Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: predicted oxidoreductase



Gene: yciK Accession Numbers: EG11759 (EcoCyc), b1271, ECK1265

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on July 9, 2007.

Locations: cytosol

Map Position: [1,326,378 <- 1,327,136] (28.59 centisomes)
Length: 759 bp / 252 aa

Molecular Weight of Polypeptide: 27.933 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004263 , EchoBASE:EB1709 , EcoGene:EG11759 , EcoliWiki:b1271 , ModBase:P31808 , OU-Microarray:b1271 , PortEco:yciK , Pride:P31808 , Protein Model Portal:P31808 , RefSeq:NP_415787 , RegulonDB:EG11759 , SMR:P31808 , String:511145.b1271 , UniProt:P31808

Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020904 , PDB:Structure:3E9Q , PDB:Structure:3F1K , PDB:Structure:3F1L , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00081 , Prosite:IN-FAMILY:PS00061

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA et al., 2001]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProt-GOA, 2011]
Molecular Function: GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [Diaz-Mejia et al., 2009]

Gene Class: ORFs

Essentiality data for yciK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce et al., 2006, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 1]

Credits:
Last-Curated ? 09-Jul-2007 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 16 -> 40
[UniProt10]
UniProt: NADP; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 63
[Baird91, UniProt, 2010]
Alternate sequence: Q → P; UniProt: (in Ref. 5);
Amino-Acid-Sites-That-Bind 152
[UniProt10]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Active-Site 165
[UniProt10]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b1271 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11759; confirmed by SwissProt match.


References

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Baird91: Baird L, Lipinska B, Raina S, Georgopoulos C (1991). "Identification of the Escherichia coli sohB gene, a multicopy suppressor of the HtrA (DegP) null phenotype." J Bacteriol 173(18);5763-70. PMID: 1885549

Diaz-Mejia et al., 2009: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA et al., 2001: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce et al., 2006: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt-GOA, 2011: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc11.