Escherichia coli K-12 substr. MG1655 Polypeptide: hydrogenase 2-specific chaperone

Gene: hybE Accession Numbers: EG11803 (EcoCyc), b2992, ECK2986

Regulation Summary Diagram: ?

Regulation summary diagram for hybE

HybE plays a role in coordinating assembly and export of the hydrogenase 2 subunits HybO and HybC. HybE physically interacts with Tat signal peptide-containing HybO [Dubini03, Chan09] and HybC [Dubini03] precursor proteins, which are Tat export substrates.

A solution structure of HybE shows that the protein adopts a three-layer sandwich fold with a disordered C-terminal region [Shao09].

A hybE deletion strain has very low hydrogenase 2 activity; in this strain, the HybO subunit is associated with the membrane, but HybC, although C-terminally processed, remains in the cytoplasm [Jack04].

Reviews: [Jack05, Forzi07]

Gene Citations: [Sargent98, Menon94]

Map Position: [3,138,333 <- 3,138,821] (67.64 centisomes, 244°)
Length: 489 bp / 162 aa

Molecular Weight of Polypeptide: 17.964 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009824 , CGSC:33424 , DIP:DIP-35795N , EchoBASE:EB1751 , EcoGene:EG11803 , EcoliWiki:b2992 , Mint:MINT-1305432 , OU-Microarray:b2992 , PortEco:hybE , PR:PRO_000022951 , Protein Model Portal:P0AAN1 , RefSeq:NP_417466 , RegulonDB:EG11803 , SMR:P0AAN1 , String:511145.b2992 , UniProt:P0AAN1

Relationship Links: InterPro:IN-FAMILY:IPR023994 , PDB:Structure:2KC5 , Pfam:IN-FAMILY:PF11939 , ProDom:IN-FAMILY:PD008308

Genetic Regulation Schematic: ?

Genetic regulation schematic for hybE

GO Terms:

Biological Process: GO:0051604 - protein maturation Inferred from experiment [Jack04]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Chan10, Chan09, Butland06, Butland05]
GO:0070678 - preprotein binding Inferred from experiment [Dubini03]

MultiFun Terms: information transfer protein related chaperoning, repair (refolding)

Essentiality data for hybE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 18-May-2009 by Keseler I , SRI International

Sequence Features

Protein sequence of hydrogenase 2-specific chaperone with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 7
[Menon94, UniProt10a]
UniProt: (in Ref. 1; AAA21593);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2992 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11803; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Butland06: Butland G, Zhang JW, Yang W, Sheung A, Wong P, Greenblatt JF, Emili A, Zamble DB (2006). "Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation." FEBS Lett 580(2);677-81. PMID: 16412426

Chan09: Chan CS, Chang L, Rommens KL, Turner RJ (2009). "Differential interactions between Tat-specific redox enzyme peptides and their chaperones." J Bacteriol 191(7):2091-101. PMID: 19151138

Chan10: Chan CS, Chang L, Winstone TM, Turner RJ (2010). "Comparing system-specific chaperone interactions with their Tat dependent redox enzyme substrates." FEBS Lett 584(22);4553-8. PMID: 20974141

Dubini03: Dubini A, Sargent F (2003). "Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins." FEBS Lett 549(1-3);141-6. PMID: 12914940

Forzi07: Forzi L, Sawers RG (2007). "Maturation of [NiFe]-hydrogenases in Escherichia coli." Biometals 20(3-4):565-78. PMID: 17216401

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Jack04: Jack RL, Buchanan G, Dubini A, Hatzixanthis K, Palmer T, Sargent F (2004). "Coordinating assembly and export of complex bacterial proteins." EMBO J 23(20);3962-72. PMID: 15385959

Jack05: Jack RL, Dubini A, Palmer T, Sargent F (2005). "Common principles in the biosynthesis of diverse enzymes." Biochem Soc Trans 33(Pt 1);105-7. PMID: 15667278

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Menon94: Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, Peck HD, Przybyla AE (1994). "Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." J Bacteriol 176(14);4416-23. PMID: 8021226

Sargent98: Sargent F, Ballantine SP, Rugman PA, Palmer T, Boxer DH (1998). "Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant." Eur J Biochem 1998;255(3);746-54. PMID: 9738917

Shao09: Shao X, Lu J, Hu Y, Xia B, Jin C (2009). "Solution structure of the Escherichia coli HybE reveals a novel fold." Proteins 75(4);1051-1056. PMID: 19291739

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

Other References Related to Gene Regulation

Chung13: Chung D, Park D, Myers K, Grass J, Kiley P, Landick R, Keles S (2013). "dPeak: high resolution identification of transcription factor binding sites from PET and SET ChIP-Seq data." PLoS Comput Biol 9(10);e1003246. PMID: 24146601

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Richard99: Richard DJ, Sawers G, Sargent F, McWalter L, Boxer DH (1999). "Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli." Microbiology 145 ( Pt 10);2903-12. PMID: 10537212

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, BIOCYC11A.