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discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein



Gene: aroM Accession Numbers: EG10083 (EcoCyc), b0390, ECK0385

Regulation Summary Diagram: ?

Summary:
AroM is encoded in an operon together with AroL, shikimate kinase II [DeFeyter86a, DeFeyter86b]. AroM is not required for growth on minimal medium, and its function is unknown [DeFeyter86a].

Locations: cytosol

Map Position: [406,652 -> 407,329] (8.76 centisomes)
Length: 678 bp / 225 aa

Molecular Weight of Polypeptide: 24.917 kD (from nucleotide sequence), 26 kD (experimental) [DeFeyter86a ]

Unification Links: ASAP:ABE-0001357 , CGSC:18523 , EchoBASE:EB0081 , EcoGene:EG10083 , EcoliWiki:b0390 , OU-Microarray:b0390 , PortEco:aroM , PR:PRO_000022152 , Protein Model Portal:P0AE28 , RefSeq:NP_414924 , RegulonDB:EG10083 , String:511145.b0390 , UniProt:P0AE28

Relationship Links: InterPro:IN-FAMILY:IPR010843 , Pfam:IN-FAMILY:PF07302 , ProDom:IN-FAMILY:PD037589

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0008652 - cellular amino acid biosynthetic process Inferred by computational analysis [UniProtGOA11]
GO:0009073 - aromatic amino acid family biosynthetic process Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs Conserved-ORFs

Essentiality data for aroM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Curated 30-Jan-2006 by Shearer A , SRI International
Last-Curated ? 21-Jul-2008 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 152
[DeFeyter86b, UniProt10a]
Alternate sequence: L → W; UniProt: (in Ref. 1; AAA83835);
Sequence-Conflict 155
[DeFeyter86b, UniProt10a]
Alternate sequence: S → R; UniProt: (in Ref. 1; AAA83835);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0390 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10083; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DeFeyter86a: DeFeyter RC, Pittard J (1986). "Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12." J Bacteriol 1986;165(1);226-32. PMID: 3001024

DeFeyter86b: DeFeyter RC, Davidson BE, Pittard J (1986). "Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12." J Bacteriol 1986;165(1);233-9. PMID: 3001025

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Chung13: Chung D, Park D, Myers K, Grass J, Kiley P, Landick R, Keles S (2013). "dPeak: high resolution identification of transcription factor binding sites from PET and SET ChIP-Seq data." PLoS Comput Biol 9(10);e1003246. PMID: 24146601

Heatwole92: Heatwole VM, Somerville RL (1992). "Synergism between the Trp repressor and Tyr repressor in repression of the aroL promoter of Escherichia coli K-12." J Bacteriol 1992;174(1);331-5. PMID: 1530846

Jeeves99: Jeeves M, Evans PD, Parslow RA, Jaseja M, Hyde EI (1999). "Studies of the Escherichia coli Trp repressor binding to its five operators and to variant operator sequences." Eur J Biochem 1999;265(3);919-28. PMID: 10518785

Lawley94: Lawley B, Pittard AJ (1994). "Regulation of aroL expression by TyrR protein and Trp repressor in Escherichia coli K-12." J Bacteriol 176(22);6921-30. PMID: 7961453

Millar86: Millar G, Lewendon A, Hunter MG, Coggins JR (1986). "The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product." Biochem J 1986;237(2);427-37. PMID: 3026317

Yang96: Yang J, Gunasekera A, Lavoie TA, Jin L, Lewis DE, Carey J (1996). "In vivo and in vitro studies of TrpR-DNA interactions." J Mol Biol 258(1);37-52. PMID: 8613990

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.