Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein

Gene: ppdB Accession Numbers: EG11156 (EcoCyc), b2825, ECK2821

Synonyms: ygdC

Regulation Summary Diagram: ?

Regulation summary diagram for ppdB

A ppdAB mutant does not exhibit defects in chemotaxis or flagellar motility [Park01]. Overproduction of PpdAB does not result in defects in chemotaxis or flagellar motility [Park01].

PpdB: "prepilin peptidase dependent"

Regulation has been described [Park01]. Transcription is sigmaF (FliA) regulated [Park01]. Transcription is regulated by FlhDC [Park01].

Locations: cytosol, pilus

Map Position: [2,961,175 <- 2,961,738] (63.82 centisomes, 230°)
Length: 564 bp / 187 aa

Molecular Weight of Polypeptide: 20.519 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009263 , EchoBASE:EB1145 , EcoGene:EG11156 , EcoliWiki:b2825 , OU-Microarray:b2825 , PortEco:ppdB , Protein Model Portal:P08371 , RefSeq:NP_417302 , RegulonDB:EG11156 , SMR:P08371 , String:511145.b2825 , UniProt:P08371

Relationship Links: InterPro:IN-FAMILY:IPR012902 , InterPro:IN-FAMILY:IPR016419 , Pfam:IN-FAMILY:PF07963 , Prosite:IN-FAMILY:PS00409

Genetic Regulation Schematic: ?

Genetic regulation schematic for ppdB

GO Terms:

Biological Process: GO:0009101 - glycoprotein biosynthetic process
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0009289 - pilus

MultiFun Terms: cell structure pilus

Essentiality data for ppdB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of conserved protein with features indicated

Feature Class Location Citations Comment
Propeptide 1 -> 7
UniProt: Non-Experimental Qualifier: by similarity;
Methylation-Modification 8
UniProt: N-methylphenylalanine; Non-Experimental Qualifier: by similarity;
Chain 8 -> 187
UniProt: Prepilin peptidase-dependent protein B;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b2825 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11156; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Park01: Park K, Choi S, Ko M, Park C (2001). "Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus." FEMS Microbiol Lett 2001;202(2);243-50. PMID: 11520622

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc14.