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Escherichia coli K-12 substr. MG1655 Polypeptide: 30S ribosomal subunit protein S1



Gene: rpsA Accession Numbers: EG10900 (EcoCyc), b0911, ECK0902

Synonyms: ssyF

Regulation Summary Diagram: ?

Component of:
30S ribosomal subunit (summary available)
ribosome (summary available)

Summary:
The S1 protein is essential in E. coli; it is a component of the ribosome and is likely required for translation of most mRNAs [Sorensen98]. During translation initiation, mRNAs are selected and bound to the ribosome with the help of two components: the conserved 3' end of 16S rRNA is complementary to the Shine-Dalgarno region of the typical mRNA, while the S1 protein binds to the leader sequence of mRNAs, upstream of the Shine-Dalgarno region [Boni91, Komarova02]. S1 promotes RNA strand displacement in vitro [Rajkowitsch07]. The S1 protein is not required for the translation of leaderless mRNAs [Tedin97, Moll02, Byrgazov12]. Association of S1 with the ribosome is unstable [Subramanian77], and the S2 protein is required for binding of S1 to the 30S subunit of the ribosome [Moll02]. The N-terminal domain of S1 is necessary and sufficient for interaction with S2 and for ribosome binding [Byrgazov12].

The S1 protein is the largest of the ribosomal proteins and assumes a complex elongated shape; it is located at the junction of the head, platform, and main body of the 30S subunit [Sengupta01]. RNA binding is associated with structural modification of the S1 protein [Aliprandi08]. NMR structures of domains 4 and 6 have been determined [Salah09].

The translation initiation region of rpsA mRNA, which encodes the S1 protein, lacks a canonical Shine-Dalgarno region, but nevertheless supports high levels of translation [Skorski06]. Expression of S1 is autoregulated; the S1 protein acts as a specific repressor of translation of its own mRNA [Christiansen81, Skouv90]. The N-terminus of S1 is sufficient for repressing rpsA expression [Skouv90]. Supported by various types of evidence including site-directed mutagenesis, footprinting and phylogenetic studies, a model was proposed in which the rpsA leader region folds into three stem-loop structures which enable efficient translation; binding of free S1 protein disturbs the conformation of the rpsA mRNA and thus specifically represses translation [Boni01, Tchufistova03].

S1 proteins carrying a C-terminal truncation due to IS10R insertion in the ssyF29 mutant lack the R4 RNA binding domain and display a growth defect [Boni00]. The growth defect was found to be due to destabilization of the rpsA mRNA [Skorski07].

The S1 protein has been suggested to play a role in trans-translation by binding transfer-messenger RNA (tmRNA) and delivering it to stalled ribosomes [Bordeau02, Karzai01, Wower00, Wower04, Okada04a]. An alternative model suggests that S1 binds to both mRNA and tmRNA molecules indiscriminately and may not play a direct role in tmRNA-mediated tagging of incompletely translated polypeptides [McGinness04]. Later studies disagree on whether S1 is [Saguy07] or is not [Qi07] required for trans-translation in vitro.

The S1 protein has been reported to bind to RNA polymerase and stimulate transcription of a number of promoters [Sukhodolets03]. S1 activates transcriptional cycling in vitro [Sukhodolets06]. S1 may also inhibit RNase E-dependent mRNA decay [Delvillani11].

ssyF: "suppressor of secY24(Ts)" [Shiba86]

Citations: [Pan11]

Gene Citations: [Weglenska96, Pedersen84, Fricke95]

Locations: ribosome, membrane, cytosol

Map Position: [961,218 -> 962,891] (20.72 centisomes)
Length: 1674 bp / 557 aa

Molecular Weight of Polypeptide: 61.158 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003104 , CGSC:230 , DIP:DIP-35884N , EchoBASE:EB0893 , EcoGene:EG10900 , EcoliWiki:b0911 , Mint:MINT-1315122 , ModBase:P0AG67 , OU-Microarray:b0911 , PortEco:rpsA , PR:PRO_000023854 , Pride:P0AG67 , Protein Model Portal:P0AG67 , RefSeq:NP_415431 , RegulonDB:EG10900 , SMR:P0AG67 , String:511145.b0911 , UniProt:P0AG67

Relationship Links: InterPro:IN-FAMILY:IPR000110 , InterPro:IN-FAMILY:IPR003029 , InterPro:IN-FAMILY:IPR012340 , InterPro:IN-FAMILY:IPR022967 , PDB:Structure:2BH8 , PDB:Structure:2KHI , PDB:Structure:2KHJ , Pfam:IN-FAMILY:PF00575 , Prints:IN-FAMILY:PR00681 , Prosite:IN-FAMILY:PS50126 , Smart:IN-FAMILY:SM00316

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006412 - translation Inferred from experiment Inferred by computational analysis [GOA01, Sorensen98]
GO:2000766 - negative regulation of cytoplasmic translation Inferred from experiment [Skouv90]
Molecular Function: GO:0003723 - RNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Wower04]
GO:0003729 - mRNA binding Inferred from experiment [Koleva06]
GO:0003735 - structural constituent of ribosome Inferred from experiment Inferred by computational analysis [GOA01, Byrgazov12]
Cellular Component: GO:0005737 - cytoplasm Inferred from experiment [Lasserre06]
GO:0016020 - membrane Inferred from experiment [Lasserre06]
GO:0022627 - cytosolic small ribosomal subunit Inferred from experiment [Hindennach71a, Subramanian77]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Regulated Transcription Units (3 total): ?

Notes:

Essentiality data for rpsA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 2]

Credits:
Last-Curated ? 21-Mar-2012 by Keseler I , SRI International


Subunit of: 30S ribosomal subunit

Synonyms: ribosome, small subunit

Subunit composition of 30S ribosomal subunit = [RrsA][RpsA][RpsB][RpsC][RpsD][RpsE][RpsF][RpsG][RpsH][RpsI][RpsJ][RpsK][RpsL][RpsM][RpsN][RpsO][RpsP][RpsQ][RpsR][RpsS][RpsT][RpsU][Sra]
         16S ribosomal RNA (rrsA) = RrsA (extended summary available)
         30S ribosomal subunit protein S1 = RpsA (extended summary available)
         30S ribosomal subunit protein S2 = RpsB (summary available)
         30S ribosomal subunit protein S3 = RpsC (summary available)
         30S ribosomal subunit protein S4 = RpsD (extended summary available)
         30S ribosomal subunit protein S5 = RpsE (extended summary available)
         30S ribosomal subunit protein S6 = RpsF (extended summary available)
         30S ribosomal subunit protein S7 = RpsG (extended summary available)
         30S ribosomal subunit protein S8 = RpsH (extended summary available)
         30S ribosomal subunit protein S9 = RpsI (extended summary available)
         30S ribosomal subunit protein S10 = RpsJ (extended summary available)
         30S ribosomal subunit protein S11 = RpsK (summary available)
         30S ribosomal subunit protein S12 = RpsL (extended summary available)
         30S ribosomal subunit protein S13 = RpsM (extended summary available)
         30S ribosomal subunit protein S14 = RpsN (summary available)
         30S ribosomal subunit protein S15 = RpsO (extended summary available)
         30S ribosomal subunit protein S16 = RpsP (summary available)
         30S ribosomal subunit protein S17 = RpsQ (summary available)
         30S ribosomal subunit protein S18 = RpsR (extended summary available)
         30S ribosomal subunit protein S19 = RpsS (summary available)
         30S ribosomal subunit protein S20 = RpsT (extended summary available)
         30S ribosomal subunit protein S21 = RpsU (summary available)
         30S ribosomal subunit protein S22 = Sra (summary available)

Component of: ribosome (summary available)

Summary:
Assembly of the 30S ribosomal subunit has been studied in real time. Initial assembly is linked to the formation of structured 16S rRNA regions, while later steps involve induced fit between ribosomal proteins and the rRNA [Adilakshmi08]. Discovery single-particle profiling was used to visualize assembly of the 30S ribosomal subunit by indentifying and following changes among 14 subunit assembly intermediates over time [Mulder10]. The kinetically favored assembly pathway of the 30S preinitiation complex has been determined [Milon12].

The function of the ribosomal P site has been reviewed [Noller05].

Relationship Links: PDB:Structure:1P6G , PDB:Structure:1P87 , PDB:Structure:2AVY

Enzymes activated by 30S ribosomal subunit, sorted by the type of activation, are:

Activator (Mechanism unknown) of: GTPase [Daigle04, Himeno04]

Credits:
Created 28-Mar-2006 by Keseler I , SRI International


Subunit of: ribosome

Subunit composition of ribosome = [(RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)][(RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)]
         30S ribosomal subunit = (RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra) (summary available)
                 16S ribosomal RNA (rrsA) = RrsA (extended summary available)
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)
                 23S ribosomal RNA (rrlA) = RrlA (extended summary available)
                 5S ribosomal RNA (rrfA) = RrfA (extended summary available)
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Summary:
The ribosome is a complex machinery that translates the genetic code.

A crystal structure of the E. coli ribosome has been determined at 3.5 Å resolution [Schuwirth05]. Additional crystal structures of the ribosome with tRNA bound in two functionally distinct states reveal how a ratchet-like motion of the small and large subunits contributes to translocation, termination of translation, and ribosome recycling [Zhang09b, Dunkle11].

Approximately eight molecules of Zn2+ are bound to the ribosome; therefore, it appears that a large fraction of intracellular Zn2+ is ribosome-associated [Hensley11].

Selected reviews: [Ramakrishnan02, Yonath05, Ogle05, Kaczanowska07]

Citations: [Kuhlenkoetter11]

Relationship Links: PDB:Structure:3R8N , PDB:Structure:3R8O , PDB:Structure:3R8S , PDB:Structure:3R8T

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 21 -> 87
[UniProt09]
UniProt: S1 motif 1;
Conserved-Region 105 -> 171
[UniProt09]
UniProt: S1 motif 2;
Sequence-Conflict 125
[Schnier82, UniProt10a]
Alternate sequence: N → D; UniProt: (in Ref. 1; AA sequence);
Sequence-Conflict 181 -> 182
[Schnier82a, UniProt10a]
Alternate sequence: ER → D; UniProt: (in Ref. 2; CAA23630/CAA23644);
Conserved-Region 192 -> 260
[UniProt09]
UniProt: S1 motif 3;
Acetylation-Modification 229
[Zhang09, UniProt11]
UniProt: N6-acetyllysine.
Acetylation-Modification 247
[Yu08]
 
Conserved-Region 277 -> 347
[UniProt09]
UniProt: S1 motif 4;
Acetylation-Modification 279
[Zhang09, UniProt11]
UniProt: N6-acetyllysine.
Acetylation-Modification 363
[Zhang09, UniProt11]
UniProt: N6-acetyllysine.
Conserved-Region 364 -> 434
[UniProt09]
UniProt: S1 motif 5;
Conserved-Region 451 -> 520
[UniProt09]
UniProt: S1 motif 6;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0911 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10900; confirmed by SwissProt match.


References

Adilakshmi08: Adilakshmi T, Bellur DL, Woodson SA (2008). "Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly." Nature 455(7217);1268-72. PMID: 18784650

Aliprandi08: Aliprandi P, Sizun C, Perez J, Mareuil F, Caputo S, Leroy JL, Odaert B, Laalami S, Uzan M, Bontems F (2008). "S1 ribosomal protein functions in translation initiation and ribonuclease RegB activation are mediated by similar RNA-protein interactions: an NMR and SAXS analysis." J Biol Chem 283(19);13289-301. PMID: 18211890

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Boni00: Boni IV, Artamonova VS, Dreyfus M (2000). "The last RNA-binding repeat of the Escherichia coli ribosomal protein S1 is specifically involved in autogenous control." J Bacteriol 182(20);5872-9. PMID: 11004188

Boni01: Boni IV, Artamonova VS, Tzareva NV, Dreyfus M (2001). "Non-canonical mechanism for translational control in bacteria: synthesis of ribosomal protein S1." EMBO J 20(15);4222-32. PMID: 11483525

Boni91: Boni IV, Isaeva DM, Musychenko ML, Tzareva NV (1991). "Ribosome-messenger recognition: mRNA target sites for ribosomal protein S1." Nucleic Acids Res 19(1);155-62. PMID: 2011495

Bordeau02: Bordeau V, Felden B (2002). "Ribosomal protein S1 induces a conformational change of tmRNA; more than one protein S1 per molecule of tmRNA." Biochimie 84(8);723-9. PMID: 12457560

Byrgazov12: Byrgazov K, Manoharadas S, Kaberdina AC, Vesper O, Moll I (2012). "Direct Interaction of the N-Terminal Domain of Ribosomal Protein S1 with Protein S2 in Escherichia coli." PLoS One 7(3);e32702. PMID: 22412910

Christiansen81: Christiansen L, Pedersen S (1981). "Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1." Mol Gen Genet 181(4);548-51. PMID: 6267426

Daigle04: Daigle DM, Brown ED (2004). "Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro." J Bacteriol 186(5);1381-7. PMID: 14973029

Delvillani11: Delvillani F, Papiani G, Deho G, Briani F (2011). "S1 ribosomal protein and the interplay between translation and mRNA decay." Nucleic Acids Res 39(17);7702-15. PMID: 21685451

Dunkle11: Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding." Science 332(6032);981-4. PMID: 21596992

Fricke95: Fricke J, Neuhard J, Kelln RA, Pedersen S (1995). "The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli." J Bacteriol 1995;177(3);517-23. PMID: 7836281

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hensley11: Hensley MP, Tierney DL, Crowder MW (2011). "Zn(II) binding to Escherichia coli 70S ribosomes." Biochemistry 50(46);9937-9. PMID: 22026583

Himeno04: Himeno H, Hanawa-Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, Mikami T, Odagiri F, Osanai Y, Watanabe D, Goto S, Kalachnyuk L, Ushida C, Muto A (2004). "A novel GTPase activated by the small subunit of ribosome." Nucleic Acids Res 32(17);5303-9. PMID: 15466596

Hindennach71a: Hindennach I, Stoffler G, Wittmann HG (1971). "Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);7-11. PMID: 4942549

Kaczanowska07: Kaczanowska M, Ryden-Aulin M (2007). "Ribosome biogenesis and the translation process in Escherichia coli." Microbiol Mol Biol Rev 71(3);477-94. PMID: 17804668

Karzai01: Karzai AW, Sauer RT (2001). "Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex." Proc Natl Acad Sci U S A 98(6);3040-4. PMID: 11248028

Koleva06: Koleva RI, Austin CA, Kowaleski JM, Neems DS, Wang L, Vary CP, Schlax PJ (2006). "Interactions of ribosomal protein S1 with DsrA and rpoS mRNA." Biochem Biophys Res Commun 348(2);662-8. PMID: 16890206

Komarova02: Komarova AV, Tchufistova LS, Supina EV, Boni IV (2002). "Protein S1 counteracts the inhibitory effect of the extended Shine-Dalgarno sequence on translation." RNA 8(9);1137-47. PMID: 12358433

Kuhlenkoetter11: Kuhlenkoetter S, Wintermeyer W, Rodnina MV (2011). "Different substrate-dependent transition states in the active site of the ribosome." Nature 476(7360);351-4. PMID: 21804565

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

McGinness04: McGinness KE, Sauer RT (2004). "Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules." Proc Natl Acad Sci U S A 101(37);13454-9. PMID: 15340139

Milon12: Milon P, Maracci C, Filonava L, Gualerzi CO, Rodnina MV (2012). "Real-time assembly landscape of bacterial 30S translation initiation complex." Nat Struct Mol Biol 19(6);609-15. PMID: 22562136

Moll02: Moll I, Grill S, Grundling A, Blasi U (2002). "Effects of ribosomal proteins S1, S2 and the DeaD/CsdA DEAD-box helicase on translation of leaderless and canonical mRNAs in Escherichia coli." Mol Microbiol 44(5);1387-96. PMID: 12068815

Mulder10: Mulder AM, Yoshioka C, Beck AH, Bunner AE, Milligan RA, Potter CS, Carragher B, Williamson JR (2010). "Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit." Science 330(6004);673-7. PMID: 21030658

Noller05: Noller HF, Hoang L, Fredrick K (2005). "The 30S ribosomal P site: a function of 16S rRNA." FEBS Lett 579(4);855-8. PMID: 15680962

Ogle05: Ogle JM, Ramakrishnan V (2005). "Structural insights into translational fidelity." Annu Rev Biochem 74;129-77. PMID: 15952884

Okada04a: Okada T, Wower IK, Wower J, Zwieb CW, Kimura M (2004). "Contribution of the second OB fold of ribosomal protein S1 from Escherichia coli to the recognition of TmRNA." Biosci Biotechnol Biochem 68(11);2319-25. PMID: 15564671

Pan11: Pan JY, Wu H, Liu X, Li PP, Li H, Wang SY, Peng XX (2011). "Complexome of Escherichia coli cytosolic proteins under normal native conditions." Mol Biosyst 7(9);2651-63. PMID: 21717022

Pedersen84: Pedersen S, Skouv J, Kajitani M, Ishihama A (1984). "Transcriptional organization of the rpsA operon of Escherichia coli." Mol Gen Genet 196(1);135-40. PMID: 6384724

Qi07: Qi H, Shimizu Y, Ueda T (2007). "Ribosomal protein S1 is not essential for the trans-translation machinery." J Mol Biol 368(3);845-52. PMID: 17376482

Rajkowitsch07: Rajkowitsch L, Schroeder R (2007). "Dissecting RNA chaperone activity." RNA 13(12);2053-60. PMID: 17901153

Ramakrishnan02: Ramakrishnan V (2002). "Ribosome structure and the mechanism of translation." Cell 108(4);557-72. PMID: 11909526

Saguy07: Saguy M, Gillet R, Skorski P, Hermann-Le Denmat S, Felden B (2007). "Ribosomal protein S1 influences trans-translation in vitro and in vivo." Nucleic Acids Res 35(7);2368-76. PMID: 17392345

Salah09: Salah P, Bisaglia M, Aliprandi P, Uzan M, Sizun C, Bontems F (2009). "Probing the relationship between Gram-negative and Gram-positive S1 proteins by sequence analysis." Nucleic Acids Res 37(16);5578-88. PMID: 19605565

Schnier82: Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittmann-Liebold B (1982). "Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA." Proc Natl Acad Sci U S A 79(4);1008-11. PMID: 7041110

Schnier82a: Schnier J, Isono K (1982). "The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1." Nucleic Acids Res 10(6);1857-65. PMID: 6281725

Schuwirth05: Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH (2005). "Structures of the bacterial ribosome at 3.5 A resolution." Science 310(5749);827-34. PMID: 16272117

Sengupta01: Sengupta J, Agrawal RK, Frank J (2001). "Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA." Proc Natl Acad Sci U S A 98(21);11991-6. PMID: 11593008

Shiba86: Shiba K, Ito K, Yura T (1986). "Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli." J Bacteriol 166(3);849-56. PMID: 3011749

Skorski06: Skorski P, Leroy P, Fayet O, Dreyfus M, Hermann-Le Denmat S (2006). "The highly efficient translation initiation region from the Escherichia coli rpsA gene lacks a shine-dalgarno element." J Bacteriol 188(17);6277-85. PMID: 16923895

Skorski07: Skorski P, Proux F, Cheraiti C, Dreyfus M, Hermann-Le Denmat S (2007). "The deleterious effect of an insertion sequence removing the last twenty percent of the essential Escherichia coli rpsA gene is due to mRNA destabilization, not protein truncation." J Bacteriol 189(17);6205-12. PMID: 17616604

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Other References Related to Gene Regulation

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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