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Escherichia coli K-12 substr. MG1655 Polypeptide: universal stress global stress response regulator



Gene: uspA Accession Numbers: EG11390 (EcoCyc), b3495, ECK3480

Regulation Summary Diagram: ?

Summary:
UspA belongs to a superfamily of widely conserved proteins whose physiological function is unknown. Synthesis of UspA is induced in response to a variety of stresses [Nystrom92]. UspA protein is autophosphorylated at serine/threonine residues in response to the same conditions that induce its expression [Freestone97]. The overlapping and distinct roles of the various Usp proteins in E. coli have been studied [Nachin05].

A uspA null mutant has a metabolic defect, leading to a diauxic growth phenotype when diluted into fresh medium [Nystrom93]. Mutants also show a defect in survival of prolonged periods of growth inhibition [Nystrom94a] and are sensitive to UV irradiation and mitomycin C exposure [Diez00]. Overexpression of UspA leads to decreased growth rate in minimal medium and causes global changes in protein synthesis [Nystrom96].

uspA mRNA stability during glucose starvation is diminished in the absence of RNase III function [Freire06].

Transcription of uspA is repressed by FadR [Farewell96], but repression can be overcome by the alarmone ppGpp during severe cellular stress [Kvint00].

UspA: "universal stress protein A" [Nystrom92]

Reviews: [Siegele05, Kvint03]

Locations: cytosol, membrane

Map Position: [3,638,134 -> 3,638,568] (78.41 centisomes)
Length: 435 bp / 144 aa

Molecular Weight of Polypeptide: 16.066 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011414 , CGSC:28127 , DIP:DIP-36230N , EchoBASE:EB1363 , EcoGene:EG11390 , EcoliWiki:b3495 , Mint:MINT-1291607 , ModBase:P0AED0 , OU-Microarray:b3495 , PortEco:uspA , PR:PRO_000024190 , Pride:P0AED0 , Protein Model Portal:P0AED0 , RefSeq:NP_417952 , RegulonDB:EG11390 , SMR:P0AED0 , String:511145.b3495 , Swiss-Model:P0AED0 , UniProt:P0AED0

Relationship Links: InterPro:IN-FAMILY:IPR006015 , InterPro:IN-FAMILY:IPR006016 , InterPro:IN-FAMILY:IPR014729 , Pfam:IN-FAMILY:PF00582

In Paralogous Gene Group: 532 (3 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006950 - response to stress Inferred from experiment Inferred by computational analysis [GOA01a, Nystrom92]
Molecular Function: GO:0042802 - identical protein binding Inferred from experiment [Rajagopala14, Lasserre06]
Cellular Component: GO:0016020 - membrane Inferred from experiment [Lasserre06]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol

MultiFun Terms: cell processes adaptations
regulation type of regulation unknown

Essentiality data for uspA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Pasquali94, Nystrom92, Henzel93, Link97, UniProt12a]
UniProt: Removed.
Chain 2 -> 144
[UniProt09]
UniProt: Universal stress protein A;
Sequence-Conflict 140
[Nystrom92, UniProt10]
Alternate sequence: R → A; UniProt: (in Ref. 1; CAA47884);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3495 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11390; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Diez00: Diez A, Gustavsson N, Nystrom T (2000). "The universal stress protein A of Escherichia coli is required for resistance to DNA damaging agents and is regulated by a RecA/FtsK-dependent regulatory pathway." Mol Microbiol 36(6);1494-503. PMID: 10931298

Farewell96: Farewell A, Diez AA, DiRusso CC, Nystrom T (1996). "Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes." J Bacteriol 1996;178(22);6443-50. PMID: 8932299

Freestone97: Freestone P, Nystrom T, Trinei M, Norris V (1997). "The universal stress protein, UspA, of Escherichia coli is phosphorylated in response to stasis." J Mol Biol 274(3);318-24. PMID: 9405142

Freire06: Freire P, Amaral JD, Santos JM, Arraiano CM (2006). "Adaptation to carbon starvation: RNase III ensures normal expression levels of bolA1p mRNA and sigma(S)." Biochimie 88(3-4);341-6. PMID: 16309817

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Henzel93: Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993). "Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases." Proc Natl Acad Sci U S A 90(11);5011-5. PMID: 8506346

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kvint00: Kvint K, Hosbond C, Farewell A, Nybroe O, Nystrom T (2000). "Emergency derepression: stringency allows RNA polymerase to override negative control by an active repressor." Mol Microbiol 35(2);435-43. PMID: 10652104

Kvint03: Kvint K, Nachin L, Diez A, Nystrom T (2003). "The bacterial universal stress protein: function and regulation." Curr Opin Microbiol 6(2);140-5. PMID: 12732303

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Nachin05: Nachin L, Nannmark U, Nystrom T (2005). "Differential roles of the universal stress proteins of Escherichia coli in oxidative stress resistance, adhesion, and motility." J Bacteriol 187(18);6265-72. PMID: 16159758

Nystrom92: Nystrom T, Neidhardt FC (1992). "Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli." Mol Microbiol 1992;6(21);3187-98. PMID: 1453957

Nystrom93: Nystrom T, Neidhardt FC (1993). "Isolation and properties of a mutant of Escherichia coli with an insertional inactivation of the uspA gene, which encodes a universal stress protein." J Bacteriol 175(13);3949-56. PMID: 8391533

Nystrom94a: Nystrom T, Neidhardt FC (1994). "Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest." Mol Microbiol 1994;11(3);537-44. PMID: 8152377

Nystrom96: Nystrom T, Neidhardt FC (1996). "Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12." J Bacteriol 178(3);927-30. PMID: 8550536

Pasquali94: Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. (1994). Data submission to UniProtKB on 1994-09.

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Siegele05: Siegele DA (2005). "Universal stress proteins in Escherichia coli." J Bacteriol 187(18);6253-4. PMID: 16159755

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt12a: UniProt Consortium (2012). "UniProt version 2012-02 released on 2012-02-29 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Farewell98: Farewell A, Kvint K, Nystrom T (1998). "uspB, a new sigmaS-regulated gene in Escherichia coli which is required for stationary-phase resistance to ethanol." J Bacteriol 1998;180(23);6140-7. PMID: 9829921

Nystrom95: Nystrom T (1995). "Glucose starvation stimulon of Escherichia coli: role of integration host factor in starvation survival and growth phase-dependent protein synthesis." J Bacteriol 177(19);5707-10. PMID: 7559363


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14A.