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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted anaerobic sulfatase maturation enzyme



Gene: aslB Accession Numbers: EG10090 (EcoCyc), b3800, ECK3793

Synonyms: gppB, atsB

Regulation Summary Diagram: ?

Summary:
AslB is a member of the anaerobic sulfatase maturation enzyme subfamily of the Radical SAM superfamily of enzymes [Sofia01, Berteau06].

Both an aslB single and aslB ydeM double null mutant is still able to mature a heterologous sulfatase from Clostridium perfringens. Maturation of the C. perfringens enzyme appears to be dependent on a different enzyme that is present only under aerobic conditions [Benjdia07].

Expression of aslB is activated by the transcriptional regulator Rob [Bennik00].

Locations: cytosol

Map Position: [3,980,981 -> 3,982,216] (85.8 centisomes)
Length: 1236 bp / 411 aa

Molecular Weight of Polypeptide: 46.513 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012408 , CGSC:33960 , EchoBASE:EB0088 , EcoGene:EG10090 , EcoliWiki:b3800 , ModBase:P25550 , OU-Microarray:b3800 , PortEco:aslB , Pride:P25550 , Protein Model Portal:P25550 , RefSeq:YP_026259 , RegulonDB:EG10090 , SMR:P25550 , String:511145.b3800 , UniProt:P25550

Relationship Links: InterPro:IN-FAMILY:IPR007197 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR023867 , InterPro:IN-FAMILY:IPR023885 , Pfam:IN-FAMILY:PF04055 , Pfam:IN-FAMILY:PF13186

In Paralogous Gene Group: 551 (2 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0051604 - protein maturation Inferred by computational analysis [Berteau06]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [GOA01]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0051539 - 4 iron, 4 sulfur cluster binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related chaperoning, repair (refolding)

Essentiality data for aslB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 26-Feb-2007 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 21
[UniProt10]
UniProt: Iron-sulfur 1 (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: potential;
Metal-Binding-Site 25
[UniProt10]
UniProt: Iron-sulfur 1 (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: potential;
Metal-Binding-Site 28
[UniProt10]
UniProt: Iron-sulfur 1 (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: potential;
Sequence-Conflict 116 -> 117
[Daniels92, UniProt10a]
Alternate sequence: CA → WP; UniProt: (in Ref. 2; AAA67596);
Metal-Binding-Site 276
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 282
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 297
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 339
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 342
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 348
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Metal-Binding-Site 352
[UniProt10]
UniProt: Iron-sulfur; Non-Experimental Qualifier: potential;
Sequence-Conflict 391
[Daniels92, UniProt10a]
Alternate sequence: L → C; UniProt: (in Ref. 2; AAA67596);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3800 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10090; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Benjdia07: Benjdia A, Deho G, Rabot S, Berteau O (2007). "First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants." FEBS Lett 581(5):1009-14. PMID: 17303125

Bennik00: Bennik MH, Pomposiello PJ, Thorne DF, Demple B (2000). "Defining a rob regulon in Escherichia coli by using transposon mutagenesis." J Bacteriol 182(13);3794-801. PMID: 10850996

Berteau06: Berteau O, Guillot A, Benjdia A, Rabot S (2006). "A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes." J Biol Chem 281(32);22464-70. PMID: 16766528

Daniels92: Daniels DL, Plunkett G, Burland V, Blattner FR (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 1992;257(5071);771-8. PMID: 1379743

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sofia01: Sofia HJ, Chen G, Hetzler BG, Reyes-Spindola JF, Miller NE (2001). "Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods." Nucleic Acids Res 29(5);1097-106. PMID: 11222759

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

Krin10: Krin E, Danchin A, Soutourina O (2010). "Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli." BMC Microbiol 10;273. PMID: 21034467

Zhao10a: Zhao K, Liu M, Burgess RR (2010). "Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis." Nucleic Acids Res 38(4);1273-83. PMID: 19969540


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14A.