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Escherichia coli K-12 substr. MG1655 Enzyme: osmotically inducible peroxiredoxin



Gene: osmC Accession Numbers: EG10680 (EcoCyc), b1482, ECK1476

Synonyms: osmotically inducible protein

Regulation Summary Diagram: ?

Regulation summary diagram for osmC

Subunit composition of osmotically inducible peroxiredoxin = [OsmC]2
         osmotically inducible peroxiredoxin = OsmC

Summary:
OsmC from E. coli O157:H7 EDL933, which is 99% identical to the K-12 MG1622 protein, has been shown to have peroxidase activity with a strong preference for organic hydroperoxides [Lesniak03]. The catalytic activity involves oxidation of cysteine residues within the protein, which can subsequently be reduced by lipoylated proteins [Cussiol10].

The crystal structure of OsmC shows a domain-swapped dimer [Lesniak03, Shin04a].

Expression of osmC is induced before entry into stationary phase [Gordia96, Bouvier98] and by increased osmotic pressure [Gutierrez91, Toesca01]. OsmC accumulates upon osmotic stress under both aerobic and anaerobic conditions [Weber06] and in the presence of the antifouling agent zosteric acid [Villa12]. Abundance of OsmC is decreased in a yfgM deletion strain [Gotzke14]. Mutations in H-NS lead to constitutive expression of osmC [Gutierrez91]; regulation of osmC expression by H-NS may be indirect [Bouvier98].

osmC mutants show increased sensitivity to oxidative stress and reduced viability during long-term stationary phase [Conter01].

Review: [Dubbs07]

Citations: [DavalosGarcia01, Sturny03, FrancezCharlot05, Zhang12a]

Locations: cytosol

Map Position: [1,554,649 -> 1,555,080] (33.51 centisomes, 121°)
Length: 432 bp / 143 aa

Molecular Weight of Polypeptide: 15.088 kD (from nucleotide sequence), 14 kD (experimental) [Gutierrez91 ]

Unification Links: ASAP:ABE-0004944 , CGSC:32184 , DIP:DIP-48058N , EchoBASE:EB0674 , EcoGene:EG10680 , EcoliWiki:b1482 , OU-Microarray:b1482 , PortEco:osmC , PR:PRO_000023468 , Pride:P0C0L2 , Protein Model Portal:P0C0L2 , RefSeq:NP_415999 , RegulonDB:EG10680 , SMR:P0C0L2 , String:511145.b1482 , UniProt:P0C0L2

Relationship Links: InterPro:IN-FAMILY:IPR003718 , InterPro:IN-FAMILY:IPR015946 , InterPro:IN-FAMILY:IPR019904 , PDB:Structure:1NYE , PDB:Structure:1QWI , Pfam:IN-FAMILY:PF02566

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for osmC

GO Terms:

Biological Process: GO:0006972 - hyperosmotic response Inferred from experiment [Gutierrez91]
GO:0006979 - response to oxidative stress Inferred from experiment Inferred by computational analysis [GOA01a, Conter01]
GO:0033194 - response to hydroperoxide Inferred from experiment [Conter01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0004601 - peroxidase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01a, Lesniak03]
GO:0042803 - protein homodimerization activity Inferred from experiment [Lesniak03]
GO:0051920 - peroxiredoxin activity Inferred from experiment Inferred by computational analysis [GOA01, Cussiol10]
GO:0016209 - antioxidant activity Inferred by computational analysis [UniProtGOA11]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: cell processes adaptations osmotic pressure

Essentiality data for osmC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Created 19-Nov-2014 by Keseler I , SRI International
Last-Curated ? 19-Nov-2014 by Keseler I , SRI International


Enzymatic reaction of: peroxiredoxin

EC Number: 1.11.1.15

an organic hydroperoxide + a reduced thioredoxin <=> an alcohol + an oxidized thioredoxin + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for an organic hydroperoxide: cumene hydroperoxide [Lesniak03 ]


Sequence Features

Protein sequence of osmotically inducible peroxiredoxin with features indicated

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Link97, UniProt11a]
UniProt: Removed.
Chain 2 -> 143
[UniProt09]
UniProt: Peroxiredoxin osmC;
N6-acetyllysine-Modification 16
[Zhang09, UniProt15]
UniProt: N6-acetyllysine.


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b1482 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10680; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bouvier98: Bouvier J, Gordia S, Kampmann G, Lange R, Hengge-Aronis R, Gutierrez C (1998). "Interplay between global regulators of Escherichia coli: effect of RpoS, Lrp and H-NS on transcription of the gene osmC." Mol Microbiol 1998;28(5);971-80. PMID: 9663683

Conter01: Conter A, Gangneux C, Suzanne M, Gutierrez C (2001). "Survival of Escherichia coli during long-term starvation: effects of aeration, NaCl, and the rpoS and osmC gene products." Res Microbiol 152(1);17-26. PMID: 11281321

Cussiol10: Cussiol JR, Alegria TG, Szweda LI, Netto LE (2010). "Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity, lipoyl-dependent peroxidase." J Biol Chem 285(29);21943-50. PMID: 20463026

DavalosGarcia01: Davalos-Garcia M, Conter A, Toesca I, Gutierrez C, Cam K (2001). "Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli." J Bacteriol 183(20);5870-6. PMID: 11566985

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dubbs07: Dubbs JM, Mongkolsuk S (2007). "Peroxiredoxins in bacterial antioxidant defense." Subcell Biochem 44;143-93. PMID: 18084893

FrancezCharlot05: Francez-Charlot A, Castanie-Cornet MP, Gutierrez C, Cam K (2005). "Osmotic Regulation of the Escherichia coli bdm (Biofilm-Dependent Modulation) Gene by the RcsCDB His-Asp Phosphorelay." J Bacteriol 187(11);3873-7. PMID: 15901715

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gordia96: Gordia S, Gutierrez C (1996). "Growth-phase-dependent expression of the osmotically inducible gene osmC of Escherichia coli K-12." Mol Microbiol 1996;19(4);729-36. PMID: 8820643

Gotzke14: Gotzke H, Muheim C, Altelaar AF, Heck AJ, Maddalo G, Daley DO (2014). "Identification of putative substrates for the periplasmic chaperone YfgM in Escherichia coli using quantitative proteomics." Mol Cell Proteomics. PMID: 25403562

Gutierrez91: Gutierrez C, Devedjian JC (1991). "Osmotic induction of gene osmC expression in Escherichia coli K12." J Mol Biol 1991;220(4);959-73. PMID: 1715407

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lesniak03: Lesniak J, Barton WA, Nikolov DB (2003). "Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC." Protein Sci 12(12);2838-43. PMID: 14627744

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Shin04a: Shin DH, Choi IG, Busso D, Jancarik J, Yokota H, Kim R, Kim SH (2004). "Structure of OsmC from Escherichia coli: a salt-shock-induced protein." Acta Crystallogr D Biol Crystallogr 60(Pt 5);903-11. PMID: 15103136

Sturny03: Sturny R, Cam K, Gutierrez C, Conter A (2003). "NhaR and RcsB independently regulate the osmCp1 promoter of Escherichia coli at overlapping regulatory sites." J Bacteriol 185(15);4298-304. PMID: 12867437

Toesca01: Toesca I, Perard C, Bouvier J, Gutierrez C, Conter A (2001). "The transcriptional activator NhaR is responsible for the osmotic induction of osmC(p1), a promoter of the stress-inducible gene osmC in Escherichia coli." Microbiology 2001;147(Pt 10);2795-803. PMID: 11577158

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Villa12: Villa F, Remelli W, Forlani F, Vitali A, Cappitelli F (2012). "Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt." Environ Microbiol 14(7);1753-61. PMID: 22176949

Weber06: Weber A, Kogl SA, Jung K (2006). "Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli." J Bacteriol 188(20);7165-75. PMID: 17015655

Zhang09: Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8(2);215-25. PMID: 18723842

Zhang12a: Zhang Y, Shi C, Yu J, Ren J, Sun D (2012). "RpoS regulates a novel type of plasmid DNA transfer in Escherichia coli." PLoS One 7(3);e33514. PMID: 22438941

Other References Related to Gene Regulation

Bordes00: Bordes P, Repoila F, Kolb A, Gutierrez C (2000). "Involvement of differential efficiency of transcription by esigmas and esigma70 RNA polymerase holoenzymes in growth phase regulation of the Escherichia coli osmE promoter." Mol Microbiol 35(4);845-53. PMID: 10692161


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Apr 26, 2015, BIOCYC13A.