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Escherichia coli K-12 substr. MG1655 Transporter: SdsRQP multidrug efflux system

Superclasses: a protein complex
a transporter


Subunit composition of SdsRQP multidrug efflux system = [MdtP][MdtO][MdtN]
         SdsRQP multidrug efflux transport system - predicted outer membrane factor = MdtP (summary available)
         SdsRQP multidrug efflux transport system - uncharacterised component = MdtO (summary available)
         SdsRQP multidrug efflux transport system - predicted membrane fusion protein = MdtN (summary available)

Summary:
SdsRQP is a putative multidrug efflux pump involved in sulfur drug resistance [Shimada09]. An E.coli yjcR null mutant is more sensitive to sulfur drugs than wild type [Shimada09].

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Biological Process:
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Molecular Function:
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]

Credits:
Created 07-Jul-2009 by Mackie A, Macquarie University


Enzymatic reaction of: SdsRQP multidrug efflux pump (SdsRQP multidrug efflux system)

Inferred from experiment

Transport reaction diagram for SdsRQP multidrug efflux pump


Subunit of SdsRQP multidrug efflux system: SdsRQP multidrug efflux transport system - predicted outer membrane factor

Synonyms: MdtP, YjcP, SdsP

Gene: mdtP Accession Numbers: EG11952 (EcoCyc), b4080, ECK4073

Locations: outer membrane

Sequence Length: 488 AAs

Molecular Weight: 53.453 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Inferred from experimentGO:1902599 - sulfathiazole transmembrane transport [Shimada09]
Inferred by computational analysisGO:0006810 - transport [GOA01a]
Inferred by computational analysisGO:0046677 - response to antibiotic [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0008289 - lipid binding [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0009279 - cell outer membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell processesprotectiondrug resistance/sensitivity
transportElectrochemical potential driven transportersPorters (Uni-, Sym- and Antiporters)

Unification Links: EcoliWiki:b4080, ModBase:P32714, PR:PRO_000023196, Pride:P32714, Protein Model Portal:P32714, RefSeq:NP_418504, SMR:P32714, UniProt:P32714

Relationship Links: InterPro:IN-FAMILY:IPR003423, InterPro:IN-FAMILY:IPR010131, Pfam:IN-FAMILY:PF02321, Prosite:IN-FAMILY:PS51257

Summary:
Based on sequence analysis SdsP is an outer membrane factor (OMF) family component of the SdsRQP multidrug efflux pump involved in sulfur drug resistance [Sulavik01, Shimada09].

Deletion of sdsP from strain W3110 resulted in increased susceptibility to acriflavin, puromycin, and tetraphenylarsonium chloride [Sulavik01].

Essentiality data for mdtP knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of SdsRQP multidrug efflux system: SdsRQP multidrug efflux transport system - uncharacterised component

Synonyms: MdtO, YjcQ, SdsQ

Gene: mdtO Accession Numbers: EG11953 (EcoCyc), b4081, ECK4074

Locations: inner membrane

Sequence Length: 683 AAs

Molecular Weight: 76.151 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Inferred from experimentGO:1902599 - sulfathiazole transmembrane transport [Shimada09]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0046677 - response to antibiotic [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Rajagopala14, Butland05]
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, GOA01a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell processesprotectiondrug resistance/sensitivity
transportElectrochemical potential driven transportersPorters (Uni-, Sym- and Antiporters)

Unification Links: DIP:DIP-12556N, EcoliWiki:b4081, Mint:MINT-1312912, PR:PRO_000023195, Pride:P32715, Protein Model Portal:P32715, RefSeq:NP_418505, UniProt:P32715

Relationship Links: InterPro:IN-FAMILY:IPR006726, Pfam:IN-FAMILY:PF04632

Summary:
SdsQ is an uncharacterised member of the SdsRQP multidrug efflux pump [Shimada09].

Essentiality data for mdtO knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of SdsRQP multidrug efflux system: SdsRQP multidrug efflux transport system - predicted membrane fusion protein

Synonyms: MdtN, YjcR, SdsR

Gene: mdtN Accession Numbers: EG11954 (EcoCyc), b4082, ECK4075

Locations: inner membrane

Sequence Length: 343 AAs

Molecular Weight: 36.908 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentInferred by computational analysisGO:0006855 - drug transmembrane transport [GOA01a, Shimada09]
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Inferred from experimentGO:1902599 - sulfathiazole transmembrane transport [Shimada09]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0046677 - response to antibiotic [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]
Inferred by computational analysisGO:0015238 - drug transmembrane transporter activity [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell processesprotectiondrug resistance/sensitivity
transportElectrochemical potential driven transportersPorters (Uni-, Sym- and Antiporters)

Unification Links: EcoliWiki:b4082, ModBase:P32716, PR:PRO_000023194, Protein Model Portal:P32716, RefSeq:NP_418506, SMR:P32716, UniProt:P32716

Relationship Links: InterPro:IN-FAMILY:IPR005694, InterPro:IN-FAMILY:IPR006143, InterPro:IN-FAMILY:IPR030196, Panther:IN-FAMILY:PTHR30367:SF1, Pfam:IN-FAMILY:PF13437, Pfam:IN-FAMILY:PF13533

Summary:
SdsR is predicted to be the membrane fusion protein component of the SdsRQP multidrug efflux pump [Dinh94, Shimada09]. An E. coli yjcR null mutant is more sensitive to sulfur drugs than wild type [Shimada09].

Citations: [Tuntufye12]

Essentiality data for mdtN knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dinh94: Dinh T, Paulsen IT, Saier MH (1994). "A family of extracytoplasmic proteins that allow transport of large molecules across the outer membranes of gram-negative bacteria." J Bacteriol 1994;176(13);3825-31. PMID: 8021163

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Shimada09: Shimada T, Yamamoto K, Ishihama A (2009). "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux." J Bacteriol 191(14);4562-71. PMID: 19429622

Sulavik01: Sulavik MC, Houseweart C, Cramer C, Jiwani N, Murgolo N, Greene J, DiDomenico B, Shaw KJ, Miller GH, Hare R, Shimer G (2001). "Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes." Antimicrob Agents Chemother 45(4);1126-36. PMID: 11257026

Tuntufye12: Tuntufye HN, Lebeer S, Gwakisa PS, Goddeeris BM (2012). "Identification of Avian pathogenic Escherichia coli genes that are induced in vivo during infection in chickens." Appl Environ Microbiol 78(9);3343-51. PMID: 22344666

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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