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Escherichia coli K-12 substr. MG1655 Polypeptide: fused predicted PTS enzyme : HPr component / enzyme I component / enzyme IIA component



Gene: ptsA Accession Numbers: EG11906 (EcoCyc), b3947, ECK3939

Synonyms: yijH, frwA, pt1A, Enzyme IAni, TTP, triphosphoryl transfer protein

Regulation Summary Diagram: ?

Component of: predicted PTS permease FrwCBD (summary available)

Summary:
Sequence analysis indicates that ptsA encodes a protein with similarity to sugar phosphotransferase system (PTS) components. The 560 residue N-terminal domain is similar to Enzymes I of the PTS and is more similar to Enzyme I's from gram positive bacteria than to Enzyme I's from gram negative bacteria. This domain contains a conserved histidine residue (his301) predicted to be the site of phosphorylation. The C-terminal domain of PtsA has similarity to the IIA domains of the PTS Enzymes II specific for fructose [Reizer95]. ptsA is predicted to encode a triphosphoryl transfer protein with the domain order H-I-IIA. ptsA encodes a hybrid protein containing an HPr-like domain, an Enzyme I-like domain and an Enzyme IIAFrw domain. PtsA is homologous to FryA throughout its length [Tchieu01].

Expression of ptsA is induced in cells treated with the supernatant from a stationary phase culture of E. coli [Ren04b].

Locations: cytosol

Map Position: [4,137,743 <- 4,140,244] (89.18 centisomes)
Length: 2502 bp / 833 aa

Molecular Weight of Polypeptide: 91.774 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012920 , DIP:DIP-10601N , EchoBASE:EB1851 , EcoGene:EG11906 , EcoliWiki:b3947 , ModBase:P32670 , OU-Microarray:b3947 , PortEco:ptsA , Pride:P32670 , Protein Model Portal:P32670 , RefSeq:YP_026278 , RegulonDB:EG11906 , SMR:P32670 , String:511145.b3947 , Swiss-Model:P32670 , UniProt:P32670

Relationship Links: InterPro:IN-FAMILY:IPR000032 , InterPro:IN-FAMILY:IPR000121 , InterPro:IN-FAMILY:IPR001020 , InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR005698 , InterPro:IN-FAMILY:IPR006318 , InterPro:IN-FAMILY:IPR008279 , InterPro:IN-FAMILY:IPR008731 , InterPro:IN-FAMILY:IPR015813 , InterPro:IN-FAMILY:IPR016152 , InterPro:IN-FAMILY:IPR018274 , InterPro:IN-FAMILY:IPR023151 , Panther:IN-FAMILY:PTHR22931:SF10 , Pfam:IN-FAMILY:PF00359 , Pfam:IN-FAMILY:PF00381 , Pfam:IN-FAMILY:PF00391 , Pfam:IN-FAMILY:PF02896 , Pfam:IN-FAMILY:PF05524 , Prints:IN-FAMILY:PR01736 , Prosite:IN-FAMILY:PS00369 , Prosite:IN-FAMILY:PS00370 , Prosite:IN-FAMILY:PS00589 , Prosite:IN-FAMILY:PS00742 , Prosite:IN-FAMILY:PS51094 , Prosite:IN-FAMILY:PS51350

In Paralogous Gene Group: 259 (4 members) , 187 (14 members) , 336 (3 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01a, Reizer95]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11, GOA01a]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0008965 - phosphoenolpyruvate-protein phosphotransferase activity Inferred by computational analysis [GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Accessory Factors Involved in Transport

Essentiality data for ptsA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 23-Mar-2011 by Keseler I , SRI International


Subunit of: predicted PTS permease FrwCBD

Synonyms: EIIFrw, Enzyme IIFrw

Subunit composition of predicted PTS permease FrwCBD = [FrwC][FrwB][FrwD][PtsA]
         predicted PTS permease - FrwC subunit = FrwC (summary available)
         predicted PTS permease - FrwB subunit = FrwB (summary available)
         predicted PTS permease - FrwD subunit = FrwD (summary available)
         fused predicted PTS enzyme : HPr component / enzyme I component / enzyme IIA component = PtsA (extended summary available)

Summary:
FrwCBDPtsA is a putative PTS permease [Tchieu01] which belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed by [Postma93]).

FrwC, FrwB, FrwD and PtsA (formerly FrwA) are all encoded within the frw gene cluster. The Frw proteins and protein domains are homologous to constituents of the fructose Enzyme II complexes. The frw gene cluster also encodes several enzymes concerned with anaerobic carbon metabolism [Reizer95]. FrwCBDPtsA is a member of the PTS Fructose-Mannitol (Fru) family of transporters [Saier14].

Please note that the presence of an frwCBDptsA encoded protein complex is hypothetical.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer95, Tchieu01]

Credits:
Revised 30-Apr-2014 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 2 -> 91
[UniProt09]
UniProt: HPr;
Active-Site 16
[UniProt10]
UniProt: Tele-phosphohistidine intermediate; for HPr activity; Non-Experimental Qualifier: by similarity;
Protein-Segment 269 -> 616
[UniProt10]
UniProt: PTS EI; Sequence Annotation Type: region of interest;
Active-Site 301
[UniProt10, Reizer95]
UniProt: Tele-phosphohistidine intermediate; for PTS EI activity; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 408
[UniProt10]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 444
[UniProt10]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 543
[UniProt10]
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 564
[UniProt10]
UniProt: Substrate; via carbonyl oxygen; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 565
[UniProt10]
UniProt: Substrate; via amide nitrogen; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 566
[UniProt10]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 567
[UniProt10]
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;
Active-Site 614
[UniProt10]
UniProt: Proton donor; for EI activity; Non-Experimental Qualifier: by similarity;
Conserved-Region 688 -> 830
[UniProt09]
UniProt: PTS EIIA type-2;
Active-Site 750
[UniProt10]
UniProt: Tele-phosphohistidine intermediate; for PTS EIIA activity; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3947 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11906; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer95: Reizer J, Reizer A, Saier MH (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4);961-71. PMID: 7773398

Ren04b: Ren D, Bedzyk LA, Ye RW, Thomas SM, Wood TK (2004). "Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli." Appl Environ Microbiol 70(4);2038-43. PMID: 15066794

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Tchieu01: Tchieu JH, Norris V, Edwards JS, Saier MH (2001). "The complete phosphotransferase system in Escherichia coli." J Mol Microbiol Biotechnol 3(3);329-46. PMID: 11361063

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc14.