs

Molecular Weight: 17.234 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006858 - extracellular transport Inferred by computational analysis [GOA01]
GO:0015031 - protein transport Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Sandkvist01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b3334 , Pride:P36678 , Protein Model Portal:P36678 , RefSeq:NP_417793 , String:511145.b3334 , UniProt:P36678

Relationship Links: InterPro:IN-FAMILY:IPR007690 , InterPro:IN-FAMILY:IPR023229 , Pfam:IN-FAMILY:PF04612

Summary:
In Escherichia coli, gspM is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. GspM, an inner membrane protein [Sandkvist01], has been shown, in coimmunoprecipitation studies, to interact with GspC, GspE and GspL in the formation of heterooligomers [Py01], [Possot00], [Sandkvist00].

GspM homologs have been characterized in Helicobacter pylori [Peck99, Guo01, Bai02, Yamaoka02, Lehours04] and in Erwinia chrysanthemi [Py01]. HopZ of Helicobacter pylori is required for wild-type adhesion [Peck99]. GspM of Erwinia chrysanthemi is an inner membrane protein involved in maturation of the type II secretion complex [Py01].

Essentiality data for gspM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Component enzyme of GspC-O secreton complex : leader peptidase, integral membrane protein

Synonyms: YheC, HopO, HopD, GspO, HofD

Gene: gspO Accession Numbers: EG11359 (EcoCyc), b3335, ECK3322

Locations: inner membrane

Sequence Length: 225 AAs

Molecular Weight: 24.957 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred by computational analysis [UniProtGOA11]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0008233 - peptidase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11, Francetic96]
GO:0004190 - aspartic-type endopeptidase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism degradation of macromolecules proteins/peptides/glycopeptides

Unification Links: EcoliWiki:b3335 , PR:PRO_000022912 , Protein Model Portal:P25960 , RefSeq:NP_417794 , String:511145.b3335 , UniProt:P25960

Relationship Links: InterPro:IN-FAMILY:IPR000045 , InterPro:IN-FAMILY:IPR014032 , Pfam:IN-FAMILY:PF01478 , Prints:IN-FAMILY:PR00864

Catalyzes:
a type IV prepillin + H2O → a cleaved type IV prepillin + a peptide

Summary:
In Escherichia coli, gspO is a member of an operon of genes (gspC-O) which are not normally expressed [Francetic00] but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others [Francetic96]. Although Escherichia coli K-12 does not secrete endogenous proteins, the gsp genes of E. coli are orthologs of those in other secretons [Francetic96], including those of the pullulanase (pul) secretion pathway of Klebsiella oxytoca.

GspO encodes a protein similar to type IV prepilin peptidases, like pulO gene in K. oxytoca . Though it is under the control of a promoter that has very poor basal expression under laboratory growth conditions, when expressed from a stronger promoter, GspO promotes cleavage of prepilin peptidase substrates such as type IV prepilin from Neisseria gonorrhoaea and prePulG as well as PulH, PulI and PulJ from K. oxytoca , referred to as pseudopilins [Francetic96], [Pugsley93a], [Strom94], [Possot00]. Complementation studies have shown that gspO is capable of complementing the processing function in a pulO deletion mutant [Francetic96].

Essentiality data for gspO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

References

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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