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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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Escherichia coli K-12 substr. MG1655 Polypeptide: frv operon protein



Gene: frvX Accession Numbers: EG11862 (EcoCyc), b3898, ECK3891

Synonyms: yiiI

Regulation Summary Diagram: ?

Summary:
frvX is a member of the frvABXR operon which encodes a putative fructose-specific PEP-dependent sugar phosphotransferase system [Reizer94a]. frvX is predicted to encode a water soluble protein of unknown function [Reizer94a]. Experiments with plasmid-borne lux-promoter fusions show frvX is upregulated during growth with sulfometuron methyl, which inhibits the first enzyme of valine and isoleucine biosynthesis [Van98]. frvX was also shown to be upregulated during growth with nalidixic acid [Van01].

Map Position: [4,087,878 <- 4,088,948] (88.11 centisomes)
Length: 1071 bp / 356 aa

Molecular Weight of Polypeptide: 38.733 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012722 , DIP:DIP-9694N , EchoBASE:EB1808 , EcoGene:EG11862 , EcoliWiki:b3898 , ModBase:P32153 , OU-Microarray:b3898 , PortEco:frvX , Pride:P32153 , Protein Model Portal:P32153 , RefSeq:NP_418334 , RegulonDB:EG11862 , SMR:P32153 , String:511145.b3898 , Swiss-Model:P32153 , UniProt:P32153

Relationship Links: InterPro:IN-FAMILY:IPR008007 , InterPro:IN-FAMILY:IPR023367 , Pfam:IN-FAMILY:PF05343

In Paralogous Gene Group: 81 (7 members)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0004177 - aminopeptidase activity Inferred by computational analysis [UniProtGOA11]
GO:0008233 - peptidase activity Inferred by computational analysis [UniProtGOA11]
GO:0008237 - metallopeptidase activity Inferred by computational analysis [UniProtGOA11]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: metabolism degradation of macromolecules polysaccharides

Essentiality data for frvX knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 61
[UniProt10a]
UniProt: Divalent metal cation 1; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 175
[UniProt10a]
UniProt: Divalent metal cation 1; Non-Experimental Qualifier: by similarity;
Active-Site 205
[UniProt10a]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 206
[UniProt10a]
UniProt: Divalent metal cation 2; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 228
[UniProt10a]
UniProt: Divalent metal cation 1; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 316
[UniProt10a]
UniProt: Divalent metal cation 2; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3898 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11862; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Reizer94a: Reizer J, Michotey V, Reizer A, Saier MH (1994). "Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems." Protein Sci 3(3);440-50. PMID: 8019415

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Van01: Van Dyk TK, DeRose EJ, Gonye GE (2001). "LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains." J Bacteriol 183(19);5496-505. PMID: 11544210

Van98: Van Dyk TK, Ayers BL, Morgan RW, Larossa RA (1998). "Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification." J Bacteriol 180(4);785-92. PMID: 9473030


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.