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Escherichia coli K-12 substr. MG1655 Enzyme: fructoselysine 3-epimerase

Gene: frlC Accession Numbers: G7724 (EcoCyc), b4474, ECK3360

Synonyms: b3373 (obsolete), yhfP, yhfO, b3372 (obsolete)

Regulation Summary Diagram: ?

Regulation summary diagram for frlC

Subunit composition of fructoselysine 3-epimerase = [FrlC]8
         fructoselysine 3-epimerase = FrlC

Fructoselysine 3-epimerase catalyzes the interconversion of fructoselysine and psicoselysine [Wiame04]. Fructoselysine 3-epimerase activity is undetectable when cells are grown on glucose; stationary phase extract of cells grown on fructoselysine or psicoselysine have an epimerase activity of 2 nmol/min per mg of protein [Wiame04].

FrlC: "fructoselysine" [Wiame02]

Gene Citations: [Wiame05]

Locations: cytosol

Map Position: [3,500,362 -> 3,501,192] (75.44 centisomes, 272°)
Length: 831 bp / 276 aa

Molecular Weight of Polypeptide: 31.169 kD (from nucleotide sequence), 30 kD (experimental) [Wiame04 ]

Molecular Weight of Multimer: 260 kD (experimental) [Wiame04]

Unification Links: ASAP:ABE-0174114 , EchoBASE:EB2747 , EcoGene:EG12910 , EcoliWiki:b4474 , ModBase:P45541 , OU-Microarray:b3372 , PortEco:frlC , Protein Model Portal:P45541 , RefSeq:YP_026213 , RegulonDB:G7724 , SMR:P45541 , String:511145.b4474 , UniProt:P45541

Relationship Links: EcoO157Cyc:Homolog:Z4733 , EcoO157Cyc:Homolog:Z4733-MONOMER , InterPro:IN-FAMILY:IPR013022 , Pfam:IN-FAMILY:PF01261

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0046348 - amino sugar catabolic process Inferred from experiment [Wiame04]
Molecular Function: GO:0016857 - racemase and epimerase activity, acting on carbohydrates and derivatives Inferred from experiment [Wiame04]
GO:0042802 - identical protein binding Inferred from experiment [Wiame04]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for frlC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 3]

Enzymatic reaction of: fructoselysine 3-epimerase

D-fructosyl-L-lysine <=> psicoselysine

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: fructoselysine and psicoselysine degradation

The ratio of fructoselysine to psicoselysine at equilibrium is approximately 3 [Wiame04].

Activators (Unknown Mechanism): Ni2+ [Wiame04]

Inhibitors (Unknown Mechanism): Zn2+ [Wiame04]

Kinetic Parameters:

Km (μM)

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/21/2004 (paley) Merged genes G7725/yhfP into G7724/frlC
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Wiame02: Wiame E, Delpierre G, Collard F, Van Schaftingen E (2002). "Identification of a pathway for the utilization of the Amadori product fructoselysine in Escherichia coli." J Biol Chem 277(45);42523-9. PMID: 12147680

Wiame04: Wiame E, Van Schaftingen E (2004). "Fructoselysine 3-epimerase, an enzyme involved in the metabolism of the unusual Amadori compound psicoselysine in Escherichia coli." Biochem J 378(Pt 3);1047-52. PMID: 14641112

Wiame05: Wiame E, Lamosa P, Santos H, Van Schaftingen E (2005). "Identification of glucoselysine-6-phosphate deglycase, an enzyme involved in the metabolism of the fructation product glucoselysine." Biochem J 392(Pt 2);263-9. PMID: 16153181

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Aug 30, 2015, biocyc11.