Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: p-aminobenzoyl-glutamate hydrolase


Subunit composition of p-aminobenzoyl-glutamate hydrolase = [AbgB]2[AbgA]2
         p-aminobenzoyl-glutamate hydrolase subunit B = AbgB (summary available)
         p-aminobenzoyl-glutamate hydrolase subunit A = AbgA (summary available)

Summary:
p-aminobenzoyl-glutamate hydrolase (PGH) may be involved in the utilization of p-aminobenzoyl-glutamate, the major end product of folate catabolism in humans [Green10].

Mutations that appear to enhance expression of abgABT allow a pabA mutant to utilize p-aminobenzoyl-glutamate as a source of p-aminobenzoate for folate biosynthesis [Hussein98]. The abgAB genes are not essential for utilization of p-aminobenzoyl-glutamate; thus, there may be a second PGH activity present in E. coli [Hussein98].

Molecular Weight: 206.0 kD (experimental) [Green10]

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Molecular Function:
Inferred from experimentGO:0071713 - para-aminobenzoyl-glutamate hydrolase activity [Green10]

Credits:
Created 08-Mar-2010 by Keseler I, SRI International
Last-Curated 08-Mar-2010 by Keseler I, SRI International


Enzymatic reaction of: p-aminobenzoyl-glutamate hydrolase

Inferred from experiment

Synonyms: PGH

EC Number: 3.5.1.-

p-aminobenzoyl glutamate + H2O → 4-aminobenzoate + L-glutamate

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown. [Green10]

Cofactors or Prosthetic Groups: Mn2+ [Green10]

Kinetic Parameters:
Substrate Km (μM) Citations
p-aminobenzoyl glutamate 60.0 [Green10]

Subunit of p-aminobenzoyl-glutamate hydrolase: p-aminobenzoyl-glutamate hydrolase subunit B

Synonyms: YdaI, AbgB

Gene: abgB Accession Numbers: G6669 (EcoCyc), b1337, ECK1333

Locations: cytosol

Sequence Length: 481 AAs

Molecular Weight: 52.194 kD (from nucleotide sequence)

Molecular Weight: 53.0 kD (experimental) [Green10]


GO Terms:
Biological Process:
Inferred from experimentGO:0046657 - folic acid catabolic process [Hussein98]
Molecular Function:
Inferred from experimentGO:0046982 - protein heterodimerization activity [Green10]
Inferred from experimentGO:0071713 - para-aminobenzoyl-glutamate hydrolase activity [Green10, Carter07]
Inferred by computational analysisGO:0016787 - hydrolase activity [UniProtGOA11a, GOA01a]
Cellular Component:
Inferred from experimentGO:0005737 - cytoplasm [Green10, Bartsch90]
Inferred by computational analysisGO:0005829 - cytosol [DiazMejia09]

MultiFun Terms: metabolismmetabolism of other compounds

Unification Links: DIP:DIP-9029N, EcoliWiki:b1337, ModBase:P76052, PR:PRO_000022028, Pride:P76052, Protein Model Portal:P76052, RefSeq:NP_415853, SMR:P76052, UniProt:P76052

Relationship Links: InterPro:IN-FAMILY:IPR011650, InterPro:IN-FAMILY:IPR017145, InterPro:IN-FAMILY:IPR017439, Pfam:IN-FAMILY:PF01546

Summary:
AbgB: "p-aminobenzoyl-glutamate utilization"

Essentiality data for abgB knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of p-aminobenzoyl-glutamate hydrolase: p-aminobenzoyl-glutamate hydrolase subunit A

Synonyms: YdaJ, AbgA

Gene: abgA Accession Numbers: G6670 (EcoCyc), b1338, ECK1334

Locations: cytosol

Sequence Length: 436 AAs

Molecular Weight: 46.588 kD (from nucleotide sequence)

Molecular Weight: 47.0 kD (experimental) [Green10]


GO Terms:
Biological Process:
Inferred from experimentGO:0046657 - folic acid catabolic process [Hussein98]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Molecular Function:
Inferred from experimentGO:0046982 - protein heterodimerization activity [Green10]
Inferred from experimentGO:0071713 - para-aminobenzoyl-glutamate hydrolase activity [Green10, Carter07]
Inferred by computational analysisGO:0016787 - hydrolase activity [UniProtGOA11a, GOA01a]
Cellular Component:
Inferred from experimentGO:0005737 - cytoplasm [Green10]
Inferred by computational analysisGO:0005829 - cytosol [DiazMejia09]

MultiFun Terms: metabolismmetabolism of other compounds

Unification Links: EcoliWiki:b1338, ModBase:P77357, PR:PRO_000022027, Pride:P77357, Protein Model Portal:P77357, RefSeq:NP_415854, SMR:P77357, UniProt:P77357

Relationship Links: InterPro:IN-FAMILY:IPR002933, InterPro:IN-FAMILY:IPR011650, InterPro:IN-FAMILY:IPR017439, Pfam:IN-FAMILY:PF01546, Pfam:IN-FAMILY:PF07687

Summary:
Transcription of the potential abgABT operon may be regulated by the predicted transcriptional regulator AbgR [Hussein98].

AbgA: "p-aminobenzoyl-glutamate utilization" [Hussein98]

Essentiality data for abgA knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bartsch90: Bartsch K, von Johnn-Marteville A, Schulz A (1990). "Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD)." J Bacteriol 1990;172(12);7035-42. PMID: 2254272

Carter07: Carter EL, Jager L, Gardner L, Hall CC, Willis S, Green JM (2007). "Escherichia coli abg genes enable uptake and cleavage of the folate catabolite p-aminobenzoyl-glutamate." J Bacteriol 189(9);3329-34. PMID: 17307853

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Green10: Green JM, Hollandsworth R, Pitstick L, Carter EL (2010). "Purification and characterization of the folate catabolic enzyme p-aminobenzoyl-glutamate hydrolase from Escherichia coli." J Bacteriol 192(9);2407-13. PMID: 20190044

Hussein98: Hussein MJ, Green JM, Nichols BP (1998). "Characterization of mutations that allow p-aminobenzoyl-glutamate utilization by Escherichia coli." J Bacteriol 180(23);6260-8. PMID: 9829935

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Tue May 3, 2016, biocyc13.