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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted ATP-dependent protease

Gene: yifB Accession Numbers: EG11260 (EcoCyc), b3765, ECK3758

Regulation Summary Diagram: ?

Regulation summary diagram for yifB

YifB belongs to the Helix 2 insert clade of the AAA+ ATPases. It contains an N-terminal Lon protease domain, which may function as an ATP-dependent protease, and a unique Zn2+ cluster inserted downstream of strand 4 of the AAA+ domain [Iyer04].

Locations: cytosol

Map Position: [3,946,472 <- 3,948,022] (85.06 centisomes, 306°)
Length: 1551 bp / 516 aa

Molecular Weight of Polypeptide: 56.215 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012300 , EchoBASE:EB1240 , EcoGene:EG11260 , EcoliWiki:b3765 , ModBase:P22787 , OU-Microarray:b3765 , PortEco:yifB , Pride:P22787 , Protein Model Portal:P22787 , RefSeq:NP_418214 , RegulonDB:EG11260 , SMR:P22787 , String:511145.b3765 , UniProt:P22787

Relationship Links: InterPro:IN-FAMILY:IPR000523 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR004482 , InterPro:IN-FAMILY:IPR014721 , InterPro:IN-FAMILY:IPR020568 , InterPro:IN-FAMILY:IPR025158 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF01078 , Pfam:IN-FAMILY:PF13335 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 105 (10 members)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred by computational analysis [Iyer04]
GO:0015979 - photosynthesis Inferred by computational analysis [GOA01a]
GO:0015995 - chlorophyll biosynthetic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0004176 - ATP-dependent peptidase activity Inferred by computational analysis [Iyer04]
GO:0016851 - magnesium chelatase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: regulation type of regulation posttranscriptional proteases, cleavage of compounds

Essentiality data for yifB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 30-May-2007 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3765 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11260; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Iyer04: Iyer LM, Leipe DD, Koonin EV, Aravind L (2004). "Evolutionary history and higher order classification of AAA+ ATPases." J Struct Biol 146(1-2);11-31. PMID: 15037234

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Sep 4, 2015, biocyc13.