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Escherichia coli K-12 substr. MG1655 Polypeptide: rod shape-determining membrane protein; sensitivity to radiation and drugs



Gene: mrdB Accession Numbers: EG10607 (EcoCyc), b0634, ECK0627

Synonyms: mdrB, rodA

Regulation Summary Diagram: ?

Summary:
The rodA gene encodes the RodA protein responsible for maintaining the rod cell shape and mecillinam sensitivity in E. coli along with pbpA, which encodes PBP2 [Tamaki80], [Iwaya78]. The rodA and pbpA genes are members of a single transcriptional unit called the rodA operon, and rodA also has its own promoter within the pbpA gene [Matsuzawa89], [Asoh83]. Cell fractionation and SDS-PAGE show that RodA is a 31 kDa, inner membrane protein that cannot enter a polyacrylamide gel after boiling in sodium dodecyl sulfate buffer due to irreversible aggregation. RodA is not made as a preprotein [Stoker83]. RodA, with its predicted 10 transmembrane segments, may somehow take part in the translocation of murein precursors [Holtje98]. Biochemical assays have shown that RodA along with PBP2 is required for proper peptidoglycan synthesis by transglycosylation, though it is unclear as to the mechanism of its contribution [Ishino86].

Experiments have been performed characterizing mutations of RodA alone and coupled with other proteins involved in septum formation [Spratt80], [Tamaki80], [Matsuzawa73], [Begg85], [Begg86], [Vinella93a], [Begg90], [Garcia91], [Begg98], [dePedro01].

Buoyand density studies of rodA mutants have been performed [Bylund91].

Locations: inner membrane

Map Position: [664,424 <- 665,536] (14.32 centisomes)
Length: 1113 bp / 370 aa

Molecular Weight of Polypeptide: 40.476 kD (from nucleotide sequence), 31 kD (experimental) [Stoker83 ]

Unification Links: ASAP:ABE-0002173 , CGSC:18145 , DIP:DIP-48062N , EchoBASE:EB0602 , EcoGene:EG10607 , EcoliWiki:b0634 , Mint:MINT-1235519 , OU-Microarray:b0634 , PortEco:mrdB , PR:PRO_000023288 , Pride:P0ABG7 , Protein Model Portal:P0ABG7 , RefSeq:NP_415167 , RegulonDB:EG10607 , String:511145.b0634 , UniProt:P0ABG7

Relationship Links: InterPro:IN-FAMILY:IPR001182 , InterPro:IN-FAMILY:IPR011923 , InterPro:IN-FAMILY:IPR018365 , Pfam:IN-FAMILY:PF01098 , Prosite:IN-FAMILY:PS00428

In Paralogous Gene Group: 587 (2 members)

GO Terms:

Biological Process: GO:0008360 - regulation of cell shape Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Spratt80]
GO:0007049 - cell cycle Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Stoker83]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell processes cell division
cell structure membrane
transport Putative uncharacterized transport protein

Essentiality data for mrdB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 20 -> 40
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 50 -> 70
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 75 -> 95
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 136 -> 156
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 160 -> 180
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 183 -> 203
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 263 -> 283
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 312 -> 332
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 336 -> 356
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0634 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10607; confirmed by SwissProt match.


References

Asoh83: Asoh S, Matsuzawa H, Matsuhashi M, Ohta T (1983). "Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids." J Bacteriol 154(1);10-6. PMID: 6300030

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Begg85: Begg KJ, Donachie WD (1985). "Cell shape and division in Escherichia coli: experiments with shape and division mutants." J Bacteriol 163(2);615-22. PMID: 3894330

Begg86: Begg KJ, Spratt BG, Donachie WD (1986). "Interaction between membrane proteins PBP3 and rodA is required for normal cell shape and division in Escherichia coli." J Bacteriol 167(3);1004-8. PMID: 3017915

Begg90: Begg KJ, Takasuga A, Edwards DH, Dewar SJ, Spratt BG, Adachi H, Ohta T, Matsuzawa H, Donachie WD (1990). "The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide." J Bacteriol 172(12);6697-703. PMID: 2254246

Begg98: Begg KJ, Donachie WD (1998). "Division planes alternate in spherical cells of Escherichia coli." J Bacteriol 180(9);2564-7. PMID: 9573213

Bylund91: Bylund JE, Haines MA, Walsh K, Bouloc P, D'Ari R, Higgins ML (1991). "Buoyant density studies of several mecillinam-resistant and division mutants of Escherichia coli." J Bacteriol 173(17);5396-402. PMID: 1885519

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

dePedro01: de Pedro MA, Donachie WD, Holtje JV, Schwarz H (2001). "Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2." J Bacteriol 183(14);4115-26. PMID: 11418550

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Garcia91: Garcia del Portillo F, de Pedro MA (1991). "Penicillin-binding protein 2 is essential for the integrity of growing cells of Escherichia coli ponB strains." J Bacteriol 173(14);4530-2. PMID: 2066344

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Holtje98: Holtje JV (1998). "Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli." Microbiol Mol Biol Rev 62(1);181-203. PMID: 9529891

Ishino86: Ishino F, Park W, Tomioka S, Tamaki S, Takase I, Kunugita K, Matsuzawa H, Asoh S, Ohta T, Spratt BG (1986). "Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein." J Biol Chem 261(15);7024-31. PMID: 3009484

Iwaya78: Iwaya M, Jones CW, Khorana J, Strominger JL (1978). "Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli." J Bacteriol 133(1);196-202. PMID: 201607

Matsuzawa73: Matsuzawa H, Hayakawa K, Sato T, Imahori K (1973). "Characterization and genetic analysis of a mutant of Escherichia coli K-12 with rounded morphology." J Bacteriol 115(1);436-42. PMID: 4577747

Matsuzawa89: Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T (1989). "Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon." J Bacteriol 171(1);558-60. PMID: 2644207

Spratt80: Spratt BG, Boyd A, Stoker N (1980). "Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome." J Bacteriol 143(2);569-81. PMID: 6451612

Stoker83: Stoker NG, Pratt JM, Spratt BG (1983). "Identification of the rodA gene product of Escherichia coli." J Bacteriol 155(2);854-9. PMID: 6348029

Tamaki80: Tamaki S, Matsuzawa H, Matsuhashi M (1980). "Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli." J Bacteriol 141(1);52-7. PMID: 6243629

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vinella93a: Vinella D, Joseleau-Petit D, Thevenet D, Bouloc P, D'Ari R (1993). "Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression." J Bacteriol 175(20);6704-10. PMID: 8407846

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.