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Escherichia coli K-12 substr. MG1655 Protein: MntR-Mn2+ DNA-binding transcriptional dual regulator



Gene: mntR Accession Numbers: G6420 (EcoCyc), b0817, ECK0807

Synonyms: ybiQ

Regulation Summary Diagram: ?

Subunit composition of MntR-Mn2+ DNA-binding transcriptional dual regulator = [(MntR)(Mn2+)]2
         MntR-Mn2+ = (MntR)(Mn2+)

Summary:
MntR, "Mn2+ transport regulator", the primary sensor and transducer of manganese abundance, is a dual regulator that senses manganese and negatively or positively controls the transcription of different genes involved in manganese homeostasis [Patzer01, Waters11].

This protein belongs to the DtxR family. The members of this family have three domains: an N-terminal domain with a helix-turn-helix motif, which binds to DNA; a central domain for dimerization and metal ion binding; and a C-terminal alpha-spectrin SH3-like domain. However, MntR lacks the SH3-like domain [Patzer01].

The 3D structure of apo-MntR has been solved. The protein dimerizes via its C-terminal domain. The N-terminal and C-terminal domains are connected by a long α-helical linker [Tanaka09]. The N-terminal domain is expected to bind two manganese ions per monomer, in analogy to BsMntR from B. subtilis, which has been crystallized with and without bound manganese [Glasfeld03, DeWitt07]. All residues involved in manganese binding in BsMntR ar conserved in MntR [Tanaka09].

MntR does not seem to be autoregulated [Patzer01].

Many iron acquisition genes are derepressed in the mntR mutant [Waters11].

Map Position: [852,406 -> 852,873] (18.37 centisomes)
Length: 468 bp / 155 aa

Unification Links: ASAP:ABE-0002793 , EchoBASE:EB3106 , EcoGene:EG13322 , OU-Microarray:b0817 , PortEco:mntR , RegulonDB:G6420

In Reactions of unknown directionality:

Not in pathways:
MntR + Mn2+ = MntR-Mn2+

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

MultiFun Terms: information transfer RNA related Transcription related
regulation type of regulation transcriptional level

DNA binding site length: 22 base-pairs

Symmetry: Inverted Repeat

Consensus DNA Binding Sequence: AAACATAGCaaagGCTATGTTT

Regulated Transcription Units (4 total): ?

Notes:

Credits:
Created 07-Mar-2008 by Gama-Castro S , UNAM
Last-Curated ? 29-May-2008 by Gama-Castro S , UNAM


Subunit of MntR-Mn2+: MntR

Synonyms: YbiQ

Gene: mntR Accession Numbers: G6420 (EcoCyc), b0817, ECK0807

Locations: cytosol

Sequence Length: 155 AAs

Molecular Weight: 17.64 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0045892 - negative regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [Patzer01]
GO:2000144 - positive regulation of DNA-templated transcription, initiation Inferred by computational analysis Inferred from experiment [Waters11]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [GOA01, Patzer01]
GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Rajagopala12, Butland05]
GO:0030145 - manganese ion binding Inferred from experiment [Patzer01, Tanaka09]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0046914 - transition metal ion binding Inferred by computational analysis [GOA01]
GO:0046983 - protein dimerization activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation type of regulation transcriptional level

Unification Links: DIP:DIP-28083N , EcoliWiki:b0817 , ModBase:P0A9F1 , PR:PRO_000023259 , Protein Model Portal:P0A9F1 , RefSeq:NP_415338 , SMR:P0A9F1 , String:511145.b0817 , UniProt:P0A9F1

Relationship Links: InterPro:IN-FAMILY:IPR001367 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR022687 , InterPro:IN-FAMILY:IPR022689 , PDB:Structure:2H09 , Pfam:IN-FAMILY:PF01325 , Pfam:IN-FAMILY:PF02742 , Prosite:IN-FAMILY:PS50944 , Smart:IN-FAMILY:SM00529

In Reactions of unknown directionality:

Not in pathways:
MntR + Mn2+ = MntR-Mn2+

Essentiality data for mntR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

DeWitt07: DeWitt MA, Kliegman JI, Helmann JD, Brennan RG, Farrens DL, Glasfeld A (2007). "The conformations of the manganese transport regulator of Bacillus subtilis in its metal-free state." J Mol Biol 365(5);1257-65. PMID: 17118401

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Glasfeld03: Glasfeld A, Guedon E, Helmann JD, Brennan RG (2003). "Structure of the manganese-bound manganese transport regulator of Bacillus subtilis." Nat Struct Biol 10(8);652-7. PMID: 12847518

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Patzer01: Patzer SI, Hantke K (2001). "Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding an NRAMP-like Mn(2+) transporter in Escherichia coli." J Bacteriol 183(16);4806-13. PMID: 11466284

Rajagopala12: Rajagopala SV, Sikorski P, Caufield JH, Tovchigrechko A, Uetz P (2012). "Studying protein complexes by the yeast two-hybrid system." Methods 58(4);392-9. PMID: 22841565

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Tanaka09: Tanaka T, Shinkai A, Bessho Y, Kumarevel T, Yokoyama S (2009). "Crystal structure of the manganese transport regulatory protein from Escherichia coli." Proteins 77(3);741-6. PMID: 19701940

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Waters11: Waters LS, Sandoval M, Storz G (2011). "The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis." J Bacteriol 193(21);5887-97. PMID: 21908668

Other References Related to Gene Regulation

Raghavan11: Raghavan R, Sage A, Ochman H (2011). "Genome-wide identification of transcription start sites yields a novel thermosensing RNA and new cyclic AMP receptor protein-regulated genes in Escherichia coli." J Bacteriol 193(11);2871-4. PMID: 21460078

Vakulskas14: Vakulskas CA, Pannuri A, Cortes-Selva D, Zere TR, Ahmer BM, Babitzke P, Romeo T (2014). "Global effects of the DEAD-box RNA helicase DeaD (CsdA) on gene expression over a broad range of temperatures." Mol Microbiol 92(5);945-58. PMID: 24708042


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.