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Escherichia coli K-12 substr. MG1655 Polypeptide: ferric coprogen outer membrane porin FhuE



Gene: fhuE Accession Numbers: EG10306 (EcoCyc), b1102, ECK1088

Regulation Summary Diagram: ?

Component of:
ferric coprogen outer membrane transport complex
ferric coprogen transport system

Summary:
FhuE is a protein which serves as a receptor for ferric-coprogen (a hydroxamate-type iron chelator) and ferric-rhodotorulic acid [Sauer87]. Mutants lacking FhuE are unable to grow with coprogen, ferrioxamine B, ferrioxamine D1 or rhodotorulic acid [Hantke83]. Uptake into the periplasm is facilitated by TonB coupling of inner membrane energy to power specific uptake across the outer membrane [Bitter94]. Coprogen iron uptake is dependent on tonB, exbB and fhuCDB [Hantke83]. FhuE appears to have a preference for Δ-absolute configuration metal complexes [Matzanke84].

Gene Citations: [Sauer90]

Locations: outer membrane

Map Position: [1,158,585 <- 1,160,774] (24.97 centisomes)
Length: 2190 bp / 729 aa

Molecular Weight of Polypeptide: 81.232 kD (from nucleotide sequence), 76.0 kD (experimental) [Sauer87 ]

Unification Links: ASAP:ABE-0003724 , CGSC:18367 , EchoBASE:EB0302 , EcoGene:EG10306 , EcoliWiki:b1102 , ModBase:P16869 , OU-Microarray:b1102 , PortEco:fhuE , PR:PRO_000022613 , Pride:P16869 , Protein Model Portal:P16869 , RefSeq:NP_415620 , RegulonDB:EG10306 , SMR:P16869 , String:511145.b1102 , UniProt:P16869

Relationship Links: InterPro:IN-FAMILY:IPR000531 , InterPro:IN-FAMILY:IPR010105 , InterPro:IN-FAMILY:IPR010916 , InterPro:IN-FAMILY:IPR010917 , InterPro:IN-FAMILY:IPR012910 , Pfam:IN-FAMILY:PF00593 , Pfam:IN-FAMILY:PF07715 , Prosite:IN-FAMILY:PS00430 , Prosite:IN-FAMILY:PS01156

In Paralogous Gene Group: 51 (8 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11a]
GO:0044718 - siderophore transmembrane transport Inferred by computational analysis [GOA01]
GO:0055072 - iron ion homeostasis Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0004872 - receptor activity Inferred by computational analysis [GOA01]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0005506 - iron ion binding Inferred by computational analysis [GOA01]
GO:0015343 - siderophore transmembrane transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0009279 - cell outer membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Molloy00, Hantke83]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes adaptations Fe aquisition
metabolism central intermediary metabolism incorporation of metal ions
transport Channel-type Transporters Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)

Essentiality data for fhuE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 30-Oct-2011 by Mackie A , Macquarie University


Subunit of: ferric coprogen outer membrane transport complex

Subunit composition of ferric coprogen outer membrane transport complex = [FhuE][(TonB)(ExbB)(ExbD)]
         ferric coprogen outer membrane porin FhuE = FhuE (summary available)
         TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                 TonB energy transducing system - TonB subunit = TonB (extended summary available)
                 TonB energy transducing system - ExbB subunit = ExbB (summary available)
                 tonB energy transducing system - ExbD subunit = ExbD (summary available)

Component of: ferric coprogen transport system

Locations: periplasmic space, outer membrane, inner membrane

GO Terms:

Cellular Component: GO:0005886 - plasma membrane
GO:0009279 - cell outer membrane
GO:0030288 - outer membrane-bounded periplasmic space

Credits:
Created 30-Oct-2011 by Mackie A , Macquarie University


Enzymatic reaction of: transport of ferric coprogen (ferric coprogen outer membrane transport complex)


Subunit of: ferric coprogen transport system

Subunit composition of ferric coprogen transport system = [(FhuC)2(FhuB)2(FhuD)][(FhuE)([TonB][ExbB][ExbD])]
         iron (III) hydroxamate ABC transporter = (FhuC)2(FhuB)2(FhuD) (extended summary available)
                 iron (III) hydroxamate ABC transporter - ATP binding subunit = FhuC
                 iron (III) hydroxamate ABC transporter - membrane subunit = FhuB
                 iron (III) hydroxamate ABC transporter - periplasmic binding protein = FhuD (summary available)
         ferric coprogen outer membrane transport complex = (FhuE)([TonB][ExbB][ExbD])
                 ferric coprogen outer membrane porin FhuE = FhuE (summary available)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)

Credits:
Created 30-Oct-2011 by Mackie A , Macquarie University


Enzymatic reaction of: transport of ferric coprogen (ferric coprogen transport system)


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 36
[Sauer87, UniProt11]
.
Chain 37 -> 729
[UniProt09]
UniProt: FhuE receptor;
Protein-Segment 42 -> 49
[UniProt10a]
UniProt: TonB box; Sequence Annotation Type: short sequence motif;
Mutagenesis-Variant 44
[UniProt10a]
Alternate sequence: V → P; UniProt: Abolishes transport activity;
Mutagenesis-Variant 46
[UniProt10a]
Alternate sequence: V → P; UniProt: Abolishes transport activity;
Mutagenesis-Variant 163
[UniProt10a]
Alternate sequence: G → H; UniProt: Impaired transport and growth;
Alternate sequence: G → D; UniProt: Impaired transport and growth;
Alternate sequence: G → N; UniProt: Impaired transport and growth;
Alternate sequence: G → V; UniProt: Impaired transport and growth;
Alternate sequence: G → A; UniProt: Impaired transport and growth;
Sequence-Conflict 363
[Sauer90, UniProt10a]
Alternate sequence: G → C; UniProt: (in Ref. 1; CAA35616);
Protein-Segment 712 -> 729
[UniProt10a]
UniProt: TonB C-terminal box; Sequence Annotation Type: short sequence motif;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b1102 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10306; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bitter94: Bitter W, van Leeuwen IS, de Boer J, Zomer HW, Koster MC, Weisbeek PJ, Tommassen J (1994). "Localization of functional domains in the Escherichia coli coprogen receptor FhuE and the Pseudomonas putida ferric-pseudobactin 358 receptor PupA." Mol Gen Genet 1994;245(6);694-703. PMID: 7830717

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hantke83: Hantke K (1983). "Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12." Mol Gen Genet 191(2);301-6. PMID: 6353165

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Matzanke84: Matzanke BF, Muller GI, Raymond KN (1984). "Hydroxamate siderophore mediated iron uptake in E. coli: stereospecific recognition of ferric rhodotorulic acid." Biochem Biophys Res Commun 1984;121(3);922-30. PMID: 6234892

Molloy00: Molloy MP, Herbert BR, Slade MB, Rabilloud T, Nouwens AS, Williams KL, Gooley AA (2000). "Proteomic analysis of the Escherichia coli outer membrane." Eur J Biochem 267(10);2871-81. PMID: 10806384

Sauer87: Sauer M, Hantke K, Braun V (1987). "Ferric-coprogen receptor FhuE of Escherichia coli: processing and sequence common to all TonB-dependent outer membrane receptor proteins." J Bacteriol 1987;169(5);2044-9. PMID: 3032906

Sauer90: Sauer M, Hantke K, Braun V (1990). "Sequence of the fhuE outer-membrane receptor gene of Escherichia coli K12 and properties of mutants." Mol Microbiol 4(3);427-37. PMID: 2162465

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Newman99: Newman DL, Shapiro JA (1999). "Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development." Mol Microbiol 33(1);18-32. PMID: 10411720


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC13A.