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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted oxidoreductase



Gene: yciK Accession Numbers: EG11759 (EcoCyc), b1271, ECK1265

Regulation Summary Diagram: ?

Regulation summary diagram for yciK

Summary:
No information about this protein was found by a literature search conducted on May 7, 2015.

Locations: cytosol

Map Position: [1,326,378 <- 1,327,136] (28.59 centisomes, 103°)
Length: 759 bp / 252 aa

Molecular Weight of Polypeptide: 27.933 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004263 , EchoBASE:EB1709 , EcoGene:EG11759 , EcoliWiki:b1271 , ModBase:P31808 , OU-Microarray:b1271 , PortEco:yciK , Pride:P31808 , Protein Model Portal:P31808 , RefSeq:NP_415787 , RegulonDB:EG11759 , SMR:P31808 , String:511145.b1271 , UniProt:P31808

Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020904 , PDB:Structure:3E9Q , PDB:Structure:3F1K , PDB:Structure:3F1L , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00081 , Prosite:IN-FAMILY:PS00061

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for yciK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 07-May-2015 by Keseler I , SRI International


Sequence Features

Protein sequence of predicted oxidoreductase with features indicated

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 16 -> 40
[UniProt10a]
UniProt: NADP; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 63
[Baird91, UniProt10]
UniProt: (in Ref. 5);
Amino-Acid-Sites-That-Bind 152
[UniProt10a]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Active-Site 165
[UniProt10a]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b1271 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11759; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Baird91: Baird L, Lipinska B, Raina S, Georgopoulos C (1991). "Identification of the Escherichia coli sohB gene, a multicopy suppressor of the HtrA (DegP) null phenotype." J Bacteriol 173(18);5763-70. PMID: 1885549

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Aug 31, 2015, BIOCYC14B.