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Escherichia coli K-12 substr. MG1655 Enzyme: O6-alkylguanine-DNA-alkyltransferase



Gene: ogt Accession Numbers: EG10668 (EcoCyc), b1335, ECK1331

Synonyms: O-6-alkylguanine-DNA/Cysteine-protein-methyltransferase

Regulation Summary Diagram: ?

Summary:
Ogt is the second (after Ada) methyltransferase enzyme for the direct repair of alkylating lesions in Escherichia coli and is responsible the background constitutive alkyl-transfer when the Ada response is not induced. Like Ada, Ogt repairs O6-methylguanine and O4-methylthymine lesions by transferring the methyl group to its own cysteine residue. Unlike Ada, Ogt is not inducible, does not transfer methyl groups from methylphosphotriesters, and does not trigger the adaptive response to alkylating agents [Potter87] [Samson92].

Locations: cytosol

Map Position: [1,397,745 <- 1,398,260] (30.13 centisomes)
Length: 516 bp / 171 aa

Molecular Weight of Polypeptide: 19.179 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004486 , CGSC:31984 , DIP:DIP-48073N , EchoBASE:EB0662 , EcoGene:EG10668 , EcoliWiki:b1335 , Mint:MINT-1243838 , ModBase:P0AFH0 , OU-Microarray:b1335 , PortEco:ogt , Pride:P0AFH0 , Protein Model Portal:P0AFH0 , RefSeq:NP_415851 , RegulonDB:EG10668 , SMR:P0AFH0 , String:511145.b1335 , Swiss-Model:P0AFH0 , UniProt:P0AFH0

Relationship Links: InterPro:IN-FAMILY:IPR001497 , InterPro:IN-FAMILY:IPR008332 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR014048 , InterPro:IN-FAMILY:IPR023546 , Pfam:IN-FAMILY:PF01035 , Pfam:IN-FAMILY:PF02870 , Prosite:IN-FAMILY:PS00374

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006281 - DNA repair Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Potter87]
GO:0006307 - DNA dealkylation involved in DNA repair Inferred by computational analysis [GOA06]
GO:0006974 - cellular response to DNA damage stimulus Inferred by computational analysis [UniProtGOA11]
GO:0032259 - methylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0003908 - methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, GOA01, Potter87]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0008168 - methyltransferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer DNA related DNA restriction/modification
regulation type of regulation DNA structure level methylation

Essentiality data for ogt knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Enzymatic reaction of: O6-alkylguanine-DNA-alkyltransferase

Synonyms: methylated-DNA-[protein]-cysteine S-methyltransferase, DNA-6-O-methylguanine:[protein]-L-cysteine S-methyltransferase, O6-alkylguanine DNA alkyltransferase, O6AG ATase

EC Number: 2.1.1.63

a [protein]-L-cysteine + a DNA containing 6-O-methylguanine <=> a [protein]-S-methyl-L-cysteine + DNA (without 6-O-methylguanine)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Reversibility of this reaction is unspecified.

T(opt): 37 °C [BRENDA14, Skorvaga98]


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 37
[Potter87, UniProt10a]
Alternate sequence: R → A; UniProt: (in Ref. 1; CAA68548);
Sequence-Conflict 68
[Potter87, UniProt10a]
Alternate sequence: E → D; UniProt: (in Ref. 1; CAA68548);
Mutagenesis-Variant 102
[Harris92, UniProt11]
Alternate sequence: C → G; UniProt: Little effect on the activity.
Mutagenesis-Variant 139
[Harris92, UniProt11]
Alternate sequence: C → G; UniProt: Eliminates the activity.
Active-Site 139
[UniProt10]
UniProt: Nucleophile; methyl group acceptor; Non-Experimental Qualifier: probable;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1335 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10668; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Harris92: Harris LC, Potter PM, Margison GP (1992). "Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein." Biochem Biophys Res Commun 187(1);425-31. PMID: 1520330

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Potter87: Potter PM, Wilkinson MC, Fitton J, Carr FJ, Brennand J, Cooper DP, Margison GP (1987). "Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli." Nucleic Acids Res 15(22);9177-93. PMID: 2825131

Samson92: Samson L (1992). "The suicidal DNA repair methyltransferases of microbes." Mol Microbiol 6(7);825-31. PMID: 1602962

Skorvaga98: Skorvaga M, Raven ND, Margison GP (1998). "Thermostable archaeal O6-alkylguanine-DNA alkyltransferases." Proc Natl Acad Sci U S A 95(12);6711-5. PMID: 9618477

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Squire09: Squire DJ, Xu M, Cole JA, Busby SJ, Browning DF (2009). "Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters." Biochem J 420(2);249-57. PMID: 19245365

Zhao10a: Zhao K, Liu M, Burgess RR (2010). "Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis." Nucleic Acids Res 38(4);1273-83. PMID: 19969540


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.