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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted flagella assembly protein



Gene: flk Accession Numbers: EG10229 (EcoCyc), b2321, ECK2315

Synonyms: div

Regulation Summary Diagram: ?

Regulation summary diagram for flk

Summary:
No information about this protein was found by a literature search conducted on February 1, 2006.

Gene Citations: [Schoenlein89]

Locations: inner membrane

Map Position: [2,435,972 -> 2,436,967] (52.5 centisomes, 189°)
Length: 996 bp / 331 aa

Molecular Weight of Polypeptide: 36.668 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007667 , DIP:DIP-9448N , EchoBASE:EB0225 , EcoGene:EG10229 , EcoliWiki:b2321 , OU-Microarray:b2321 , PortEco:flk , PR:PRO_000022672 , Protein Model Portal:P15286 , RefSeq:NP_416824 , RegulonDB:EG10229 , String:511145.b2321 , UniProt:P15286

Relationship Links: InterPro:IN-FAMILY:IPR023597

GO Terms:

Biological Process: GO:0010468 - regulation of gene expression Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]
GO:0031224 - intrinsic component of membrane Inferred by computational analysis [GOA01]

MultiFun Terms: cell structure flagella

Essentiality data for flk knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 01-Feb-2006 by Shearer A , SRI International


Sequence Features

Protein sequence of predicted flagella assembly protein with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 2
[Schoenlein89, UniProt10a]
UniProt: (in Ref. 4; AAA24307);
Transmembrane-Region 310 -> 330
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b2321 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10229; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Schoenlein89: Schoenlein PV, Roa BB, Winkler ME (1989). "Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12." J Bacteriol 171(11);6084-92. PMID: 2681152

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Aug 27, 2015, biocyc14.