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Escherichia coli K-12 substr. MG1655 Polypeptide: recombination and repair



Gene: recR Accession Numbers: EG10834 (EcoCyc), b0472, ECK0466

Regulation Summary Diagram: ?

Component of: RecFOR complex (extended summary available)

Summary:
RecR is a component of the RecFOR complex which functions in RecA-mediated replication recovery

Locations: cytosol

Map Position: [493,629 -> 494,234] (10.64 centisomes)
Length: 606 bp / 201 aa

Molecular Weight of Polypeptide: 21.963 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001638 , CGSC:31049 , EchoBASE:EB0827 , EcoGene:EG10834 , EcoliWiki:b0472 , ModBase:P0A7H6 , OU-Microarray:b0472 , PortEco:recR , PR:PRO_000023707 , Pride:P0A7H6 , Protein Model Portal:P0A7H6 , RefSeq:NP_415005 , RegulonDB:EG10834 , SMR:P0A7H6 , String:511145.b0472 , Swiss-Model:P0A7H6 , UniProt:P0A7H6

Relationship Links: InterPro:IN-FAMILY:IPR000093 , InterPro:IN-FAMILY:IPR003583 , InterPro:IN-FAMILY:IPR006171 , InterPro:IN-FAMILY:IPR015967 , InterPro:IN-FAMILY:IPR023627 , InterPro:IN-FAMILY:IPR023628 , Pfam:IN-FAMILY:PF01751 , Pfam:IN-FAMILY:PF02132 , Prosite:IN-FAMILY:PS01300 , Prosite:IN-FAMILY:PS50880 , Smart:IN-FAMILY:SM00278 , Smart:IN-FAMILY:SM00493

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0000731 - DNA synthesis involved in DNA repair Inferred from experiment [Courcelle97]
GO:0009411 - response to UV Inferred from experiment [Courcelle97]
GO:0006281 - DNA repair Inferred by computational analysis [UniProtGOA11, GOA06, GOA01]
GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11, GOA06, GOA01]
GO:0006974 - cellular response to DNA damage stimulus Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [GOA06, GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer DNA related DNA recombination
information transfer DNA related DNA repair

Essentiality data for recR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: RecFOR complex

Subunit composition of RecFOR complex = [RecF][RecR][RecO]
         ssDNA and dsDNA binding, ATP binding = RecF (summary available)
         recombination and repair = RecR (summary available)
         protein interacts with RecR and possibly RecF proteins = RecO

Summary:
In Escherichia coli replication is temporarily inhibited after DNA damage, such as caused by UV, but resumes following a period of time commensurate with the removal of the lesions [Chow04]. Replication recovery requires RecA, which has been shown to bind to single-strand DNA, forming filaments which may protect the DNA from nuclease degradation [Courcelle01a]. The recF recO and recR gene products have also been shown, in deletion mutation studies, to serve a similar protective function [Courcelle97, Chow04]. In vitro RecF, RecO and RecR have been shown to interact with and stabilize RecA filaments bound to DNA [Courcelle97, Umezu93]. Mutants lacking RecF and RecR also experience a delay in the induction of the SOS response, consistent with their putative role in the stabilization of RecA filaments which are required for SOS induction [Chow04, Whitby95]. In vitro, RecO physically interacts with both RecF and RecR to form RecFO, RecRO or RecFRO complexes [Umezu94].

The early steps of dsDNA break repair by the RecF pathway have been studied in vitro and a model proposed [Handa09].

Credits:
Last-Curated ? 26-Jul-2009 by Mackie A , Macquarie University


Enzymatic reaction of: RecA filament-DNA complex stabilisation (RecFOR complex)

an unstable RecA filament-DNA complex <=> a stabilized RecA filament-DNA complex

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 32
[Yeung90, UniProt10a]
Alternate sequence: L → V; UniProt: (in Ref. 1; AAA23459);
Zn-Finger-Region 57 -> 72
[UniProt10]
UniProt: C4-type; Non-Experimental Qualifier: potential;
Conserved-Region 81 -> 176
[UniProt13]
UniProt: Toprim.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0472 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10834; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chow04: Chow KH, Courcelle J (2004). "RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli." J Biol Chem 279(5);3492-6. PMID: 14625283

Courcelle01a: Courcelle J, Hanawalt PC (2001). "Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination." Proc Natl Acad Sci U S A 98(15);8196-202. PMID: 11459953

Courcelle97: Courcelle J, Carswell-Crumpton C, Hanawalt PC (1997). "recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli." Proc Natl Acad Sci U S A 94(8);3714-9. PMID: 9108043

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Handa09: Handa N, Morimatsu K, Lovett ST, Kowalczykowski SC (2009). "Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli." Genes Dev 23(10);1234-45. PMID: 19451222

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Umezu93: Umezu K, Chi NW, Kolodner RD (1993). "Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein." Proc Natl Acad Sci U S A 90(9);3875-9. PMID: 8483906

Umezu94: Umezu K, Kolodner RD (1994). "Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein." J Biol Chem 269(47);30005-13. PMID: 7962001

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Whitby95: Whitby MC, Lloyd RG (1995). "Altered SOS induction associated with mutations in recF, recO and recR." Mol Gen Genet 246(2);174-9. PMID: 7862088

Yeung90: Yeung T, Mullin DA, Chen KS, Craig EA, Bardwell JC, Walker JR (1990). "Sequence and expression of the Escherichia coli recR locus." J Bacteriol 172(10);6042-7. PMID: 1698765

Other References Related to Gene Regulation

Rezuchova03: Rezuchova B, Miticka H, Homerova D, Roberts M, Kormanec J (2003). "New members of the Escherichia coli sigmaE regulon identified by a two-plasmid system." FEMS Microbiol Lett 225(1);1-7. PMID: 12900013

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13B.