Escherichia coli K-12 substr. MG1655 Protein: CreC sensory histidine kinase

Gene: creC Accession Numbers: EG10730 (EcoCyc), b4399, ECK4391

Synonyms: phoM

Regulation Summary Diagram: ?

Regulation summary diagram for creC

Subunit composition of CreC sensory histidine kinase = [CreC]2

Alternative forms of CreC sensory histidine kinase: CreC sensory histidine kinase - phosphorylated (summary available)

CreC is the sensor histidine kinase of the CreCB two-component signal transduction system [Ludtke84, Amemura90] which is activated when E.coli is fermenting glycolytic carbon sources and during aerobic growth on gluconeogenic carbon [Avison01, Cariss08]. CreC is a transmembrane protein composed of three domains: external sensory domain (amino-terminal), cytoplasmic transmitter domain (carboxyl-terminal) and transmembrane hydrophobic central domain [Amemura86]. CreC functions as a membrane-associated protein kinase that phosphorylates the transcriptional activator CreB however the specific ligand that activate CreC is not known.

CreC was originally called PhoM as it was shown to act as the the phosphate donor for PhoB ([Wanner80, Makino84, Tommassen84] but this activity is masked in PhoR+ strains and the CreC/PhoB pathway is not responsive to phosphate levels [Wanner80].

Gene Citations: [Nonaka06]

Locations: inner membrane

Map Position: [4,634,719 -> 4,636,143] (99.89 centisomes, 360°)
Length: 1425 bp / 474 aa

Molecular Weight of Polypeptide: 52.176 kD (from nucleotide sequence)

pI: 9.1

Unification Links: ASAP:ABE-0014430 , CGSC:395 , DIP:DIP-9319N , EchoBASE:EB0723 , EcoGene:EG10730 , EcoliWiki:b4399 , Mint:MINT-1307740 , ModBase:P08401 , OU-Microarray:b4399 , PortEco:creC , PR:PRO_000022330 , Pride:P08401 , Protein Model Portal:P08401 , RegulonDB:EG10730 , SMR:P08401 , String:511145.b4399 , UniProt:P08401

Relationship Links: InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003660 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , InterPro:IN-FAMILY:IPR029151 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF00672 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Prosite:IN-FAMILY:PS50885 , Smart:IN-FAMILY:SM00304 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

CreCB Two-Component Signal Transduction System :
ATP + CreC → ADP + CreC sensory histidine kinase - phosphorylated

Reactions known to produce the compound:

CreCB Two-Component Signal Transduction System :
CreC sensory histidine kinase - phosphorylated + CreB → CreB-Pasp54 + CreC

In Reactions of unknown directionality:

Not in pathways:
CreC sensory histidine kinase - phosphorylated + PhoB = CreC + PhoB-Pasp53

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for creC

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Amemura90]
GO:0023014 - signal transduction by protein phosphorylation Inferred from experiment Inferred by computational analysis [GOA01a, Amemura90]
GO:0007165 - signal transduction Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0018106 - peptidyl-histidine phosphorylation Inferred by computational analysis [Gaudet10]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment Inferred by computational analysis [GOA01a, Amemura90]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005622 - intracellular Inferred by computational analysis Inferred from experiment [Amemura90, UniProtGOA11a]
GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Amemura90]
GO:0016021 - integral component of membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Amemura90]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a, Amemura86]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for creC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of CreC with features indicated

Feature Class Location Citations Comment State
Transmembrane-Region 7 -> 27
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 77
[Amemura86, UniProt10a]
UniProt: (in Ref. 1; AAA24375);
Transmembrane-Region 147 -> 167
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 184 -> 204
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 205 -> 255
UniProt: HAMP;
Conserved-Region 262 -> 473
UniProt: Histidine kinase;
Phosphorylation-Modification 265
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b4399 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10730; confirmed by SwissProt match.


Amemura86: Amemura M, Makino K, Shinagawa H, Nakata A (1986). "Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon." J Bacteriol 1986;168(1);294-302. PMID: 3531171

Amemura90: Amemura M, Makino K, Shinagawa H, Nakata A (1990). "Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2." J Bacteriol 1990;172(11);6300-7. PMID: 2228961

Avison01: Avison MB, Horton RE, Walsh TR, Bennett PM (2001). "Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media." J Biol Chem 276(29);26955-61. PMID: 11350954

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cariss08: Cariss SJ, Tayler AE, Avison MB (2008). "Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli." J Bacteriol 190(11);3930-9. PMID: 18375564

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ludtke84: Ludtke D, Bernstein J, Hamilton C, Torriani A (1984). "Identification of the phoM gene product and its regulation in Escherichia coli K-12." J Bacteriol 159(1);19-25. PMID: 6330029

Makino84: Makino K, Shinagawa H, Nakata A (1984). "Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli." Mol Gen Genet 195(3);381-90. PMID: 6381964

Nonaka06: Nonaka G, Blankschien M, Herman C, Gross CA, Rhodius VA (2006). "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress." Genes Dev 20(13);1776-89. PMID: 16818608

Tommassen84: Tommassen J, Heimstra P, Overduin P, Lugtenberg B (1984). "Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12." Mol Gen Genet 195(1-2);190-4. PMID: 6092847

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wanner80: Wanner BL, Latterell P (1980). "Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli." Genetics 96(2);353-66. PMID: 7021308

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Gene Regulation

Marzan13: Marzan LW, Hasan CM, Shimizu K (2013). "Effect of acidic condition on the metabolic regulation of Escherichia coli and its phoB mutant." Arch Microbiol 195(3);161-71. PMID: 23274360

Oberto10: Oberto J (2010). "FITBAR: a web tool for the robust prediction of prokaryotic regulons." BMC Bioinformatics 11;554. PMID: 21070640

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc12.