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Escherichia coli K-12 substr. MG1655 Polypeptide: fatty acid/phospholipid synthesis protein



Gene: plsX Accession Numbers: EG11437 (EcoCyc), b1090, ECK1076

Regulation Summary Diagram: ?

Summary:
A mutation in plsX is required for the sn-glycerol-3-phosphate auxotrophic phenotype of a plsB mutant strain [Larson84]. A plsX ygiH double deletion is lethal [Yoshimura07a]. Yoshimura et al. hypothesize that PlsX and YgiH play a role in regulating the intracellular concentration of acyl-ACP [Yoshimura07a].

Transcript levels and operon structure of plsX have been analyzed [Podkovyrov95a, Zhang98c].

Locations: cytosol

Map Position: [1,146,844 -> 1,147,914] (24.72 centisomes)
Length: 1071 bp / 356 aa

Molecular Weight of Polypeptide: 38.214 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003692 , CGSC:18046 , DIP:DIP-10517N , EchoBASE:EB1407 , EcoGene:EG11437 , EcoliWiki:b1090 , ModBase:P27247 , OU-Microarray:b1090 , PortEco:plsX , PR:PRO_000023557 , Protein Model Portal:P27247 , RefSeq:NP_415608 , RegulonDB:EG11437 , SMR:P27247 , String:511145.b1090 , UniProt:P27247

Relationship Links: InterPro:IN-FAMILY:IPR003664 , InterPro:IN-FAMILY:IPR012281 , InterPro:IN-FAMILY:IPR024084 , Pfam:IN-FAMILY:PF02504

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0008654 - phospholipid biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Larson84]
GO:0006629 - lipid metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0006633 - fatty acid biosynthetic process Inferred by computational analysis [GOA01a]
GO:0006644 - phospholipid metabolic process Inferred by computational analysis [UniProtGOA12]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016747 - transferase activity, transferring acyl groups other than amino-acyl groups Inferred by computational analysis [GOA01, GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) phospholipid

Essentiality data for plsX knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 09-Aug-2007 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b1090 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11437; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Larson84: Larson TJ, Ludtke DN, Bell RM (1984). "sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required." J Bacteriol 160(2);711-7. PMID: 6094487

Podkovyrov95a: Podkovyrov S, Larson TJ (1995). "Lipid biosynthetic genes and a ribosomal protein gene are cotranscribed." FEBS Lett 368(3);429-31. PMID: 7635191

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Yoshimura07a: Yoshimura M, Oshima T, Ogasawara N (2007). "Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli." BMC Microbiol 7(1);69. PMID: 17645809

Zhang98c: Zhang Y, Cronan JE (1998). "Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster." J Bacteriol 180(13);3295-303. PMID: 9642179

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Podkovyrov96: Podkovyrov SM, Larson TJ (1996). "Identification of promoter and stringent regulation of transcription of the fabH, fabD and fabG genes encoding fatty acid biosynthetic enzymes of Escherichia coli." Nucleic Acids Res 1996;24(9);1747-52. PMID: 8649995

Tanaka89a: Tanaka Y, Tsujimura A, Fujita N, Isono S, Isono K (1989). "Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30-kilodalton protein." J Bacteriol 1989;171(10);5707-12. PMID: 2477362


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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