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Escherichia coli K-12 substr. MG1655 Enzyme: ribosomal-protein-S5-alanine N-acetyltransferase



Gene: rimJ Accession Numbers: EG10851 (EcoCyc), b1066, ECK1051

Synonyms: tcp

Regulation Summary Diagram: ?

Regulation summary diagram for rimJ

Summary:
RimJ is an alanine acetyltransferase that is specific for 30S ribosomal subunit protein S5 [Cumberlidge79, Yoshikawa87]. RimJ also plays a role in maturation of the 30S ribosomal subunit [RoyChaudhuri08, RoyChaudhuri10].

RimJ interacts with pre-30S, but not mature ribosomal subunits [RoyChaudhuri08]. RimJ has overall similarity to RimL [Tanaka89] and C-terminal similarity to RimI [Yoshikawa87].

Overexpression of RimJ, even when lacking its acetyltransferase activity, suppresses the 16S rRNA maturation defect of an RpsE G28D mutant [RoyChaudhuri08, RoyChaudhuri10]. Overexpression of RimJ decreases the levels of the immature long precursor 16S rRNA (lp16S rRNA) in the RpsE G28D mutant [RoyChaudhuri10]. In a rimJ mutant, the ribosomal protein S5 is not acetylated at the N terminus [Cumberlidge79]. Mutation of the conserved cysteine residues C54 and/or C105 results in loss of RimJ acetyltransferase activity [RoyChaudhuri08].

RimJ acts in transcriptional regulation of the pap pilin operon by environmental conditions in uropathogenic E. coli [WhiteZiegler92, WhiteZiegler02].

Tcp: "thermoregulatory control of pap" [WhiteZiegler90].

Review: [Shajani11]

Citations: [Fang09, BernalPerez12]

Locations: cytosol

Map Position: [1,124,785 -> 1,125,369] (24.24 centisomes, 87°)
Length: 585 bp / 194 aa

Molecular Weight of Polypeptide: 22.688 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003622 , CGSC:276 , DIP:DIP-35985N , EchoBASE:EB0844 , EcoGene:EG10851 , EcoliWiki:b1066 , ModBase:P0A948 , OU-Microarray:b1066 , PortEco:rimJ , PR:PRO_000023759 , Pride:P0A948 , Protein Model Portal:P0A948 , RefSeq:NP_415584 , RegulonDB:EG10851 , SMR:P0A948 , String:511145.b1066 , UniProt:P0A948

Relationship Links: InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR016181 , Pfam:IN-FAMILY:PF00583 , Prosite:IN-FAMILY:PS51186

In Paralogous Gene Group: 248 (2 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006464 - cellular protein modification process Inferred from experiment [Cumberlidge79]
GO:0006474 - N-terminal protein amino acid acetylation Inferred from experiment [Cumberlidge79]
Molecular Function: GO:0008080 - N-acetyltransferase activity Inferred from experiment Inferred by computational analysis [GOA01a, Cumberlidge79]
GO:0008999 - ribosomal-protein-alanine N-acetyltransferase activity Inferred from experiment Inferred by computational analysis [GOA01, Cumberlidge79]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Cumberlidge79]
GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]

MultiFun Terms: information transfer protein related posttranslational modification

Essentiality data for rimJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 05-Jan-2015 by Keseler I , SRI International


Enzymatic reaction of: ribosomal-protein-S5-alanine N-acetyltransferase

Synonyms: ribosomal-protein-alanine N-acetyltransferase, ribosomal protein S5 acetyltransferase, acetyl-CoA:ribosomal-protein-L-alanine N-acetyltransferase

EC Number: 2.3.1.128

a ribosomal protein-L-alanine + acetyl-CoA <=> a ribosomal protein-N-acetyl-L-alanine + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.


Sequence Features

Protein sequence of ribosomal-protein-S5-alanine N-acetyltransferase with features indicated

Feature Class Location Citations Comment
Conserved-Region 18 -> 188
[UniProt09]
UniProt: N-acetyltransferase;
Acetylation-Modification 43
[Yu08]
 


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b1066 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10851; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BernalPerez12: Bernal-Perez LF, Sahyouni F, Prokai L, Ryu Y (2012). "RimJ-mediated context-dependent N-terminal acetylation of the recombinant Z-domain protein in Escherichia coli." Mol Biosyst 8(4);1128-30. PMID: 22293616

Cumberlidge79: Cumberlidge AG, Isono K (1979). "Ribosomal protein modification in Escherichia coli. I. A mutant lacking the N-terminal acetylation of protein S5 exhibits thermosensitivity." J Mol Biol 131(2);169-89. PMID: 385889

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fang09: Fang H, Zhang X, Shen L, Si X, Ren Y, Dai H, Li S, Zhou C, Chen H (2009). "RimJ is responsible for N(alpha)-acetylation of thymosin alpha1 in Escherichia coli." Appl Microbiol Biotechnol 84(1);99-104. PMID: 19352641

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

RoyChaudhuri08: Roy-Chaudhuri B, Kirthi N, Kelley T, Culver GM (2008). "Suppression of a cold-sensitive mutation in ribosomal protein S5 reveals a role for RimJ in ribosome biogenesis." Mol Microbiol 68(6);1547-59. PMID: 18466225

RoyChaudhuri10: Roy-Chaudhuri B, Kirthi N, Culver GM (2010). "Appropriate maturation and folding of 16S rRNA during 30S subunit biogenesis are critical for translational fidelity." Proc Natl Acad Sci U S A 107(10);4567-72. PMID: 20176963

Shajani11: Shajani Z, Sykes MT, Williamson JR (2011). "Assembly of bacterial ribosomes." Annu Rev Biochem 80;501-26. PMID: 21529161

Tanaka89: Tanaka S, Matsushita Y, Yoshikawa A, Isono K (1989). "Cloning and molecular characterization of the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli K12." Mol Gen Genet 217(2-3);289-93. PMID: 2671655

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

WhiteZiegler02: White-Ziegler CA, Black AM, Eliades SH, Young S, Porter K (2002). "The N-acetyltransferase RimJ responds to environmental stimuli to repress pap fimbrial transcription in Escherichia coli." J Bacteriol 184(16);4334-42. PMID: 12142402

WhiteZiegler90: White-Ziegler CA, Blyn LB, Braaten BA, Low DA (1990). "Identification of an Escherichia coli genetic locus involved in thermoregulation of the pap operon." J Bacteriol 172(4);1775-82. PMID: 2156803

WhiteZiegler92: White-Ziegler CA, Low DA (1992). "Thermoregulation of the pap operon: evidence for the involvement of RimJ, the N-terminal acetylase of ribosomal protein S5." J Bacteriol 174(21);7003-12. PMID: 1356970

Yoshikawa87: Yoshikawa A, Isono S, Sheback A, Isono K (1987). "Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12." Mol Gen Genet 209(3);481-8. PMID: 2828880

Yu08: Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG (2008). "The diversity of lysine-acetylated proteins in Escherichia coli." J Microbiol Biotechnol 18(9);1529-36. PMID: 18852508


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sat Aug 1, 2015, BIOCYC14B.