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Escherichia coli K-12 substr. MG1655 Polypeptide: membrane glycosyltransferase; synthesis of osmoregulated periplasmic glucans (OPGs)



Gene: opgH Accession Numbers: EG11886 (EcoCyc), b1049, ECK1035

Synonyms: mdoA, mdoH

Regulation Summary Diagram: ?

Summary:
The OpgH protein is a glycosyltransferase involved in the synthesis of osmoregulated periplasmic glucans (OPGs) formerly known as membrane derived oligosaccharides (MDOs).

A mutant of the mdoA locus (mdoG and/or mdoH, now called opgG and opgH) exhibits a defect in the membrane subunit(s) of the glucosyl transferase system that acts in biosynthesis of membrane-derived oligosaccharides, which are localized to the periplasmic space [Weissborn84, Bohin84]. An mdoA mutant exhibits a larger periplasmic space than wild type [Holtje88]. An mdoA mutant exhibits resistance to the bacteriophage MS2 membrane-associated lysis protein [Holtje88]. An mdoA mutant exhibits partial derepression of the sigmaS-dependent osmY transcription [Bohringer95]. An mdoA mutant also exhibits increased sensitivity to SDS, compared to wild type [Rajagopal03]. Multiple OPG deficient mutants have been characterized [Fiedler88, Weissborn92, BontempsGallo13].

High-copy overexpression of the mdoA locus results in increased OPG content, compared to wild type [Lacroix89]. UDP-glucose is an intermediate in the production of OPGs - mutant strains of E. coli that cannot produce UDP-glucose cannot synthesize OPGs [Schulman77]. A transposon insertion inactivating opgH causes increased expression of the capsular polysaccharide colanic acid [Ebel97].

OpgH contains 8 transmembrane domains. The N and C-termini are located in the cytoplasm, as is a third domain between the second and third transmembrane regions [Debarbieux97].

The mdoA locus contains the opgGH operon, which exhibits osmoregulation. Transcription is reduced upon an increase in external osmolarity. The operon does not appear to be regulated by osmoregulated periplasmic glucans [Lacroix91].

opgH: osmoregulated periplasmic glucans
mdoH: membrane derived oligosaccharides

Review: [Bohin00]

Gene Citations: [Loubens93, Rhodius05]

Locations: inner membrane

Map Position: [1,110,086 -> 1,112,629] (23.93 centisomes)
Length: 2544 bp / 847 aa

Molecular Weight of Polypeptide: 96.937 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003560 , CGSC:31852 , EchoBASE:EB1832 , EcoGene:EG11886 , EcoliWiki:b1049 , ModBase:P62517 , OU-Microarray:b1049 , PortEco:mdoH , PR:PRO_000023181 , Pride:P62517 , Protein Model Portal:P62517 , RefSeq:NP_415567 , RegulonDB:EG11886 , String:511145.b1049 , UniProt:P62517

Relationship Links: InterPro:IN-FAMILY:IPR001173 , InterPro:IN-FAMILY:IPR023725 , Pfam:IN-FAMILY:PF00535

In Paralogous Gene Group: 245 (2 members)

GO Terms:

Biological Process: GO:0006970 - response to osmotic stress Inferred from experiment [Ebel97]
GO:0009250 - glucan biosynthetic process Inferred from experiment Inferred by computational analysis [GOA06, Ebel97]
GO:0051274 - beta-glucan biosynthetic process Inferred from experiment [Weissborn84, Bohin84]
Molecular Function: GO:0016757 - transferase activity, transferring glycosyl groups Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Weissborn92]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016758 - transferase activity, transferring hexosyl groups Inferred by computational analysis [GOA06, GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, DiazMejia09, Zhang07, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Debarbieux97]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes adaptations osmotic pressure
cell structure membrane

Essentiality data for opgH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 19
[Loubens93, UniProt10a]
Alternate sequence: A → G; UniProt: (in Ref. 1; CAA45522);
Transmembrane-Region 140 -> 160
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 194 -> 214
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 289
[Loubens93, UniProt10a]
Alternate sequence: P → L; UniProt: (in Ref. 1; CAA45522);
Transmembrane-Region 513 -> 533
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 570 -> 590
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 603 -> 625
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 680 -> 700
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Amanda Mackie on Thu Dec 19, 2013:
Membrane derived oligosaccharides (MDOs) are now called osmoregulated periplasmic proteins (OPGs) and the gene names have been changed accordingly 20.12.2013
10/20/97 Gene b1049 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11886; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bohin00: Bohin JP (2000). "Osmoregulated periplasmic glucans in Proteobacteria." FEMS Microbiol Lett 186(1);11-9. PMID: 10779706

Bohin84: Bohin JP, Kennedy EP (1984). "Mapping of a locus (mdoA) that affects the biosynthesis of membrane-derived oligosaccharides in Escherichia coli." J Bacteriol 157(3);956-7. PMID: 6365896

Bohringer95: Bohringer J, Fischer D, Mosler G, Hengge-Aronis R (1995). "UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli." J Bacteriol 177(2);413-22. PMID: 7814331

BontempsGallo13: Bontemps-Gallo S, Cogez V, Robbe-Masselot C, Quintard K, Dondeyne J, Madec E, Lacroix JM (2013). "Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia coli: The Phosphoethanolamine Transferase Is Encoded by opgE." Biomed Res Int 2013;371429. PMID: 24228245

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Debarbieux97: Debarbieux L, Bohin A, Bohin JP (1997). "Topological analysis of the membrane-bound glucosyltransferase, MdoH, required for osmoregulated periplasmic glucan synthesis in Escherichia coli." J Bacteriol 179(21);6692-8. PMID: 9352918

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ebel97: Ebel W, Vaughn GJ, Peters HK, Trempy JE (1997). "Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli." J Bacteriol 179(21);6858-61. PMID: 9352941

Fiedler88: Fiedler W, Rotering H (1988). "Properties of Escherichia coli mutants lacking membrane-derived oligosaccharides." J Biol Chem 263(29);14684-9. PMID: 3049584

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Holtje88: Holtje JV, Fiedler W, Rotering H, Walderich B, van Duin J (1988). "Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides." J Biol Chem 263(8);3539-41. PMID: 3279025

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lacroix89: Lacroix JM, Tempete M, Menichi B, Bohin JP (1989). "Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli." Mol Microbiol 3(9);1173-82. PMID: 2552262

Lacroix91: Lacroix JM, Loubens I, Tempete M, Menichi B, Bohin JP (1991). "The mdoA locus of Escherichia coli consists of an operon under osmotic control." Mol Microbiol 1991;5(7);1745-53. PMID: 1834913

Loubens93: Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP (1993). "Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae." Mol Microbiol 1993;10(2);329-40. PMID: 7934824

Rajagopal03: Rajagopal S, Eis N, Bhattacharya M, Nickerson KW (2003). "Membrane-derived oligosaccharides (MDOs) are essential for sodium dodecyl sulfate resistance in Escherichia coli." FEMS Microbiol Lett 223(1);25-31. PMID: 12798996

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

Schulman77: Schulman H, Kennedy EP (1977). "Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli." J Biol Chem 252(18);6299-303. PMID: 330527

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Weissborn84: Weissborn AC, Kennedy EP (1984). "Biosynthesis of membrane-derived oligosaccharides. Novel glucosyltransferase system from Escherichia coli for the elongation of beta 1----2-linked polyglucose chains." J Biol Chem 259(20);12644-51. PMID: 6238027

Weissborn92: Weissborn AC, Rumley MK, Kennedy EP (1992). "Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides." J Bacteriol 174(14);4856-9. PMID: 1320618

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Dartigalongue01: Dartigalongue C, Missiakas D, Raina S (2001). "Characterization of the Escherichia coli sigma E regulon." J Biol Chem 276(24);20866-75. PMID: 11274153

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc12.