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discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Escherichia coli K-12 substr. MG1655 Polypeptide: probable pyruvate formate lyase 2 activating enzyme



Gene: pflC Accession Numbers: EG11911 (EcoCyc), b3952, ECK3943

Synonyms: yijM

Regulation Summary Diagram: ?

Summary:
PflC was identified by sequence similarity as a homolog of pyruvate formate-lyase activating enzyme [Reizer95]. Effects of a gene knockout have been studied; the fermentation pattern under microaerobic conditions is similar to wild type [Zhu04].

Gene Citations: [Drapal95]

Locations: cytosol

Map Position: [4,144,281 -> 4,145,159] (89.32 centisomes)
Length: 879 bp / 292 aa

Molecular Weight of Polypeptide: 32.43 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012937 , CGSC:35847 , EchoBASE:EB1855 , EcoGene:EG11911 , EcoliWiki:b3952 , ModBase:P32675 , OU-Microarray:b3952 , PortEco:pflC , PR:PRO_000023518 , Pride:P32675 , Protein Model Portal:P32675 , RefSeq:NP_418387 , RegulonDB:EG11911 , SMR:P32675 , String:511145.b3952 , UniProt:P32675

Relationship Links: InterPro:IN-FAMILY:IPR001989 , InterPro:IN-FAMILY:IPR006638 , InterPro:IN-FAMILY:IPR007197 , InterPro:IN-FAMILY:IPR012839 , InterPro:IN-FAMILY:IPR017896 , Pfam:IN-FAMILY:PF04055 , Prosite:IN-FAMILY:PS01087 , Prosite:IN-FAMILY:PS51379 , Smart:IN-FAMILY:SM00729

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0006006 - glucose metabolic process Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0043365 - [formate-C-acetyltransferase]-activating enzyme activity Inferred by computational analysis [GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0051539 - 4 iron, 4 sulfur cluster binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA01a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related posttranslational modification
metabolism energy metabolism, carbon anaerobic respiration
regulation type of regulation posttranscriptional inhibition / activation of enzymes

Essentiality data for pflC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 47
[UniProt10]
UniProt: Iron-sulfur (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 51
[UniProt10]
UniProt: Iron-sulfur (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 54
[UniProt10]
UniProt: Iron-sulfur (4Fe-4S-S-AdoMet); Non-Experimental Qualifier: by similarity;
Conserved-Region 62 -> 96
[UniProt09]
UniProt: 4Fe-4S ferredoxin-type;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3952 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11911; confirmed by SwissProt match.


References

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Drapal95: Drapal N, Sawers G (1995). "Promoter 7 of the Escherichia coli pfl operon is a major determinant in the anaerobic regulation of expression by ArcA." J Bacteriol 1995;177(18);5338-41. PMID: 7665524

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Reizer95: Reizer J, Reizer A, Saier MH (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4);961-71. PMID: 7773398

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhu04: Zhu J, Shimizu K (2004). "The effect of pfl gene knockout on the metabolism for optically pure D-lactate production by Escherichia coli." Appl Microbiol Biotechnol 64(3);367-75. PMID: 14673546


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC14A.